[2023-06-13 03:34:35,455] [INFO] DFAST_QC pipeline started.
[2023-06-13 03:34:35,461] [INFO] DFAST_QC version: 0.5.7
[2023-06-13 03:34:35,461] [INFO] DQC Reference Directory: /var/lib/cwl/stg8e88d09d-2e28-4b22-842d-7e54ab2def69/dqc_reference
[2023-06-13 03:34:36,895] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-13 03:34:36,897] [INFO] Task started: Prodigal
[2023-06-13 03:34:36,898] [INFO] Running command: gunzip -c /var/lib/cwl/stgbcda4ee7-4b67-444b-8c89-8f47a68bfcae/GCA_022564895.1_ASM2256489v1_genomic.fna.gz | prodigal -d GCA_022564895.1_ASM2256489v1_genomic.fna/cds.fna -a GCA_022564895.1_ASM2256489v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-13 03:34:46,459] [INFO] Task succeeded: Prodigal
[2023-06-13 03:34:46,459] [INFO] Task started: HMMsearch
[2023-06-13 03:34:46,459] [INFO] Running command: hmmsearch --tblout GCA_022564895.1_ASM2256489v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8e88d09d-2e28-4b22-842d-7e54ab2def69/dqc_reference/reference_markers.hmm GCA_022564895.1_ASM2256489v1_genomic.fna/protein.faa > /dev/null
[2023-06-13 03:34:46,720] [INFO] Task succeeded: HMMsearch
[2023-06-13 03:34:46,721] [INFO] Found 6/6 markers.
[2023-06-13 03:34:46,753] [INFO] Query marker FASTA was written to GCA_022564895.1_ASM2256489v1_genomic.fna/markers.fasta
[2023-06-13 03:34:46,753] [INFO] Task started: Blastn
[2023-06-13 03:34:46,753] [INFO] Running command: blastn -query GCA_022564895.1_ASM2256489v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg8e88d09d-2e28-4b22-842d-7e54ab2def69/dqc_reference/reference_markers.fasta -out GCA_022564895.1_ASM2256489v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 03:34:47,338] [INFO] Task succeeded: Blastn
[2023-06-13 03:34:47,342] [INFO] Selected 19 target genomes.
[2023-06-13 03:34:47,342] [INFO] Target genome list was writen to GCA_022564895.1_ASM2256489v1_genomic.fna/target_genomes.txt
[2023-06-13 03:34:47,347] [INFO] Task started: fastANI
[2023-06-13 03:34:47,347] [INFO] Running command: fastANI --query /var/lib/cwl/stgbcda4ee7-4b67-444b-8c89-8f47a68bfcae/GCA_022564895.1_ASM2256489v1_genomic.fna.gz --refList GCA_022564895.1_ASM2256489v1_genomic.fna/target_genomes.txt --output GCA_022564895.1_ASM2256489v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-13 03:35:00,849] [INFO] Task succeeded: fastANI
[2023-06-13 03:35:00,849] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8e88d09d-2e28-4b22-842d-7e54ab2def69/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-13 03:35:00,850] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8e88d09d-2e28-4b22-842d-7e54ab2def69/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-13 03:35:00,852] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-13 03:35:00,852] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-13 03:35:00,852] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-13 03:35:00,855] [INFO] DFAST Taxonomy check result was written to GCA_022564895.1_ASM2256489v1_genomic.fna/tc_result.tsv
[2023-06-13 03:35:00,855] [INFO] ===== Taxonomy check completed =====
[2023-06-13 03:35:00,855] [INFO] ===== Start completeness check using CheckM =====
[2023-06-13 03:35:00,856] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8e88d09d-2e28-4b22-842d-7e54ab2def69/dqc_reference/checkm_data
[2023-06-13 03:35:00,859] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-13 03:35:00,901] [INFO] Task started: CheckM
[2023-06-13 03:35:00,901] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_022564895.1_ASM2256489v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_022564895.1_ASM2256489v1_genomic.fna/checkm_input GCA_022564895.1_ASM2256489v1_genomic.fna/checkm_result
[2023-06-13 03:35:34,381] [INFO] Task succeeded: CheckM
[2023-06-13 03:35:34,383] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 5.56%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-13 03:35:34,408] [INFO] ===== Completeness check finished =====
[2023-06-13 03:35:34,409] [INFO] ===== Start GTDB Search =====
[2023-06-13 03:35:34,409] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_022564895.1_ASM2256489v1_genomic.fna/markers.fasta)
[2023-06-13 03:35:34,409] [INFO] Task started: Blastn
[2023-06-13 03:35:34,410] [INFO] Running command: blastn -query GCA_022564895.1_ASM2256489v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg8e88d09d-2e28-4b22-842d-7e54ab2def69/dqc_reference/reference_markers_gtdb.fasta -out GCA_022564895.1_ASM2256489v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 03:35:35,196] [INFO] Task succeeded: Blastn
[2023-06-13 03:35:35,201] [INFO] Selected 29 target genomes.
[2023-06-13 03:35:35,202] [INFO] Target genome list was writen to GCA_022564895.1_ASM2256489v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-13 03:35:35,219] [INFO] Task started: fastANI
[2023-06-13 03:35:35,219] [INFO] Running command: fastANI --query /var/lib/cwl/stgbcda4ee7-4b67-444b-8c89-8f47a68bfcae/GCA_022564895.1_ASM2256489v1_genomic.fna.gz --refList GCA_022564895.1_ASM2256489v1_genomic.fna/target_genomes_gtdb.txt --output GCA_022564895.1_ASM2256489v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-13 03:35:48,181] [INFO] Task succeeded: fastANI
[2023-06-13 03:35:48,183] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-13 03:35:48,184] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-06-13 03:35:48,186] [INFO] GTDB search result was written to GCA_022564895.1_ASM2256489v1_genomic.fna/result_gtdb.tsv
[2023-06-13 03:35:48,187] [INFO] ===== GTDB Search completed =====
[2023-06-13 03:35:48,189] [INFO] DFAST_QC result json was written to GCA_022564895.1_ASM2256489v1_genomic.fna/dqc_result.json
[2023-06-13 03:35:48,190] [INFO] DFAST_QC completed!
[2023-06-13 03:35:48,190] [INFO] Total running time: 0h1m13s