[2023-06-13 02:32:37,833] [INFO] DFAST_QC pipeline started.
[2023-06-13 02:32:37,839] [INFO] DFAST_QC version: 0.5.7
[2023-06-13 02:32:37,840] [INFO] DQC Reference Directory: /var/lib/cwl/stga1c4cc63-c097-4e17-8d33-3b7398605d13/dqc_reference
[2023-06-13 02:32:39,720] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-13 02:32:39,721] [INFO] Task started: Prodigal
[2023-06-13 02:32:39,722] [INFO] Running command: gunzip -c /var/lib/cwl/stgbcf50d5a-f2ce-4e54-8930-180b851a0b98/GCA_022708325.1_ASM2270832v1_genomic.fna.gz | prodigal -d GCA_022708325.1_ASM2270832v1_genomic.fna/cds.fna -a GCA_022708325.1_ASM2270832v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-13 02:32:52,683] [INFO] Task succeeded: Prodigal
[2023-06-13 02:32:52,684] [INFO] Task started: HMMsearch
[2023-06-13 02:32:52,684] [INFO] Running command: hmmsearch --tblout GCA_022708325.1_ASM2270832v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga1c4cc63-c097-4e17-8d33-3b7398605d13/dqc_reference/reference_markers.hmm GCA_022708325.1_ASM2270832v1_genomic.fna/protein.faa > /dev/null
[2023-06-13 02:32:52,913] [INFO] Task succeeded: HMMsearch
[2023-06-13 02:32:52,914] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgbcf50d5a-f2ce-4e54-8930-180b851a0b98/GCA_022708325.1_ASM2270832v1_genomic.fna.gz]
[2023-06-13 02:32:52,939] [INFO] Query marker FASTA was written to GCA_022708325.1_ASM2270832v1_genomic.fna/markers.fasta
[2023-06-13 02:32:52,939] [INFO] Task started: Blastn
[2023-06-13 02:32:52,939] [INFO] Running command: blastn -query GCA_022708325.1_ASM2270832v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga1c4cc63-c097-4e17-8d33-3b7398605d13/dqc_reference/reference_markers.fasta -out GCA_022708325.1_ASM2270832v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 02:32:53,586] [INFO] Task succeeded: Blastn
[2023-06-13 02:32:53,590] [INFO] Selected 24 target genomes.
[2023-06-13 02:32:53,590] [INFO] Target genome list was writen to GCA_022708325.1_ASM2270832v1_genomic.fna/target_genomes.txt
[2023-06-13 02:32:53,594] [INFO] Task started: fastANI
[2023-06-13 02:32:53,594] [INFO] Running command: fastANI --query /var/lib/cwl/stgbcf50d5a-f2ce-4e54-8930-180b851a0b98/GCA_022708325.1_ASM2270832v1_genomic.fna.gz --refList GCA_022708325.1_ASM2270832v1_genomic.fna/target_genomes.txt --output GCA_022708325.1_ASM2270832v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-13 02:33:08,233] [INFO] Task succeeded: fastANI
[2023-06-13 02:33:08,233] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga1c4cc63-c097-4e17-8d33-3b7398605d13/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-13 02:33:08,234] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga1c4cc63-c097-4e17-8d33-3b7398605d13/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-13 02:33:08,235] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-13 02:33:08,235] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-13 02:33:08,235] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-13 02:33:08,240] [INFO] DFAST Taxonomy check result was written to GCA_022708325.1_ASM2270832v1_genomic.fna/tc_result.tsv
[2023-06-13 02:33:08,241] [INFO] ===== Taxonomy check completed =====
[2023-06-13 02:33:08,242] [INFO] ===== Start completeness check using CheckM =====
[2023-06-13 02:33:08,242] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga1c4cc63-c097-4e17-8d33-3b7398605d13/dqc_reference/checkm_data
[2023-06-13 02:33:08,245] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-13 02:33:08,274] [INFO] Task started: CheckM
[2023-06-13 02:33:08,274] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_022708325.1_ASM2270832v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_022708325.1_ASM2270832v1_genomic.fna/checkm_input GCA_022708325.1_ASM2270832v1_genomic.fna/checkm_result
[2023-06-13 02:33:49,730] [INFO] Task succeeded: CheckM
[2023-06-13 02:33:49,731] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 0.93%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-13 02:33:49,754] [INFO] ===== Completeness check finished =====
[2023-06-13 02:33:49,754] [INFO] ===== Start GTDB Search =====
[2023-06-13 02:33:49,755] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_022708325.1_ASM2270832v1_genomic.fna/markers.fasta)
[2023-06-13 02:33:49,755] [INFO] Task started: Blastn
[2023-06-13 02:33:49,755] [INFO] Running command: blastn -query GCA_022708325.1_ASM2270832v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga1c4cc63-c097-4e17-8d33-3b7398605d13/dqc_reference/reference_markers_gtdb.fasta -out GCA_022708325.1_ASM2270832v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 02:33:50,842] [INFO] Task succeeded: Blastn
[2023-06-13 02:33:50,847] [INFO] Selected 20 target genomes.
[2023-06-13 02:33:50,847] [INFO] Target genome list was writen to GCA_022708325.1_ASM2270832v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-13 02:33:50,875] [INFO] Task started: fastANI
[2023-06-13 02:33:50,875] [INFO] Running command: fastANI --query /var/lib/cwl/stgbcf50d5a-f2ce-4e54-8930-180b851a0b98/GCA_022708325.1_ASM2270832v1_genomic.fna.gz --refList GCA_022708325.1_ASM2270832v1_genomic.fna/target_genomes_gtdb.txt --output GCA_022708325.1_ASM2270832v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-13 02:34:01,067] [INFO] Task succeeded: fastANI
[2023-06-13 02:34:01,074] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-13 02:34:01,074] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_003506275.1	s__UBA8389 sp003506275	97.2574	424	554	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA1402;g__UBA8389	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_012718945.1	s__UBA8389 sp012718945	83.2348	353	554	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA1402;g__UBA8389	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-13 02:34:01,077] [INFO] GTDB search result was written to GCA_022708325.1_ASM2270832v1_genomic.fna/result_gtdb.tsv
[2023-06-13 02:34:01,077] [INFO] ===== GTDB Search completed =====
[2023-06-13 02:34:01,080] [INFO] DFAST_QC result json was written to GCA_022708325.1_ASM2270832v1_genomic.fna/dqc_result.json
[2023-06-13 02:34:01,080] [INFO] DFAST_QC completed!
[2023-06-13 02:34:01,080] [INFO] Total running time: 0h1m23s