[2023-06-29 17:08:43,135] [INFO] DFAST_QC pipeline started.
[2023-06-29 17:08:43,137] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 17:08:43,137] [INFO] DQC Reference Directory: /var/lib/cwl/stg45fdf14e-fe71-410a-8235-32709ff4862f/dqc_reference
[2023-06-29 17:08:44,298] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 17:08:44,299] [INFO] Task started: Prodigal
[2023-06-29 17:08:44,299] [INFO] Running command: gunzip -c /var/lib/cwl/stg75d11a57-7308-4fc0-b2b0-a88bc11e7aea/GCA_021628165.1_ASM2162816v1_genomic.fna.gz | prodigal -d GCA_021628165.1_ASM2162816v1_genomic.fna/cds.fna -a GCA_021628165.1_ASM2162816v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 17:08:48,390] [INFO] Task succeeded: Prodigal
[2023-06-29 17:08:48,390] [INFO] Task started: HMMsearch
[2023-06-29 17:08:48,391] [INFO] Running command: hmmsearch --tblout GCA_021628165.1_ASM2162816v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg45fdf14e-fe71-410a-8235-32709ff4862f/dqc_reference/reference_markers.hmm GCA_021628165.1_ASM2162816v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 17:08:48,590] [INFO] Task succeeded: HMMsearch
[2023-06-29 17:08:48,591] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg75d11a57-7308-4fc0-b2b0-a88bc11e7aea/GCA_021628165.1_ASM2162816v1_genomic.fna.gz]
[2023-06-29 17:08:48,615] [INFO] Query marker FASTA was written to GCA_021628165.1_ASM2162816v1_genomic.fna/markers.fasta
[2023-06-29 17:08:48,615] [INFO] Task started: Blastn
[2023-06-29 17:08:48,615] [INFO] Running command: blastn -query GCA_021628165.1_ASM2162816v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg45fdf14e-fe71-410a-8235-32709ff4862f/dqc_reference/reference_markers.fasta -out GCA_021628165.1_ASM2162816v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 17:08:49,209] [INFO] Task succeeded: Blastn
[2023-06-29 17:08:49,214] [INFO] Selected 20 target genomes.
[2023-06-29 17:08:49,214] [INFO] Target genome list was writen to GCA_021628165.1_ASM2162816v1_genomic.fna/target_genomes.txt
[2023-06-29 17:08:49,217] [INFO] Task started: fastANI
[2023-06-29 17:08:49,217] [INFO] Running command: fastANI --query /var/lib/cwl/stg75d11a57-7308-4fc0-b2b0-a88bc11e7aea/GCA_021628165.1_ASM2162816v1_genomic.fna.gz --refList GCA_021628165.1_ASM2162816v1_genomic.fna/target_genomes.txt --output GCA_021628165.1_ASM2162816v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 17:08:56,768] [INFO] Task succeeded: fastANI
[2023-06-29 17:08:56,768] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg45fdf14e-fe71-410a-8235-32709ff4862f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 17:08:56,769] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg45fdf14e-fe71-410a-8235-32709ff4862f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 17:08:56,771] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 17:08:56,771] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 17:08:56,771] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 17:08:56,773] [INFO] DFAST Taxonomy check result was written to GCA_021628165.1_ASM2162816v1_genomic.fna/tc_result.tsv
[2023-06-29 17:08:56,774] [INFO] ===== Taxonomy check completed =====
[2023-06-29 17:08:56,774] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 17:08:56,775] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg45fdf14e-fe71-410a-8235-32709ff4862f/dqc_reference/checkm_data
[2023-06-29 17:08:56,779] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 17:08:56,803] [INFO] Task started: CheckM
[2023-06-29 17:08:56,803] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_021628165.1_ASM2162816v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_021628165.1_ASM2162816v1_genomic.fna/checkm_input GCA_021628165.1_ASM2162816v1_genomic.fna/checkm_result
[2023-06-29 17:09:16,080] [INFO] Task succeeded: CheckM
[2023-06-29 17:09:16,082] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 85.42%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 17:09:16,101] [INFO] ===== Completeness check finished =====
[2023-06-29 17:09:16,101] [INFO] ===== Start GTDB Search =====
[2023-06-29 17:09:16,102] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_021628165.1_ASM2162816v1_genomic.fna/markers.fasta)
[2023-06-29 17:09:16,102] [INFO] Task started: Blastn
[2023-06-29 17:09:16,102] [INFO] Running command: blastn -query GCA_021628165.1_ASM2162816v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg45fdf14e-fe71-410a-8235-32709ff4862f/dqc_reference/reference_markers_gtdb.fasta -out GCA_021628165.1_ASM2162816v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 17:09:16,990] [INFO] Task succeeded: Blastn
[2023-06-29 17:09:16,995] [INFO] Selected 17 target genomes.
[2023-06-29 17:09:16,995] [INFO] Target genome list was writen to GCA_021628165.1_ASM2162816v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 17:09:16,997] [INFO] Task started: fastANI
[2023-06-29 17:09:16,997] [INFO] Running command: fastANI --query /var/lib/cwl/stg75d11a57-7308-4fc0-b2b0-a88bc11e7aea/GCA_021628165.1_ASM2162816v1_genomic.fna.gz --refList GCA_021628165.1_ASM2162816v1_genomic.fna/target_genomes_gtdb.txt --output GCA_021628165.1_ASM2162816v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 17:09:22,740] [INFO] Task succeeded: fastANI
[2023-06-29 17:09:22,746] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-29 17:09:22,747] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_001940855.1	s__Phil1 sp001940855	96.534	297	303	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Christensenellales;f__CAG-138;g__Phil1	95.0	97.09	96.81	0.92	0.85	14	conclusive
GCA_004558525.1	s__Phil1 sp004558525	80.1301	170	303	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Christensenellales;f__CAG-138;g__Phil1	95.0	98.96	98.54	0.91	0.86	6	-
--------------------------------------------------------------------------------
[2023-06-29 17:09:22,749] [INFO] GTDB search result was written to GCA_021628165.1_ASM2162816v1_genomic.fna/result_gtdb.tsv
[2023-06-29 17:09:22,749] [INFO] ===== GTDB Search completed =====
[2023-06-29 17:09:22,752] [INFO] DFAST_QC result json was written to GCA_021628165.1_ASM2162816v1_genomic.fna/dqc_result.json
[2023-06-29 17:09:22,752] [INFO] DFAST_QC completed!
[2023-06-29 17:09:22,753] [INFO] Total running time: 0h0m40s