[2023-06-29 18:37:30,197] [INFO] DFAST_QC pipeline started.
[2023-06-29 18:37:30,200] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 18:37:30,200] [INFO] DQC Reference Directory: /var/lib/cwl/stgfa6562e1-580a-408b-b531-f18bb19ae52b/dqc_reference
[2023-06-29 18:37:31,405] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 18:37:31,406] [INFO] Task started: Prodigal
[2023-06-29 18:37:31,406] [INFO] Running command: gunzip -c /var/lib/cwl/stg320d15ab-8fa4-4229-9559-59137356fde0/GCA_023708445.1_ASM2370844v1_genomic.fna.gz | prodigal -d GCA_023708445.1_ASM2370844v1_genomic.fna/cds.fna -a GCA_023708445.1_ASM2370844v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 18:37:39,340] [INFO] Task succeeded: Prodigal
[2023-06-29 18:37:39,341] [INFO] Task started: HMMsearch
[2023-06-29 18:37:39,341] [INFO] Running command: hmmsearch --tblout GCA_023708445.1_ASM2370844v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfa6562e1-580a-408b-b531-f18bb19ae52b/dqc_reference/reference_markers.hmm GCA_023708445.1_ASM2370844v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 18:37:39,603] [INFO] Task succeeded: HMMsearch
[2023-06-29 18:37:39,604] [INFO] Found 6/6 markers.
[2023-06-29 18:37:39,655] [INFO] Query marker FASTA was written to GCA_023708445.1_ASM2370844v1_genomic.fna/markers.fasta
[2023-06-29 18:37:39,655] [INFO] Task started: Blastn
[2023-06-29 18:37:39,656] [INFO] Running command: blastn -query GCA_023708445.1_ASM2370844v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgfa6562e1-580a-408b-b531-f18bb19ae52b/dqc_reference/reference_markers.fasta -out GCA_023708445.1_ASM2370844v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 18:37:40,617] [INFO] Task succeeded: Blastn
[2023-06-29 18:37:40,621] [INFO] Selected 14 target genomes.
[2023-06-29 18:37:40,621] [INFO] Target genome list was writen to GCA_023708445.1_ASM2370844v1_genomic.fna/target_genomes.txt
[2023-06-29 18:37:40,624] [INFO] Task started: fastANI
[2023-06-29 18:37:40,624] [INFO] Running command: fastANI --query /var/lib/cwl/stg320d15ab-8fa4-4229-9559-59137356fde0/GCA_023708445.1_ASM2370844v1_genomic.fna.gz --refList GCA_023708445.1_ASM2370844v1_genomic.fna/target_genomes.txt --output GCA_023708445.1_ASM2370844v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 18:37:48,077] [INFO] Task succeeded: fastANI
[2023-06-29 18:37:48,078] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgfa6562e1-580a-408b-b531-f18bb19ae52b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 18:37:48,078] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgfa6562e1-580a-408b-b531-f18bb19ae52b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 18:37:48,090] [INFO] Found 13 fastANI hits (2 hits with ANI > threshold)
[2023-06-29 18:37:48,090] [INFO] The taxonomy check result is classified as 'conclusive'.
[2023-06-29 18:37:48,090] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Bifidobacterium adolescentis	strain=NCTC11814	GCA_900445615.1	1680	1680	type	True	97.7166	595	747	95	conclusive
Bifidobacterium adolescentis	strain=ATCC 15703	GCA_000010425.1	1680	1680	type	True	97.7089	594	747	95	conclusive
Bifidobacterium ruminantium	strain=DSM 6489	GCA_000770925.1	78346	78346	type	True	88.9164	528	747	95	below_threshold
Bifidobacterium ruminantium	strain=DSM 6489	GCA_000687635.1	78346	78346	type	True	88.9033	540	747	95	below_threshold
Bifidobacterium catenulatum	strain=DSM 16992	GCA_000771025.1	1686	1686	type	True	84.6045	451	747	95	below_threshold
Bifidobacterium pseudocatenulatum	strain=DSM 20438	GCA_000771445.1	28026	28026	type	True	84.4762	491	747	95	below_threshold
Bifidobacterium catenulatum subsp. kashiwanohense	strain=JCM 15439	GCA_001042615.1	630129	1686	type	True	84.4376	481	747	95	below_threshold
Bifidobacterium catenulatum subsp. kashiwanohense	strain=DSM 21854	GCA_000771545.1	630129	1686	type	True	84.3812	477	747	95	below_threshold
Bifidobacterium catenulatum subsp. kashiwanohense	strain=DSM 21854	GCA_000741605.1	630129	1686	type	True	84.379	481	747	95	below_threshold
Bifidobacterium moukalabense	strain=DSM 27321	GCA_000522505.1	1333651	1333651	type	True	83.4802	491	747	95	below_threshold
Bifidobacterium dentium	strain=JCM 1195	GCA_001042595.1	1689	1689	type	True	83.0285	502	747	95	below_threshold
Bifidobacterium amazonense	strain=MA1	GCA_018555435.2	2809027	2809027	type	True	81.191	382	747	95	below_threshold
