[2023-06-13 22:42:40,374] [INFO] DFAST_QC pipeline started.
[2023-06-13 22:42:40,375] [INFO] DFAST_QC version: 0.5.7
[2023-06-13 22:42:40,375] [INFO] DQC Reference Directory: /var/lib/cwl/stg32e7fc16-56af-46cd-ae22-cecf367461cd/dqc_reference
[2023-06-13 22:42:41,529] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-13 22:42:41,530] [INFO] Task started: Prodigal
[2023-06-13 22:42:41,530] [INFO] Running command: gunzip -c /var/lib/cwl/stg82327254-5bd4-4369-b62d-5ec73895a9e3/GCA_022565115.1_ASM2256511v1_genomic.fna.gz | prodigal -d GCA_022565115.1_ASM2256511v1_genomic.fna/cds.fna -a GCA_022565115.1_ASM2256511v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-13 22:42:43,211] [INFO] Task succeeded: Prodigal
[2023-06-13 22:42:43,211] [INFO] Task started: HMMsearch
[2023-06-13 22:42:43,211] [INFO] Running command: hmmsearch --tblout GCA_022565115.1_ASM2256511v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg32e7fc16-56af-46cd-ae22-cecf367461cd/dqc_reference/reference_markers.hmm GCA_022565115.1_ASM2256511v1_genomic.fna/protein.faa > /dev/null
[2023-06-13 22:42:43,346] [INFO] Task succeeded: HMMsearch
[2023-06-13 22:42:43,347] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg82327254-5bd4-4369-b62d-5ec73895a9e3/GCA_022565115.1_ASM2256511v1_genomic.fna.gz]
[2023-06-13 22:42:43,357] [INFO] Query marker FASTA was written to GCA_022565115.1_ASM2256511v1_genomic.fna/markers.fasta
[2023-06-13 22:42:43,357] [INFO] Task started: Blastn
[2023-06-13 22:42:43,357] [INFO] Running command: blastn -query GCA_022565115.1_ASM2256511v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg32e7fc16-56af-46cd-ae22-cecf367461cd/dqc_reference/reference_markers.fasta -out GCA_022565115.1_ASM2256511v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 22:42:43,843] [INFO] Task succeeded: Blastn
[2023-06-13 22:42:43,846] [INFO] Selected 12 target genomes.
[2023-06-13 22:42:43,846] [INFO] Target genome list was writen to GCA_022565115.1_ASM2256511v1_genomic.fna/target_genomes.txt
[2023-06-13 22:42:43,849] [INFO] Task started: fastANI
[2023-06-13 22:42:43,849] [INFO] Running command: fastANI --query /var/lib/cwl/stg82327254-5bd4-4369-b62d-5ec73895a9e3/GCA_022565115.1_ASM2256511v1_genomic.fna.gz --refList GCA_022565115.1_ASM2256511v1_genomic.fna/target_genomes.txt --output GCA_022565115.1_ASM2256511v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-13 22:42:49,807] [INFO] Task succeeded: fastANI
[2023-06-13 22:42:49,807] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg32e7fc16-56af-46cd-ae22-cecf367461cd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-13 22:42:49,808] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg32e7fc16-56af-46cd-ae22-cecf367461cd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-13 22:42:49,809] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-13 22:42:49,809] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-13 22:42:49,809] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-13 22:42:49,811] [INFO] DFAST Taxonomy check result was written to GCA_022565115.1_ASM2256511v1_genomic.fna/tc_result.tsv
[2023-06-13 22:42:49,811] [INFO] ===== Taxonomy check completed =====
[2023-06-13 22:42:49,811] [INFO] ===== Start completeness check using CheckM =====
[2023-06-13 22:42:49,812] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg32e7fc16-56af-46cd-ae22-cecf367461cd/dqc_reference/checkm_data
[2023-06-13 22:42:49,814] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-13 22:42:49,822] [INFO] Task started: CheckM
[2023-06-13 22:42:49,822] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_022565115.1_ASM2256511v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_022565115.1_ASM2256511v1_genomic.fna/checkm_input GCA_022565115.1_ASM2256511v1_genomic.fna/checkm_result
[2023-06-13 22:43:01,504] [INFO] Task succeeded: CheckM
[2023-06-13 22:43:01,505] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 65.21%
Contamintation: 9.85%
Strain heterogeneity: 16.67%
--------------------------------------------------------------------------------
[2023-06-13 22:43:01,530] [INFO] ===== Completeness check finished =====
[2023-06-13 22:43:01,530] [INFO] ===== Start GTDB Search =====
[2023-06-13 22:43:01,530] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_022565115.1_ASM2256511v1_genomic.fna/markers.fasta)
[2023-06-13 22:43:01,531] [INFO] Task started: Blastn
[2023-06-13 22:43:01,531] [INFO] Running command: blastn -query GCA_022565115.1_ASM2256511v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg32e7fc16-56af-46cd-ae22-cecf367461cd/dqc_reference/reference_markers_gtdb.fasta -out GCA_022565115.1_ASM2256511v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 22:43:02,138] [INFO] Task succeeded: Blastn
[2023-06-13 22:43:02,141] [INFO] Selected 15 target genomes.
[2023-06-13 22:43:02,141] [INFO] Target genome list was writen to GCA_022565115.1_ASM2256511v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-13 22:43:02,191] [INFO] Task started: fastANI
[2023-06-13 22:43:02,191] [INFO] Running command: fastANI --query /var/lib/cwl/stg82327254-5bd4-4369-b62d-5ec73895a9e3/GCA_022565115.1_ASM2256511v1_genomic.fna.gz --refList GCA_022565115.1_ASM2256511v1_genomic.fna/target_genomes_gtdb.txt --output GCA_022565115.1_ASM2256511v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-13 22:43:04,860] [INFO] Task succeeded: fastANI
[2023-06-13 22:43:04,861] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-13 22:43:04,862] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-06-13 22:43:04,863] [INFO] GTDB search result was written to GCA_022565115.1_ASM2256511v1_genomic.fna/result_gtdb.tsv
[2023-06-13 22:43:04,864] [INFO] ===== GTDB Search completed =====
[2023-06-13 22:43:04,866] [INFO] DFAST_QC result json was written to GCA_022565115.1_ASM2256511v1_genomic.fna/dqc_result.json
[2023-06-13 22:43:04,866] [INFO] DFAST_QC completed!
[2023-06-13 22:43:04,866] [INFO] Total running time: 0h0m24s