[2023-06-13 20:23:33,099] [INFO] DFAST_QC pipeline started.
[2023-06-13 20:23:33,102] [INFO] DFAST_QC version: 0.5.7
[2023-06-13 20:23:33,102] [INFO] DQC Reference Directory: /var/lib/cwl/stg00083883-9124-43e2-af08-c1fdcf4b8fd8/dqc_reference
[2023-06-13 20:23:34,261] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-13 20:23:34,262] [INFO] Task started: Prodigal
[2023-06-13 20:23:34,262] [INFO] Running command: gunzip -c /var/lib/cwl/stg1799a81a-bcf1-4afe-8c89-eccba206d799/GCA_022013925.1_ASM2201392v1_genomic.fna.gz | prodigal -d GCA_022013925.1_ASM2201392v1_genomic.fna/cds.fna -a GCA_022013925.1_ASM2201392v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-13 20:23:41,498] [INFO] Task succeeded: Prodigal
[2023-06-13 20:23:41,498] [INFO] Task started: HMMsearch
[2023-06-13 20:23:41,498] [INFO] Running command: hmmsearch --tblout GCA_022013925.1_ASM2201392v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg00083883-9124-43e2-af08-c1fdcf4b8fd8/dqc_reference/reference_markers.hmm GCA_022013925.1_ASM2201392v1_genomic.fna/protein.faa > /dev/null
[2023-06-13 20:23:41,718] [INFO] Task succeeded: HMMsearch
[2023-06-13 20:23:41,720] [INFO] Found 6/6 markers.
[2023-06-13 20:23:41,751] [INFO] Query marker FASTA was written to GCA_022013925.1_ASM2201392v1_genomic.fna/markers.fasta
[2023-06-13 20:23:41,751] [INFO] Task started: Blastn
[2023-06-13 20:23:41,751] [INFO] Running command: blastn -query GCA_022013925.1_ASM2201392v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg00083883-9124-43e2-af08-c1fdcf4b8fd8/dqc_reference/reference_markers.fasta -out GCA_022013925.1_ASM2201392v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 20:23:42,345] [INFO] Task succeeded: Blastn
[2023-06-13 20:23:42,349] [INFO] Selected 24 target genomes.
[2023-06-13 20:23:42,349] [INFO] Target genome list was writen to GCA_022013925.1_ASM2201392v1_genomic.fna/target_genomes.txt
[2023-06-13 20:23:42,350] [INFO] Task started: fastANI
[2023-06-13 20:23:42,350] [INFO] Running command: fastANI --query /var/lib/cwl/stg1799a81a-bcf1-4afe-8c89-eccba206d799/GCA_022013925.1_ASM2201392v1_genomic.fna.gz --refList GCA_022013925.1_ASM2201392v1_genomic.fna/target_genomes.txt --output GCA_022013925.1_ASM2201392v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-13 20:23:56,312] [INFO] Task succeeded: fastANI
[2023-06-13 20:23:56,312] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg00083883-9124-43e2-af08-c1fdcf4b8fd8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-13 20:23:56,312] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg00083883-9124-43e2-af08-c1fdcf4b8fd8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-13 20:23:56,314] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-13 20:23:56,314] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-13 20:23:56,314] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-13 20:23:56,316] [INFO] DFAST Taxonomy check result was written to GCA_022013925.1_ASM2201392v1_genomic.fna/tc_result.tsv
[2023-06-13 20:23:56,317] [INFO] ===== Taxonomy check completed =====
[2023-06-13 20:23:56,317] [INFO] ===== Start completeness check using CheckM =====
[2023-06-13 20:23:56,317] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg00083883-9124-43e2-af08-c1fdcf4b8fd8/dqc_reference/checkm_data
[2023-06-13 20:23:56,320] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-13 20:23:56,355] [INFO] Task started: CheckM
[2023-06-13 20:23:56,355] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_022013925.1_ASM2201392v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_022013925.1_ASM2201392v1_genomic.fna/checkm_input GCA_022013925.1_ASM2201392v1_genomic.fna/checkm_result
[2023-06-13 20:24:23,612] [INFO] Task succeeded: CheckM
[2023-06-13 20:24:23,613] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.98%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-06-13 20:24:23,633] [INFO] ===== Completeness check finished =====
[2023-06-13 20:24:23,633] [INFO] ===== Start GTDB Search =====
[2023-06-13 20:24:23,633] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_022013925.1_ASM2201392v1_genomic.fna/markers.fasta)
[2023-06-13 20:24:23,634] [INFO] Task started: Blastn
[2023-06-13 20:24:23,634] [INFO] Running command: blastn -query GCA_022013925.1_ASM2201392v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg00083883-9124-43e2-af08-c1fdcf4b8fd8/dqc_reference/reference_markers_gtdb.fasta -out GCA_022013925.1_ASM2201392v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 20:24:24,447] [INFO] Task succeeded: Blastn
[2023-06-13 20:24:24,451] [INFO] Selected 22 target genomes.
[2023-06-13 20:24:24,451] [INFO] Target genome list was writen to GCA_022013925.1_ASM2201392v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-13 20:24:24,514] [INFO] Task started: fastANI
[2023-06-13 20:24:24,515] [INFO] Running command: fastANI --query /var/lib/cwl/stg1799a81a-bcf1-4afe-8c89-eccba206d799/GCA_022013925.1_ASM2201392v1_genomic.fna.gz --refList GCA_022013925.1_ASM2201392v1_genomic.fna/target_genomes_gtdb.txt --output GCA_022013925.1_ASM2201392v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-13 20:24:35,883] [INFO] Task succeeded: fastANI
[2023-06-13 20:24:35,886] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-13 20:24:35,887] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018894615.1	s__JAHIPL01 sp018894615	98.9472	657	864	d__Bacteria;p__Acidobacteriota;c__Aminicenantia;o__Aminicenantales;f__Aminicenantaceae;g__JAHIPL01	95.0	98.71	98.40	0.82	0.79	6	conclusive
--------------------------------------------------------------------------------
[2023-06-13 20:24:35,894] [INFO] GTDB search result was written to GCA_022013925.1_ASM2201392v1_genomic.fna/result_gtdb.tsv
[2023-06-13 20:24:35,894] [INFO] ===== GTDB Search completed =====
[2023-06-13 20:24:35,897] [INFO] DFAST_QC result json was written to GCA_022013925.1_ASM2201392v1_genomic.fna/dqc_result.json
[2023-06-13 20:24:35,897] [INFO] DFAST_QC completed!
[2023-06-13 20:24:35,897] [INFO] Total running time: 0h1m3s