[2023-06-29 12:50:35,108] [INFO] DFAST_QC pipeline started.
[2023-06-29 12:50:35,111] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 12:50:35,111] [INFO] DQC Reference Directory: /var/lib/cwl/stg26b6091d-369d-4f28-93ed-6a8a8b209686/dqc_reference
[2023-06-29 12:50:36,358] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 12:50:36,359] [INFO] Task started: Prodigal
[2023-06-29 12:50:36,359] [INFO] Running command: gunzip -c /var/lib/cwl/stg9d233ed9-c0ba-4a0d-a03c-eae69da6186d/GCA_023819285.1_ASM2381928v1_genomic.fna.gz | prodigal -d GCA_023819285.1_ASM2381928v1_genomic.fna/cds.fna -a GCA_023819285.1_ASM2381928v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 12:50:38,931] [INFO] Task succeeded: Prodigal
[2023-06-29 12:50:38,932] [INFO] Task started: HMMsearch
[2023-06-29 12:50:38,932] [INFO] Running command: hmmsearch --tblout GCA_023819285.1_ASM2381928v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg26b6091d-369d-4f28-93ed-6a8a8b209686/dqc_reference/reference_markers.hmm GCA_023819285.1_ASM2381928v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 12:50:39,166] [INFO] Task succeeded: HMMsearch
[2023-06-29 12:50:39,167] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg9d233ed9-c0ba-4a0d-a03c-eae69da6186d/GCA_023819285.1_ASM2381928v1_genomic.fna.gz]
[2023-06-29 12:50:39,191] [INFO] Query marker FASTA was written to GCA_023819285.1_ASM2381928v1_genomic.fna/markers.fasta
[2023-06-29 12:50:39,191] [INFO] Task started: Blastn
[2023-06-29 12:50:39,192] [INFO] Running command: blastn -query GCA_023819285.1_ASM2381928v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg26b6091d-369d-4f28-93ed-6a8a8b209686/dqc_reference/reference_markers.fasta -out GCA_023819285.1_ASM2381928v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 12:50:39,677] [INFO] Task succeeded: Blastn
[2023-06-29 12:50:39,681] [INFO] Selected 5 target genomes.
[2023-06-29 12:50:39,681] [INFO] Target genome list was writen to GCA_023819285.1_ASM2381928v1_genomic.fna/target_genomes.txt
[2023-06-29 12:50:39,683] [INFO] Task started: fastANI
[2023-06-29 12:50:39,683] [INFO] Running command: fastANI --query /var/lib/cwl/stg9d233ed9-c0ba-4a0d-a03c-eae69da6186d/GCA_023819285.1_ASM2381928v1_genomic.fna.gz --refList GCA_023819285.1_ASM2381928v1_genomic.fna/target_genomes.txt --output GCA_023819285.1_ASM2381928v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 12:50:41,706] [INFO] Task succeeded: fastANI
[2023-06-29 12:50:41,707] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg26b6091d-369d-4f28-93ed-6a8a8b209686/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 12:50:41,707] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg26b6091d-369d-4f28-93ed-6a8a8b209686/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 12:50:41,709] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 12:50:41,710] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 12:50:41,710] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 12:50:41,712] [INFO] DFAST Taxonomy check result was written to GCA_023819285.1_ASM2381928v1_genomic.fna/tc_result.tsv
[2023-06-29 12:50:41,713] [INFO] ===== Taxonomy check completed =====
[2023-06-29 12:50:41,713] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 12:50:41,714] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg26b6091d-369d-4f28-93ed-6a8a8b209686/dqc_reference/checkm_data
[2023-06-29 12:50:41,718] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 12:50:41,734] [INFO] Task started: CheckM
[2023-06-29 12:50:41,735] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_023819285.1_ASM2381928v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_023819285.1_ASM2381928v1_genomic.fna/checkm_input GCA_023819285.1_ASM2381928v1_genomic.fna/checkm_result
[2023-06-29 12:50:57,001] [INFO] Task succeeded: CheckM
[2023-06-29 12:50:57,002] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 46.74%
Contamintation: 4.17%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-06-29 12:50:57,028] [INFO] ===== Completeness check finished =====
[2023-06-29 12:50:57,029] [INFO] ===== Start GTDB Search =====
[2023-06-29 12:50:57,029] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_023819285.1_ASM2381928v1_genomic.fna/markers.fasta)
[2023-06-29 12:50:57,029] [INFO] Task started: Blastn
[2023-06-29 12:50:57,029] [INFO] Running command: blastn -query GCA_023819285.1_ASM2381928v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg26b6091d-369d-4f28-93ed-6a8a8b209686/dqc_reference/reference_markers_gtdb.fasta -out GCA_023819285.1_ASM2381928v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 12:50:57,493] [INFO] Task succeeded: Blastn
[2023-06-29 12:50:57,498] [INFO] Selected 14 target genomes.
[2023-06-29 12:50:57,498] [INFO] Target genome list was writen to GCA_023819285.1_ASM2381928v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 12:50:57,513] [INFO] Task started: fastANI
[2023-06-29 12:50:57,513] [INFO] Running command: fastANI --query /var/lib/cwl/stg9d233ed9-c0ba-4a0d-a03c-eae69da6186d/GCA_023819285.1_ASM2381928v1_genomic.fna.gz --refList GCA_023819285.1_ASM2381928v1_genomic.fna/target_genomes_gtdb.txt --output GCA_023819285.1_ASM2381928v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 12:51:01,144] [INFO] Task succeeded: fastANI
[2023-06-29 12:51:01,147] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-29 12:51:01,147] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-06-29 12:51:01,149] [INFO] GTDB search result was written to GCA_023819285.1_ASM2381928v1_genomic.fna/result_gtdb.tsv
[2023-06-29 12:51:01,150] [INFO] ===== GTDB Search completed =====
[2023-06-29 12:51:01,153] [INFO] DFAST_QC result json was written to GCA_023819285.1_ASM2381928v1_genomic.fna/dqc_result.json
[2023-06-29 12:51:01,154] [INFO] DFAST_QC completed!
[2023-06-29 12:51:01,154] [INFO] Total running time: 0h0m26s