[2023-07-01 06:19:45,301] [INFO] DFAST_QC pipeline started.
[2023-07-01 06:19:45,304] [INFO] DFAST_QC version: 0.5.7
[2023-07-01 06:19:45,304] [INFO] DQC Reference Directory: /var/lib/cwl/stgbb7cf8c1-ca5a-425a-b210-2440a5c59c1e/dqc_reference
[2023-07-01 06:19:46,511] [INFO] ===== Start taxonomy check using ANI =====
[2023-07-01 06:19:46,512] [INFO] Task started: Prodigal
[2023-07-01 06:19:46,513] [INFO] Running command: gunzip -c /var/lib/cwl/stg1b584e94-0e53-42f1-ba7d-53227b963e43/GCA_024348175.1_ASM2434817v1_genomic.fna.gz | prodigal -d GCA_024348175.1_ASM2434817v1_genomic.fna/cds.fna -a GCA_024348175.1_ASM2434817v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-07-01 06:19:52,829] [INFO] Task succeeded: Prodigal
[2023-07-01 06:19:52,829] [INFO] Task started: HMMsearch
[2023-07-01 06:19:52,830] [INFO] Running command: hmmsearch --tblout GCA_024348175.1_ASM2434817v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgbb7cf8c1-ca5a-425a-b210-2440a5c59c1e/dqc_reference/reference_markers.hmm GCA_024348175.1_ASM2434817v1_genomic.fna/protein.faa > /dev/null
[2023-07-01 06:19:53,034] [INFO] Task succeeded: HMMsearch
[2023-07-01 06:19:53,036] [INFO] Found 6/6 markers.
[2023-07-01 06:19:53,084] [INFO] Query marker FASTA was written to GCA_024348175.1_ASM2434817v1_genomic.fna/markers.fasta
[2023-07-01 06:19:53,085] [INFO] Task started: Blastn
[2023-07-01 06:19:53,085] [INFO] Running command: blastn -query GCA_024348175.1_ASM2434817v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbb7cf8c1-ca5a-425a-b210-2440a5c59c1e/dqc_reference/reference_markers.fasta -out GCA_024348175.1_ASM2434817v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-07-01 06:19:53,843] [INFO] Task succeeded: Blastn
[2023-07-01 06:19:53,846] [INFO] Selected 17 target genomes.
[2023-07-01 06:19:53,847] [INFO] Target genome list was writen to GCA_024348175.1_ASM2434817v1_genomic.fna/target_genomes.txt
[2023-07-01 06:19:53,849] [INFO] Task started: fastANI
[2023-07-01 06:19:53,850] [INFO] Running command: fastANI --query /var/lib/cwl/stg1b584e94-0e53-42f1-ba7d-53227b963e43/GCA_024348175.1_ASM2434817v1_genomic.fna.gz --refList GCA_024348175.1_ASM2434817v1_genomic.fna/target_genomes.txt --output GCA_024348175.1_ASM2434817v1_genomic.fna/fastani_result.tsv --threads 1
[2023-07-01 06:20:02,316] [INFO] Task succeeded: fastANI
[2023-07-01 06:20:02,317] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgbb7cf8c1-ca5a-425a-b210-2440a5c59c1e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-07-01 06:20:02,317] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgbb7cf8c1-ca5a-425a-b210-2440a5c59c1e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-07-01 06:20:02,338] [INFO] Found 11 fastANI hits (2 hits with ANI > threshold)
[2023-07-01 06:20:02,338] [INFO] The taxonomy check result is classified as 'conclusive'.
