[2023-06-13 18:12:17,684] [INFO] DFAST_QC pipeline started.
[2023-06-13 18:12:17,686] [INFO] DFAST_QC version: 0.5.7
[2023-06-13 18:12:17,686] [INFO] DQC Reference Directory: /var/lib/cwl/stgb6feefe8-7469-4d3f-a29f-0bbebc56e19f/dqc_reference
[2023-06-13 18:12:19,743] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-13 18:12:19,744] [INFO] Task started: Prodigal
[2023-06-13 18:12:19,744] [INFO] Running command: gunzip -c /var/lib/cwl/stgb191687b-41af-4589-be7f-35ed83745e23/GCA_022708785.1_ASM2270878v1_genomic.fna.gz | prodigal -d GCA_022708785.1_ASM2270878v1_genomic.fna/cds.fna -a GCA_022708785.1_ASM2270878v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-13 18:12:22,583] [INFO] Task succeeded: Prodigal
[2023-06-13 18:12:22,583] [INFO] Task started: HMMsearch
[2023-06-13 18:12:22,584] [INFO] Running command: hmmsearch --tblout GCA_022708785.1_ASM2270878v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb6feefe8-7469-4d3f-a29f-0bbebc56e19f/dqc_reference/reference_markers.hmm GCA_022708785.1_ASM2270878v1_genomic.fna/protein.faa > /dev/null
[2023-06-13 18:12:22,728] [INFO] Task succeeded: HMMsearch
[2023-06-13 18:12:22,729] [WARNING] Found 4/6 markers. [/var/lib/cwl/stgb191687b-41af-4589-be7f-35ed83745e23/GCA_022708785.1_ASM2270878v1_genomic.fna.gz]
[2023-06-13 18:12:22,750] [INFO] Query marker FASTA was written to GCA_022708785.1_ASM2270878v1_genomic.fna/markers.fasta
[2023-06-13 18:12:22,750] [INFO] Task started: Blastn
[2023-06-13 18:12:22,750] [INFO] Running command: blastn -query GCA_022708785.1_ASM2270878v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb6feefe8-7469-4d3f-a29f-0bbebc56e19f/dqc_reference/reference_markers.fasta -out GCA_022708785.1_ASM2270878v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 18:12:24,141] [INFO] Task succeeded: Blastn
[2023-06-13 18:12:24,144] [INFO] Selected 10 target genomes.
[2023-06-13 18:12:24,144] [INFO] Target genome list was writen to GCA_022708785.1_ASM2270878v1_genomic.fna/target_genomes.txt
[2023-06-13 18:12:24,169] [INFO] Task started: fastANI
[2023-06-13 18:12:24,169] [INFO] Running command: fastANI --query /var/lib/cwl/stgb191687b-41af-4589-be7f-35ed83745e23/GCA_022708785.1_ASM2270878v1_genomic.fna.gz --refList GCA_022708785.1_ASM2270878v1_genomic.fna/target_genomes.txt --output GCA_022708785.1_ASM2270878v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-13 18:12:28,739] [INFO] Task succeeded: fastANI
[2023-06-13 18:12:28,739] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb6feefe8-7469-4d3f-a29f-0bbebc56e19f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-13 18:12:28,740] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb6feefe8-7469-4d3f-a29f-0bbebc56e19f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-13 18:12:28,741] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-13 18:12:28,741] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-13 18:12:28,741] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-13 18:12:28,743] [INFO] DFAST Taxonomy check result was written to GCA_022708785.1_ASM2270878v1_genomic.fna/tc_result.tsv
[2023-06-13 18:12:28,744] [INFO] ===== Taxonomy check completed =====
[2023-06-13 18:12:28,744] [INFO] ===== Start completeness check using CheckM =====
[2023-06-13 18:12:28,744] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb6feefe8-7469-4d3f-a29f-0bbebc56e19f/dqc_reference/checkm_data
[2023-06-13 18:12:28,746] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-13 18:12:28,765] [INFO] Task started: CheckM
[2023-06-13 18:12:28,765] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_022708785.1_ASM2270878v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_022708785.1_ASM2270878v1_genomic.fna/checkm_input GCA_022708785.1_ASM2270878v1_genomic.fna/checkm_result
[2023-06-13 18:12:45,085] [INFO] Task succeeded: CheckM
[2023-06-13 18:12:45,086] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 86.57%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-13 18:12:45,107] [INFO] ===== Completeness check finished =====
[2023-06-13 18:12:45,108] [INFO] ===== Start GTDB Search =====
[2023-06-13 18:12:45,108] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_022708785.1_ASM2270878v1_genomic.fna/markers.fasta)
[2023-06-13 18:12:45,108] [INFO] Task started: Blastn
[2023-06-13 18:12:45,108] [INFO] Running command: blastn -query GCA_022708785.1_ASM2270878v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb6feefe8-7469-4d3f-a29f-0bbebc56e19f/dqc_reference/reference_markers_gtdb.fasta -out GCA_022708785.1_ASM2270878v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-13 18:12:45,563] [INFO] Task succeeded: Blastn
[2023-06-13 18:12:45,569] [INFO] Selected 13 target genomes.
[2023-06-13 18:12:45,569] [INFO] Target genome list was writen to GCA_022708785.1_ASM2270878v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-13 18:12:45,577] [INFO] Task started: fastANI
[2023-06-13 18:12:45,577] [INFO] Running command: fastANI --query /var/lib/cwl/stgb191687b-41af-4589-be7f-35ed83745e23/GCA_022708785.1_ASM2270878v1_genomic.fna.gz --refList GCA_022708785.1_ASM2270878v1_genomic.fna/target_genomes_gtdb.txt --output GCA_022708785.1_ASM2270878v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-13 18:12:50,170] [INFO] Task succeeded: fastANI
[2023-06-13 18:12:50,176] [INFO] Found 3 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-13 18:12:50,176] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002068775.1	s__Fen731 sp002068775	99.1906	286	427	d__Bacteria;p__Actinobacteriota;c__Humimicrobiia;o__Humimicrobiales;f__Humimicrobiaceae;g__Fen731	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_016927635.1	s__VGAZ01 sp016927635	76.8437	50	427	d__Bacteria;p__Actinobacteriota;c__Humimicrobiia;o__Humimicrobiales;f__Humimicrobiaceae;g__VGAZ01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016870615.1	s__VGAZ01 sp016870615	76.827	57	427	d__Bacteria;p__Actinobacteriota;c__Humimicrobiia;o__Humimicrobiales;f__Humimicrobiaceae;g__VGAZ01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-13 18:12:50,179] [INFO] GTDB search result was written to GCA_022708785.1_ASM2270878v1_genomic.fna/result_gtdb.tsv
[2023-06-13 18:12:50,180] [INFO] ===== GTDB Search completed =====
[2023-06-13 18:12:50,182] [INFO] DFAST_QC result json was written to GCA_022708785.1_ASM2270878v1_genomic.fna/dqc_result.json
[2023-06-13 18:12:50,183] [INFO] DFAST_QC completed!
[2023-06-13 18:12:50,183] [INFO] Total running time: 0h0m32s