Bifidobacterium pseudolongum subsp. pseudolongum	strain=ATCC 25526	GCA_022691205.1	31954	1694	type	True	79.4643	252	747	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-29 18:37:48,092] [INFO] DFAST Taxonomy check result was written to GCA_023708445.1_ASM2370844v1_genomic.fna/tc_result.tsv
[2023-06-29 18:37:48,093] [INFO] ===== Taxonomy check completed =====
[2023-06-29 18:37:48,093] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 18:37:48,094] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfa6562e1-580a-408b-b531-f18bb19ae52b/dqc_reference/checkm_data
[2023-06-29 18:37:48,095] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 18:37:48,120] [INFO] Task started: CheckM
[2023-06-29 18:37:48,121] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_023708445.1_ASM2370844v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_023708445.1_ASM2370844v1_genomic.fna/checkm_input GCA_023708445.1_ASM2370844v1_genomic.fna/checkm_result
[2023-06-29 18:38:17,047] [INFO] Task succeeded: CheckM
[2023-06-29 18:38:17,049] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 18:38:17,073] [INFO] ===== Completeness check finished =====
[2023-06-29 18:38:17,073] [INFO] ===== Start GTDB Search =====
[2023-06-29 18:38:17,074] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_023708445.1_ASM2370844v1_genomic.fna/markers.fasta)
[2023-06-29 18:38:17,074] [INFO] Task started: Blastn
[2023-06-29 18:38:17,074] [INFO] Running command: blastn -query GCA_023708445.1_ASM2370844v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgfa6562e1-580a-408b-b531-f18bb19ae52b/dqc_reference/reference_markers_gtdb.fasta -out GCA_023708445.1_ASM2370844v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 18:38:18,260] [INFO] Task succeeded: Blastn
[2023-06-29 18:38:18,265] [INFO] Selected 10 target genomes.
[2023-06-29 18:38:18,265] [INFO] Target genome list was writen to GCA_023708445.1_ASM2370844v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 18:38:18,269] [INFO] Task started: fastANI
[2023-06-29 18:38:18,269] [INFO] Running command: fastANI --query /var/lib/cwl/stg320d15ab-8fa4-4229-9559-59137356fde0/GCA_023708445.1_ASM2370844v1_genomic.fna.gz --refList GCA_023708445.1_ASM2370844v1_genomic.fna/target_genomes_gtdb.txt --output GCA_023708445.1_ASM2370844v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 18:38:23,359] [INFO] Task succeeded: fastANI
[2023-06-29 18:38:23,371] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-29 18:38:23,371] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000010425.1	s__Bifidobacterium adolescentis	97.7089	594	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.09	95.61	0.87	0.77	128	conclusive
GCF_000770925.1	s__Bifidobacterium ruminantium	88.8933	529	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.91	97.77	0.92	0.85	6	-
GCF_001025195.1	s__Bifidobacterium catenulatum	84.6588	469	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	96.3948	99.04	98.03	0.94	0.87	12	-
GCF_001025215.1	s__Bifidobacterium pseudocatenulatum	84.6181	491	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.12	96.90	0.90	0.83	112	-
GCF_002742445.1	s__Bifidobacterium sp002742445	84.5438	459	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	97.43	96.66	0.87	0.84	9	-
GCF_001042615.1	s__Bifidobacterium kashiwanohense	84.4463	480	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	96.3948	99.07	97.92	0.95	0.91	5	-
GCF_000800455.1	s__Bifidobacterium kashiwanohense_A	84.1994	476	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.1942	N/A	N/A	N/A	N/A	1	-
GCA_002451435.1	s__Bifidobacterium sp002451435	84.0312	413	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	99.30	98.61	0.87	0.84	20	-
GCF_000522505.1	s__Bifidobacterium moukalabense	83.4799	491	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	99.37	98.94	0.93	0.88	13	-
GCF_001042595.1	s__Bifidobacterium dentium	83.0285	502	747	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.95	98.47	0.92	0.86	50	-
--------------------------------------------------------------------------------
[2023-06-29 18:38:23,374] [INFO] GTDB search result was written to GCA_023708445.1_ASM2370844v1_genomic.fna/result_gtdb.tsv
[2023-06-29 18:38:23,375] [INFO] ===== GTDB Search completed =====
[2023-06-29 18:38:23,381] [INFO] DFAST_QC result json was written to GCA_023708445.1_ASM2370844v1_genomic.fna/dqc_result.json
[2023-06-29 18:38:23,382] [INFO] DFAST_QC completed!
[2023-06-29 18:38:23,382] [INFO] Total running time: 0h0m53s