[2023-07-01 06:20:02,338] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Bombella apis	strain=MRM1	GCA_014878255.1	1785988	1785988	type	True	99.0668	632	678	95	conclusive
Bombella apis	strain=MRM1	GCA_018221685.1	1785988	1785988	type	True	99.0539	637	678	95	conclusive
Bombella favorum	strain=TMW 2.1880	GCA_014048475.1	2039164	2039164	type	True	83.5249	536	678	95	below_threshold
Bombella intestini	strain=R-52487	GCA_002003665.1	1539051	1539051	type	True	83.2089	534	678	95	below_threshold
Gluconobacter vitians	strain=LMG 31484	GCA_015244565.1	2728102	2728102	type	True	77.662	132	678	95	below_threshold
Gluconobacter japonicus	strain=NBRC 3271	GCA_002723975.1	376620	376620	type	True	77.2747	103	678	95	below_threshold
Acidomonas methanolica	strain=NBRC 104435	GCA_007988925.1	437	437	type	True	76.9486	87	678	95	below_threshold
Oecophyllibacter saccharovorans	strain=Ha5	GCA_006542375.1	2558360	2558360	type	True	76.905	116	678	95	below_threshold
Acetobacter garciniae	strain=TBRC 12339	GCA_017377735.1	2817435	2817435	type	True	76.6641	93	678	95	below_threshold
Endobacter medicaginis	strain=CECT 8088	GCA_014192375.1	1181271	1181271	type	True	76.2707	55	678	95	below_threshold
Roseococcus microcysteis	strain=NIBR12	GCA_014764365.1	2771361	2771361	type	True	76.0149	53	678	95	below_threshold
--------------------------------------------------------------------------------
[2023-07-01 06:20:02,341] [INFO] DFAST Taxonomy check result was written to GCA_024348175.1_ASM2434817v1_genomic.fna/tc_result.tsv
[2023-07-01 06:20:02,341] [INFO] ===== Taxonomy check completed =====
[2023-07-01 06:20:02,342] [INFO] ===== Start completeness check using CheckM =====
[2023-07-01 06:20:02,342] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgbb7cf8c1-ca5a-425a-b210-2440a5c59c1e/dqc_reference/checkm_data
[2023-07-01 06:20:02,343] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-07-01 06:20:02,370] [INFO] Task started: CheckM
[2023-07-01 06:20:02,370] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_024348175.1_ASM2434817v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_024348175.1_ASM2434817v1_genomic.fna/checkm_input GCA_024348175.1_ASM2434817v1_genomic.fna/checkm_result
[2023-07-01 06:20:28,213] [INFO] Task succeeded: CheckM
[2023-07-01 06:20:28,214] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-07-01 06:20:28,234] [INFO] ===== Completeness check finished =====
[2023-07-01 06:20:28,235] [INFO] ===== Start GTDB Search =====
[2023-07-01 06:20:28,235] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_024348175.1_ASM2434817v1_genomic.fna/markers.fasta)
[2023-07-01 06:20:28,235] [INFO] Task started: Blastn
[2023-07-01 06:20:28,236] [INFO] Running command: blastn -query GCA_024348175.1_ASM2434817v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbb7cf8c1-ca5a-425a-b210-2440a5c59c1e/dqc_reference/reference_markers_gtdb.fasta -out GCA_024348175.1_ASM2434817v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-07-01 06:20:29,522] [INFO] Task succeeded: Blastn
[2023-07-01 06:20:29,527] [INFO] Selected 12 target genomes.
[2023-07-01 06:20:29,528] [INFO] Target genome list was writen to GCA_024348175.1_ASM2434817v1_genomic.fna/target_genomes_gtdb.txt
[2023-07-01 06:20:29,531] [INFO] Task started: fastANI
[2023-07-01 06:20:29,531] [INFO] Running command: fastANI --query /var/lib/cwl/stg1b584e94-0e53-42f1-ba7d-53227b963e43/GCA_024348175.1_ASM2434817v1_genomic.fna.gz --refList GCA_024348175.1_ASM2434817v1_genomic.fna/target_genomes_gtdb.txt --output GCA_024348175.1_ASM2434817v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-07-01 06:20:36,046] [INFO] Task succeeded: fastANI
[2023-07-01 06:20:36,065] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2023-07-01 06:20:36,066] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_018221685.1	s__Bombella apis	99.0539	637	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Bombella	95.0	99.16	98.65	0.96	0.94	13	conclusive
GCF_014048465.1	s__Bombella mellum	88.0017	562	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Bombella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014048475.1	s__Bombella favorum	83.5249	536	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Bombella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002003665.1	s__Bombella intestini	83.225	533	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Bombella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009725755.1	s__Bombella sp009725755	78.5494	244	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Bombella	95.0	99.22	99.22	0.99	0.99	2	-
GCF_002592045.1	s__Bombella apium_A	78.4599	220	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Bombella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000234355.1	s__Gluconobacter_C morbifer	77.8681	124	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter_C	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004346035.1	s__Acidomonas methanolica	76.9491	89	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acidomonas	95.0	99.93	99.80	0.99	0.98	4	-
GCF_017377735.1	s__Acetobacter sp017377735	76.6421	94	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014764365.1	s__Roseococcus sp014764365	76.0422	52	678	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Roseococcus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-07-01 06:20:36,081] [INFO] GTDB search result was written to GCA_024348175.1_ASM2434817v1_genomic.fna/result_gtdb.tsv
[2023-07-01 06:20:36,082] [INFO] ===== GTDB Search completed =====
[2023-07-01 06:20:36,087] [INFO] DFAST_QC result json was written to GCA_024348175.1_ASM2434817v1_genomic.fna/dqc_result.json
[2023-07-01 06:20:36,087] [INFO] DFAST_QC completed!
[2023-07-01 06:20:36,087] [INFO] Total running time: 0h0m51s