[2023-06-30 13:16:24,100] [INFO] DFAST_QC pipeline started.
[2023-06-30 13:16:24,103] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 13:16:24,103] [INFO] DQC Reference Directory: /var/lib/cwl/stgde6225b4-23df-4534-83ee-b63e13c532cb/dqc_reference
[2023-06-30 13:16:25,320] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 13:16:25,321] [INFO] Task started: Prodigal
[2023-06-30 13:16:25,321] [INFO] Running command: gunzip -c /var/lib/cwl/stga63b8943-bcf7-4b81-b0ea-3b152a5058ef/GCA_024642765.1_ASM2464276v1_genomic.fna.gz | prodigal -d GCA_024642765.1_ASM2464276v1_genomic.fna/cds.fna -a GCA_024642765.1_ASM2464276v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 13:16:35,584] [INFO] Task succeeded: Prodigal
[2023-06-30 13:16:35,585] [INFO] Task started: HMMsearch
[2023-06-30 13:16:35,585] [INFO] Running command: hmmsearch --tblout GCA_024642765.1_ASM2464276v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgde6225b4-23df-4534-83ee-b63e13c532cb/dqc_reference/reference_markers.hmm GCA_024642765.1_ASM2464276v1_genomic.fna/protein.faa > /dev/null
[2023-06-30 13:16:35,851] [INFO] Task succeeded: HMMsearch
[2023-06-30 13:16:35,852] [INFO] Found 6/6 markers.
[2023-06-30 13:16:35,889] [INFO] Query marker FASTA was written to GCA_024642765.1_ASM2464276v1_genomic.fna/markers.fasta
[2023-06-30 13:16:35,890] [INFO] Task started: Blastn
[2023-06-30 13:16:35,890] [INFO] Running command: blastn -query GCA_024642765.1_ASM2464276v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgde6225b4-23df-4534-83ee-b63e13c532cb/dqc_reference/reference_markers.fasta -out GCA_024642765.1_ASM2464276v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 13:16:36,458] [INFO] Task succeeded: Blastn
[2023-06-30 13:16:36,463] [INFO] Selected 19 target genomes.
[2023-06-30 13:16:36,464] [INFO] Target genome list was writen to GCA_024642765.1_ASM2464276v1_genomic.fna/target_genomes.txt
[2023-06-30 13:16:36,470] [INFO] Task started: fastANI
[2023-06-30 13:16:36,470] [INFO] Running command: fastANI --query /var/lib/cwl/stga63b8943-bcf7-4b81-b0ea-3b152a5058ef/GCA_024642765.1_ASM2464276v1_genomic.fna.gz --refList GCA_024642765.1_ASM2464276v1_genomic.fna/target_genomes.txt --output GCA_024642765.1_ASM2464276v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 13:16:52,967] [INFO] Task succeeded: fastANI
[2023-06-30 13:16:52,968] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgde6225b4-23df-4534-83ee-b63e13c532cb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 13:16:52,968] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgde6225b4-23df-4534-83ee-b63e13c532cb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 13:16:52,970] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 13:16:52,971] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-30 13:16:52,971] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-30 13:16:52,973] [INFO] DFAST Taxonomy check result was written to GCA_024642765.1_ASM2464276v1_genomic.fna/tc_result.tsv
[2023-06-30 13:16:52,974] [INFO] ===== Taxonomy check completed =====
[2023-06-30 13:16:52,974] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 13:16:52,975] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgde6225b4-23df-4534-83ee-b63e13c532cb/dqc_reference/checkm_data
[2023-06-30 13:16:52,979] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 13:16:53,021] [INFO] Task started: CheckM
[2023-06-30 13:16:53,022] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_024642765.1_ASM2464276v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_024642765.1_ASM2464276v1_genomic.fna/checkm_input GCA_024642765.1_ASM2464276v1_genomic.fna/checkm_result
[2023-06-30 13:17:28,386] [INFO] Task succeeded: CheckM
[2023-06-30 13:17:28,388] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.55%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-06-30 13:17:28,413] [INFO] ===== Completeness check finished =====
[2023-06-30 13:17:28,413] [INFO] ===== Start GTDB Search =====
[2023-06-30 13:17:28,414] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_024642765.1_ASM2464276v1_genomic.fna/markers.fasta)
[2023-06-30 13:17:28,414] [INFO] Task started: Blastn
[2023-06-30 13:17:28,414] [INFO] Running command: blastn -query GCA_024642765.1_ASM2464276v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgde6225b4-23df-4534-83ee-b63e13c532cb/dqc_reference/reference_markers_gtdb.fasta -out GCA_024642765.1_ASM2464276v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 13:17:29,158] [INFO] Task succeeded: Blastn
[2023-06-30 13:17:29,163] [INFO] Selected 26 target genomes.
[2023-06-30 13:17:29,164] [INFO] Target genome list was writen to GCA_024642765.1_ASM2464276v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 13:17:29,180] [INFO] Task started: fastANI
[2023-06-30 13:17:29,180] [INFO] Running command: fastANI --query /var/lib/cwl/stga63b8943-bcf7-4b81-b0ea-3b152a5058ef/GCA_024642765.1_ASM2464276v1_genomic.fna.gz --refList GCA_024642765.1_ASM2464276v1_genomic.fna/target_genomes_gtdb.txt --output GCA_024642765.1_ASM2464276v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 13:17:51,589] [INFO] Task succeeded: fastANI
[2023-06-30 13:17:51,591] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-30 13:17:51,592] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-06-30 13:17:51,594] [INFO] GTDB search result was written to GCA_024642765.1_ASM2464276v1_genomic.fna/result_gtdb.tsv
[2023-06-30 13:17:51,595] [INFO] ===== GTDB Search completed =====
[2023-06-30 13:17:51,598] [INFO] DFAST_QC result json was written to GCA_024642765.1_ASM2464276v1_genomic.fna/dqc_result.json
[2023-06-30 13:17:51,598] [INFO] DFAST_QC completed!
[2023-06-30 13:17:51,598] [INFO] Total running time: 0h1m27s