[2023-06-27 05:46:19,360] [INFO] DFAST_QC pipeline started.
[2023-06-27 05:46:19,363] [INFO] DFAST_QC version: 0.5.7
[2023-06-27 05:46:19,363] [INFO] DQC Reference Directory: /var/lib/cwl/stgfcfbd05f-191a-4c4f-9e4b-6b5fe3cab6b3/dqc_reference
[2023-06-27 05:46:20,573] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-27 05:46:20,574] [INFO] Task started: Prodigal
[2023-06-27 05:46:20,574] [INFO] Running command: gunzip -c /var/lib/cwl/stg8cfeebb3-bc37-47b1-b7aa-03500e3978b4/GCA_026396875.1_ASM2639687v1_genomic.fna.gz | prodigal -d GCA_026396875.1_ASM2639687v1_genomic.fna/cds.fna -a GCA_026396875.1_ASM2639687v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-27 05:46:23,766] [INFO] Task succeeded: Prodigal
[2023-06-27 05:46:23,767] [INFO] Task started: HMMsearch
[2023-06-27 05:46:23,767] [INFO] Running command: hmmsearch --tblout GCA_026396875.1_ASM2639687v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfcfbd05f-191a-4c4f-9e4b-6b5fe3cab6b3/dqc_reference/reference_markers.hmm GCA_026396875.1_ASM2639687v1_genomic.fna/protein.faa > /dev/null
[2023-06-27 05:46:23,990] [INFO] Task succeeded: HMMsearch
[2023-06-27 05:46:23,992] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg8cfeebb3-bc37-47b1-b7aa-03500e3978b4/GCA_026396875.1_ASM2639687v1_genomic.fna.gz]
[2023-06-27 05:46:24,006] [INFO] Query marker FASTA was written to GCA_026396875.1_ASM2639687v1_genomic.fna/markers.fasta
[2023-06-27 05:46:24,006] [INFO] Task started: Blastn
[2023-06-27 05:46:24,007] [INFO] Running command: blastn -query GCA_026396875.1_ASM2639687v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgfcfbd05f-191a-4c4f-9e4b-6b5fe3cab6b3/dqc_reference/reference_markers.fasta -out GCA_026396875.1_ASM2639687v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-27 05:46:24,563] [INFO] Task succeeded: Blastn
[2023-06-27 05:46:24,568] [INFO] Selected 9 target genomes.
[2023-06-27 05:46:24,568] [INFO] Target genome list was writen to GCA_026396875.1_ASM2639687v1_genomic.fna/target_genomes.txt
[2023-06-27 05:46:24,572] [INFO] Task started: fastANI
[2023-06-27 05:46:24,572] [INFO] Running command: fastANI --query /var/lib/cwl/stg8cfeebb3-bc37-47b1-b7aa-03500e3978b4/GCA_026396875.1_ASM2639687v1_genomic.fna.gz --refList GCA_026396875.1_ASM2639687v1_genomic.fna/target_genomes.txt --output GCA_026396875.1_ASM2639687v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-27 05:46:28,080] [INFO] Task succeeded: fastANI
[2023-06-27 05:46:28,080] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgfcfbd05f-191a-4c4f-9e4b-6b5fe3cab6b3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-27 05:46:28,081] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgfcfbd05f-191a-4c4f-9e4b-6b5fe3cab6b3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-27 05:46:28,083] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-27 05:46:28,083] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-27 05:46:28,083] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-27 05:46:28,085] [INFO] DFAST Taxonomy check result was written to GCA_026396875.1_ASM2639687v1_genomic.fna/tc_result.tsv
[2023-06-27 05:46:28,086] [INFO] ===== Taxonomy check completed =====
[2023-06-27 05:46:28,086] [INFO] ===== Start completeness check using CheckM =====
[2023-06-27 05:46:28,086] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfcfbd05f-191a-4c4f-9e4b-6b5fe3cab6b3/dqc_reference/checkm_data
[2023-06-27 05:46:28,090] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-27 05:46:28,107] [INFO] Task started: CheckM
[2023-06-27 05:46:28,108] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_026396875.1_ASM2639687v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_026396875.1_ASM2639687v1_genomic.fna/checkm_input GCA_026396875.1_ASM2639687v1_genomic.fna/checkm_result
[2023-06-27 05:46:45,288] [INFO] Task succeeded: CheckM
[2023-06-27 05:46:45,290] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-27 05:46:45,311] [INFO] ===== Completeness check finished =====
[2023-06-27 05:46:45,312] [INFO] ===== Start GTDB Search =====
[2023-06-27 05:46:45,312] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_026396875.1_ASM2639687v1_genomic.fna/markers.fasta)
[2023-06-27 05:46:45,313] [INFO] Task started: Blastn
[2023-06-27 05:46:45,313] [INFO] Running command: blastn -query GCA_026396875.1_ASM2639687v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgfcfbd05f-191a-4c4f-9e4b-6b5fe3cab6b3/dqc_reference/reference_markers_gtdb.fasta -out GCA_026396875.1_ASM2639687v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-27 05:46:46,198] [INFO] Task succeeded: Blastn
[2023-06-27 05:46:46,202] [INFO] Selected 18 target genomes.
[2023-06-27 05:46:46,202] [INFO] Target genome list was writen to GCA_026396875.1_ASM2639687v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-27 05:46:46,225] [INFO] Task started: fastANI
[2023-06-27 05:46:46,226] [INFO] Running command: fastANI --query /var/lib/cwl/stg8cfeebb3-bc37-47b1-b7aa-03500e3978b4/GCA_026396875.1_ASM2639687v1_genomic.fna.gz --refList GCA_026396875.1_ASM2639687v1_genomic.fna/target_genomes_gtdb.txt --output GCA_026396875.1_ASM2639687v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-27 05:46:51,773] [INFO] Task succeeded: fastANI
[2023-06-27 05:46:51,778] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-27 05:46:51,779] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_903847415.1	s__CAINDR01 sp903847415	79.3759	95	250	d__Bacteria;p__Patescibacteria;c__ABY1;o__BM507;f__UBA12465;g__CAINDR01	95.0	99.87	99.87	0.91	0.91	2	-
GCA_903839955.1	s__CAINDR01 sp903839955	78.6902	67	250	d__Bacteria;p__Patescibacteria;c__ABY1;o__BM507;f__UBA12465;g__CAINDR01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-27 05:46:51,793] [INFO] GTDB search result was written to GCA_026396875.1_ASM2639687v1_genomic.fna/result_gtdb.tsv
[2023-06-27 05:46:51,793] [INFO] ===== GTDB Search completed =====
[2023-06-27 05:46:51,797] [INFO] DFAST_QC result json was written to GCA_026396875.1_ASM2639687v1_genomic.fna/dqc_result.json
[2023-06-27 05:46:51,797] [INFO] DFAST_QC completed!
[2023-06-27 05:46:51,797] [INFO] Total running time: 0h0m32s