[2023-06-29 12:38:08,330] [INFO] DFAST_QC pipeline started.
[2023-06-29 12:38:08,333] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 12:38:08,333] [INFO] DQC Reference Directory: /var/lib/cwl/stg6dc8b4ba-e100-4c51-bdc6-2a7acc1cca7f/dqc_reference
[2023-06-29 12:38:09,607] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 12:38:09,608] [INFO] Task started: Prodigal
[2023-06-29 12:38:09,608] [INFO] Running command: gunzip -c /var/lib/cwl/stgfbc2cd2b-6f39-4386-bf87-d0cb33613766/GCA_021809255.1_ASM2180925v1_genomic.fna.gz | prodigal -d GCA_021809255.1_ASM2180925v1_genomic.fna/cds.fna -a GCA_021809255.1_ASM2180925v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 12:38:15,492] [INFO] Task succeeded: Prodigal
[2023-06-29 12:38:15,493] [INFO] Task started: HMMsearch
[2023-06-29 12:38:15,493] [INFO] Running command: hmmsearch --tblout GCA_021809255.1_ASM2180925v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6dc8b4ba-e100-4c51-bdc6-2a7acc1cca7f/dqc_reference/reference_markers.hmm GCA_021809255.1_ASM2180925v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 12:38:15,788] [INFO] Task succeeded: HMMsearch
[2023-06-29 12:38:15,790] [WARNING] Found 4/6 markers. [/var/lib/cwl/stgfbc2cd2b-6f39-4386-bf87-d0cb33613766/GCA_021809255.1_ASM2180925v1_genomic.fna.gz]
[2023-06-29 12:38:15,825] [INFO] Query marker FASTA was written to GCA_021809255.1_ASM2180925v1_genomic.fna/markers.fasta
[2023-06-29 12:38:15,826] [INFO] Task started: Blastn
[2023-06-29 12:38:15,826] [INFO] Running command: blastn -query GCA_021809255.1_ASM2180925v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6dc8b4ba-e100-4c51-bdc6-2a7acc1cca7f/dqc_reference/reference_markers.fasta -out GCA_021809255.1_ASM2180925v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 12:38:16,453] [INFO] Task succeeded: Blastn
[2023-06-29 12:38:16,457] [INFO] Selected 29 target genomes.
[2023-06-29 12:38:16,457] [INFO] Target genome list was writen to GCA_021809255.1_ASM2180925v1_genomic.fna/target_genomes.txt
[2023-06-29 12:38:16,465] [INFO] Task started: fastANI
[2023-06-29 12:38:16,465] [INFO] Running command: fastANI --query /var/lib/cwl/stgfbc2cd2b-6f39-4386-bf87-d0cb33613766/GCA_021809255.1_ASM2180925v1_genomic.fna.gz --refList GCA_021809255.1_ASM2180925v1_genomic.fna/target_genomes.txt --output GCA_021809255.1_ASM2180925v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 12:38:32,037] [INFO] Task succeeded: fastANI
[2023-06-29 12:38:32,039] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6dc8b4ba-e100-4c51-bdc6-2a7acc1cca7f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 12:38:32,039] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6dc8b4ba-e100-4c51-bdc6-2a7acc1cca7f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 12:38:32,042] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 12:38:32,042] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 12:38:32,043] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 12:38:32,046] [INFO] DFAST Taxonomy check result was written to GCA_021809255.1_ASM2180925v1_genomic.fna/tc_result.tsv
[2023-06-29 12:38:32,047] [INFO] ===== Taxonomy check completed =====
[2023-06-29 12:38:32,047] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 12:38:32,047] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6dc8b4ba-e100-4c51-bdc6-2a7acc1cca7f/dqc_reference/checkm_data
[2023-06-29 12:38:32,051] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 12:38:32,087] [INFO] Task started: CheckM
[2023-06-29 12:38:32,088] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_021809255.1_ASM2180925v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_021809255.1_ASM2180925v1_genomic.fna/checkm_input GCA_021809255.1_ASM2180925v1_genomic.fna/checkm_result
[2023-06-29 12:38:57,080] [INFO] Task succeeded: CheckM
[2023-06-29 12:38:57,081] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 77.26%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 12:38:57,103] [INFO] ===== Completeness check finished =====
[2023-06-29 12:38:57,103] [INFO] ===== Start GTDB Search =====
[2023-06-29 12:38:57,104] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_021809255.1_ASM2180925v1_genomic.fna/markers.fasta)
[2023-06-29 12:38:57,104] [INFO] Task started: Blastn
[2023-06-29 12:38:57,104] [INFO] Running command: blastn -query GCA_021809255.1_ASM2180925v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6dc8b4ba-e100-4c51-bdc6-2a7acc1cca7f/dqc_reference/reference_markers_gtdb.fasta -out GCA_021809255.1_ASM2180925v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 12:38:58,049] [INFO] Task succeeded: Blastn
[2023-06-29 12:38:58,054] [INFO] Selected 30 target genomes.
[2023-06-29 12:38:58,055] [INFO] Target genome list was writen to GCA_021809255.1_ASM2180925v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 12:38:58,104] [INFO] Task started: fastANI
[2023-06-29 12:38:58,104] [INFO] Running command: fastANI --query /var/lib/cwl/stgfbc2cd2b-6f39-4386-bf87-d0cb33613766/GCA_021809255.1_ASM2180925v1_genomic.fna.gz --refList GCA_021809255.1_ASM2180925v1_genomic.fna/target_genomes_gtdb.txt --output GCA_021809255.1_ASM2180925v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 12:39:12,420] [INFO] Task succeeded: fastANI
[2023-06-29 12:39:12,425] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-29 12:39:12,426] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002423125.1	s__UBA11403 sp002423125	77.9052	72	674	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Legionellales;f__Legionellaceae;g__UBA11403	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-29 12:39:12,428] [INFO] GTDB search result was written to GCA_021809255.1_ASM2180925v1_genomic.fna/result_gtdb.tsv
[2023-06-29 12:39:12,429] [INFO] ===== GTDB Search completed =====
[2023-06-29 12:39:12,431] [INFO] DFAST_QC result json was written to GCA_021809255.1_ASM2180925v1_genomic.fna/dqc_result.json
[2023-06-29 12:39:12,431] [INFO] DFAST_QC completed!
[2023-06-29 12:39:12,431] [INFO] Total running time: 0h1m4s