[2023-06-12 22:45:03,001] [INFO] DFAST_QC pipeline started.
[2023-06-12 22:45:03,003] [INFO] DFAST_QC version: 0.5.7
[2023-06-12 22:45:03,003] [INFO] DQC Reference Directory: /var/lib/cwl/stga59bf790-0172-4521-a8d7-14cc17d68e52/dqc_reference
[2023-06-12 22:45:04,158] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-12 22:45:04,159] [INFO] Task started: Prodigal
[2023-06-12 22:45:04,159] [INFO] Running command: gunzip -c /var/lib/cwl/stgedc03ab5-6d82-4f2b-87b5-a1fc00bee76c/GCA_022563175.1_ASM2256317v1_genomic.fna.gz | prodigal -d GCA_022563175.1_ASM2256317v1_genomic.fna/cds.fna -a GCA_022563175.1_ASM2256317v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-12 22:45:09,306] [INFO] Task succeeded: Prodigal
[2023-06-12 22:45:09,307] [INFO] Task started: HMMsearch
[2023-06-12 22:45:09,307] [INFO] Running command: hmmsearch --tblout GCA_022563175.1_ASM2256317v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga59bf790-0172-4521-a8d7-14cc17d68e52/dqc_reference/reference_markers.hmm GCA_022563175.1_ASM2256317v1_genomic.fna/protein.faa > /dev/null
[2023-06-12 22:45:09,465] [INFO] Task succeeded: HMMsearch
[2023-06-12 22:45:09,466] [INFO] Found 6/6 markers.
[2023-06-12 22:45:09,489] [INFO] Query marker FASTA was written to GCA_022563175.1_ASM2256317v1_genomic.fna/markers.fasta
[2023-06-12 22:45:09,490] [INFO] Task started: Blastn
[2023-06-12 22:45:09,490] [INFO] Running command: blastn -query GCA_022563175.1_ASM2256317v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga59bf790-0172-4521-a8d7-14cc17d68e52/dqc_reference/reference_markers.fasta -out GCA_022563175.1_ASM2256317v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-12 22:45:10,035] [INFO] Task succeeded: Blastn
[2023-06-12 22:45:10,039] [INFO] Selected 17 target genomes.
[2023-06-12 22:45:10,039] [INFO] Target genome list was writen to GCA_022563175.1_ASM2256317v1_genomic.fna/target_genomes.txt
[2023-06-12 22:45:10,043] [INFO] Task started: fastANI
[2023-06-12 22:45:10,043] [INFO] Running command: fastANI --query /var/lib/cwl/stgedc03ab5-6d82-4f2b-87b5-a1fc00bee76c/GCA_022563175.1_ASM2256317v1_genomic.fna.gz --refList GCA_022563175.1_ASM2256317v1_genomic.fna/target_genomes.txt --output GCA_022563175.1_ASM2256317v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-12 22:45:17,652] [INFO] Task succeeded: fastANI
[2023-06-12 22:45:17,652] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga59bf790-0172-4521-a8d7-14cc17d68e52/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-12 22:45:17,653] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga59bf790-0172-4521-a8d7-14cc17d68e52/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-12 22:45:17,654] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-12 22:45:17,654] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-12 22:45:17,654] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-12 22:45:17,656] [INFO] DFAST Taxonomy check result was written to GCA_022563175.1_ASM2256317v1_genomic.fna/tc_result.tsv
[2023-06-12 22:45:17,657] [INFO] ===== Taxonomy check completed =====
[2023-06-12 22:45:17,657] [INFO] ===== Start completeness check using CheckM =====
[2023-06-12 22:45:17,657] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga59bf790-0172-4521-a8d7-14cc17d68e52/dqc_reference/checkm_data
[2023-06-12 22:45:17,660] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-12 22:45:17,686] [INFO] Task started: CheckM
[2023-06-12 22:45:17,686] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_022563175.1_ASM2256317v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_022563175.1_ASM2256317v1_genomic.fna/checkm_input GCA_022563175.1_ASM2256317v1_genomic.fna/checkm_result
[2023-06-12 22:45:39,432] [INFO] Task succeeded: CheckM
[2023-06-12 22:45:39,434] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-12 22:45:39,450] [INFO] ===== Completeness check finished =====
[2023-06-12 22:45:39,450] [INFO] ===== Start GTDB Search =====
[2023-06-12 22:45:39,450] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_022563175.1_ASM2256317v1_genomic.fna/markers.fasta)
[2023-06-12 22:45:39,451] [INFO] Task started: Blastn
[2023-06-12 22:45:39,451] [INFO] Running command: blastn -query GCA_022563175.1_ASM2256317v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga59bf790-0172-4521-a8d7-14cc17d68e52/dqc_reference/reference_markers_gtdb.fasta -out GCA_022563175.1_ASM2256317v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-12 22:45:40,209] [INFO] Task succeeded: Blastn
[2023-06-12 22:45:40,213] [INFO] Selected 17 target genomes.
[2023-06-12 22:45:40,213] [INFO] Target genome list was writen to GCA_022563175.1_ASM2256317v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-12 22:45:40,222] [INFO] Task started: fastANI
[2023-06-12 22:45:40,223] [INFO] Running command: fastANI --query /var/lib/cwl/stgedc03ab5-6d82-4f2b-87b5-a1fc00bee76c/GCA_022563175.1_ASM2256317v1_genomic.fna.gz --refList GCA_022563175.1_ASM2256317v1_genomic.fna/target_genomes_gtdb.txt --output GCA_022563175.1_ASM2256317v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-12 22:45:47,400] [INFO] Task succeeded: fastANI
[2023-06-12 22:45:47,403] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-12 22:45:47,404] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_004402915.1	s__SORT01 sp004402915	81.9905	354	830	d__Bacteria;p__Marinisomatota;c__SORT01;o__SORT01;f__SORT01;g__SORT01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_004402895.1	s__SORT01 sp004402895	81.9209	378	830	d__Bacteria;p__Marinisomatota;c__SORT01;o__SORT01;f__SORT01;g__SORT01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-12 22:45:47,405] [INFO] GTDB search result was written to GCA_022563175.1_ASM2256317v1_genomic.fna/result_gtdb.tsv
[2023-06-12 22:45:47,406] [INFO] ===== GTDB Search completed =====
[2023-06-12 22:45:47,408] [INFO] DFAST_QC result json was written to GCA_022563175.1_ASM2256317v1_genomic.fna/dqc_result.json
[2023-06-12 22:45:47,408] [INFO] DFAST_QC completed!
[2023-06-12 22:45:47,408] [INFO] Total running time: 0h0m44s