[2023-06-13 05:12:38,381] [INFO] DFAST_QC pipeline started.
[2023-06-13 05:12:38,390] [INFO] DFAST_QC version: 0.5.7
[2023-06-13 05:12:38,393] [INFO] DQC Reference Directory: /var/lib/cwl/stg6ea1ee76-cfe3-4f99-9b08-228bd7ffd9e3/dqc_reference
[2023-06-13 05:12:40,043] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-13 05:12:40,044] [INFO] Task started: Prodigal
[2023-06-13 05:12:40,044] [INFO] Running command: gunzip -c /var/lib/cwl/stgf839cd96-8488-4120-9114-04f0cddcc320/GCA_022072225.1_ASM2207222v1_genomic.fna.gz | prodigal -d GCA_022072225.1_ASM2207222v1_genomic.fna/cds.fna -a GCA_022072225.1_ASM2207222v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-13 05:13:02,616] [INFO] Task succeeded: Prodigal
[2023-06-13 05:13:02,616] [INFO] Task started: HMMsearch
[2023-06-13 05:13:02,617] [INFO] Running command: hmmsearch --tblout GCA_022072225.1_ASM2207222v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6ea1ee76-cfe3-4f99-9b08-228bd7ffd9e3/dqc_reference/reference_markers.hmm GCA_022072225.1_ASM2207222v1_genomic.fna/protein.faa > /dev/null
[2023-06-13 05:13:02,913] [INFO] Task succeeded: HMMsearch
[2023-06-13 05:13:02,915] [INFO] Found 6/6 markers.
[2023-06-13 05:13:02,963] [INFO] Query marker FASTA was written to GCA_022072225.1_ASM2207222v1_genomic.fna/markers.fasta
[2023-06-13 05:13:02,964] [INFO] Task started: Blastn
[2023-06-13 05:13:02,964] [INFO] Running command: blastn -query GCA_022072225.1_ASM2207222v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6ea1ee76-cfe3-4f99-9b08-228bd7ffd9e3/dqc_reference/reference_markers.fasta -out GCA_022072225.1_ASM2207222v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 05:13:03,612] [INFO] Task succeeded: Blastn
[2023-06-13 05:13:03,620] [INFO] Selected 28 target genomes.
[2023-06-13 05:13:03,620] [INFO] Target genome list was writen to GCA_022072225.1_ASM2207222v1_genomic.fna/target_genomes.txt
[2023-06-13 05:13:03,627] [INFO] Task started: fastANI
[2023-06-13 05:13:03,627] [INFO] Running command: fastANI --query /var/lib/cwl/stgf839cd96-8488-4120-9114-04f0cddcc320/GCA_022072225.1_ASM2207222v1_genomic.fna.gz --refList GCA_022072225.1_ASM2207222v1_genomic.fna/target_genomes.txt --output GCA_022072225.1_ASM2207222v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-13 05:13:23,468] [INFO] Task succeeded: fastANI
[2023-06-13 05:13:23,468] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6ea1ee76-cfe3-4f99-9b08-228bd7ffd9e3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-13 05:13:23,469] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6ea1ee76-cfe3-4f99-9b08-228bd7ffd9e3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-13 05:13:23,471] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-13 05:13:23,471] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-13 05:13:23,471] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-13 05:13:23,474] [INFO] DFAST Taxonomy check result was written to GCA_022072225.1_ASM2207222v1_genomic.fna/tc_result.tsv
[2023-06-13 05:13:23,475] [INFO] ===== Taxonomy check completed =====
[2023-06-13 05:13:23,475] [INFO] ===== Start completeness check using CheckM =====
[2023-06-13 05:13:23,475] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6ea1ee76-cfe3-4f99-9b08-228bd7ffd9e3/dqc_reference/checkm_data
[2023-06-13 05:13:23,480] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-13 05:13:23,527] [INFO] Task started: CheckM
[2023-06-13 05:13:23,528] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_022072225.1_ASM2207222v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_022072225.1_ASM2207222v1_genomic.fna/checkm_input GCA_022072225.1_ASM2207222v1_genomic.fna/checkm_result
[2023-06-13 05:14:27,601] [INFO] Task succeeded: CheckM
[2023-06-13 05:14:27,603] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-13 05:14:27,625] [INFO] ===== Completeness check finished =====
[2023-06-13 05:14:27,625] [INFO] ===== Start GTDB Search =====
[2023-06-13 05:14:27,626] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_022072225.1_ASM2207222v1_genomic.fna/markers.fasta)
[2023-06-13 05:14:27,626] [INFO] Task started: Blastn
[2023-06-13 05:14:27,626] [INFO] Running command: blastn -query GCA_022072225.1_ASM2207222v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6ea1ee76-cfe3-4f99-9b08-228bd7ffd9e3/dqc_reference/reference_markers_gtdb.fasta -out GCA_022072225.1_ASM2207222v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 05:14:28,381] [INFO] Task succeeded: Blastn
[2023-06-13 05:14:28,386] [INFO] Selected 31 target genomes.
[2023-06-13 05:14:28,386] [INFO] Target genome list was writen to GCA_022072225.1_ASM2207222v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-13 05:14:28,405] [INFO] Task started: fastANI
[2023-06-13 05:14:28,405] [INFO] Running command: fastANI --query /var/lib/cwl/stgf839cd96-8488-4120-9114-04f0cddcc320/GCA_022072225.1_ASM2207222v1_genomic.fna.gz --refList GCA_022072225.1_ASM2207222v1_genomic.fna/target_genomes_gtdb.txt --output GCA_022072225.1_ASM2207222v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-13 05:14:47,574] [INFO] Task succeeded: fastANI
[2023-06-13 05:14:47,579] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-13 05:14:47,580] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_005773695.1	s__SXHM01 sp005773695	82.2575	321	1596	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__AKYH767;f__SXHM01;g__SXHM01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-13 05:14:47,582] [INFO] GTDB search result was written to GCA_022072225.1_ASM2207222v1_genomic.fna/result_gtdb.tsv
[2023-06-13 05:14:47,583] [INFO] ===== GTDB Search completed =====
[2023-06-13 05:14:47,585] [INFO] DFAST_QC result json was written to GCA_022072225.1_ASM2207222v1_genomic.fna/dqc_result.json
[2023-06-13 05:14:47,585] [INFO] DFAST_QC completed!
[2023-06-13 05:14:47,585] [INFO] Total running time: 0h2m9s