[2023-06-27 23:06:19,560] [INFO] DFAST_QC pipeline started.
[2023-06-27 23:06:19,565] [INFO] DFAST_QC version: 0.5.7
[2023-06-27 23:06:19,566] [INFO] DQC Reference Directory: /var/lib/cwl/stg3e0b097e-8d42-495d-a356-05866084263a/dqc_reference
[2023-06-27 23:06:21,872] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-27 23:06:21,873] [INFO] Task started: Prodigal
[2023-06-27 23:06:21,874] [INFO] Running command: gunzip -c /var/lib/cwl/stg7935ce69-1ee7-4cec-88cb-6ae0b99d1a80/GCA_026415175.1_ASM2641517v1_genomic.fna.gz | prodigal -d GCA_026415175.1_ASM2641517v1_genomic.fna/cds.fna -a GCA_026415175.1_ASM2641517v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-27 23:06:34,315] [INFO] Task succeeded: Prodigal
[2023-06-27 23:06:34,316] [INFO] Task started: HMMsearch
[2023-06-27 23:06:34,316] [INFO] Running command: hmmsearch --tblout GCA_026415175.1_ASM2641517v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3e0b097e-8d42-495d-a356-05866084263a/dqc_reference/reference_markers.hmm GCA_026415175.1_ASM2641517v1_genomic.fna/protein.faa > /dev/null
[2023-06-27 23:06:34,565] [INFO] Task succeeded: HMMsearch
[2023-06-27 23:06:34,568] [INFO] Found 6/6 markers.
[2023-06-27 23:06:34,596] [INFO] Query marker FASTA was written to GCA_026415175.1_ASM2641517v1_genomic.fna/markers.fasta
[2023-06-27 23:06:34,596] [INFO] Task started: Blastn
[2023-06-27 23:06:34,597] [INFO] Running command: blastn -query GCA_026415175.1_ASM2641517v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3e0b097e-8d42-495d-a356-05866084263a/dqc_reference/reference_markers.fasta -out GCA_026415175.1_ASM2641517v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-27 23:06:35,193] [INFO] Task succeeded: Blastn
[2023-06-27 23:06:35,200] [INFO] Selected 17 target genomes.
[2023-06-27 23:06:35,201] [INFO] Target genome list was writen to GCA_026415175.1_ASM2641517v1_genomic.fna/target_genomes.txt
[2023-06-27 23:06:35,204] [INFO] Task started: fastANI
[2023-06-27 23:06:35,205] [INFO] Running command: fastANI --query /var/lib/cwl/stg7935ce69-1ee7-4cec-88cb-6ae0b99d1a80/GCA_026415175.1_ASM2641517v1_genomic.fna.gz --refList GCA_026415175.1_ASM2641517v1_genomic.fna/target_genomes.txt --output GCA_026415175.1_ASM2641517v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-27 23:06:46,638] [INFO] Task succeeded: fastANI
[2023-06-27 23:06:46,639] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3e0b097e-8d42-495d-a356-05866084263a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-27 23:06:46,639] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3e0b097e-8d42-495d-a356-05866084263a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-27 23:06:46,641] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-27 23:06:46,642] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-27 23:06:46,642] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-27 23:06:46,647] [INFO] DFAST Taxonomy check result was written to GCA_026415175.1_ASM2641517v1_genomic.fna/tc_result.tsv
[2023-06-27 23:06:46,648] [INFO] ===== Taxonomy check completed =====
[2023-06-27 23:06:46,648] [INFO] ===== Start completeness check using CheckM =====
[2023-06-27 23:06:46,649] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3e0b097e-8d42-495d-a356-05866084263a/dqc_reference/checkm_data
[2023-06-27 23:06:46,652] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-27 23:06:46,689] [INFO] Task started: CheckM
[2023-06-27 23:06:46,689] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_026415175.1_ASM2641517v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_026415175.1_ASM2641517v1_genomic.fna/checkm_input GCA_026415175.1_ASM2641517v1_genomic.fna/checkm_result
[2023-06-27 23:07:25,109] [INFO] Task succeeded: CheckM
[2023-06-27 23:07:25,110] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.46%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-27 23:07:25,135] [INFO] ===== Completeness check finished =====
[2023-06-27 23:07:25,135] [INFO] ===== Start GTDB Search =====
[2023-06-27 23:07:25,136] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_026415175.1_ASM2641517v1_genomic.fna/markers.fasta)
[2023-06-27 23:07:25,136] [INFO] Task started: Blastn
[2023-06-27 23:07:25,136] [INFO] Running command: blastn -query GCA_026415175.1_ASM2641517v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3e0b097e-8d42-495d-a356-05866084263a/dqc_reference/reference_markers_gtdb.fasta -out GCA_026415175.1_ASM2641517v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-27 23:07:26,041] [INFO] Task succeeded: Blastn
[2023-06-27 23:07:26,048] [INFO] Selected 24 target genomes.
[2023-06-27 23:07:26,048] [INFO] Target genome list was writen to GCA_026415175.1_ASM2641517v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-27 23:07:26,078] [INFO] Task started: fastANI
[2023-06-27 23:07:26,078] [INFO] Running command: fastANI --query /var/lib/cwl/stg7935ce69-1ee7-4cec-88cb-6ae0b99d1a80/GCA_026415175.1_ASM2641517v1_genomic.fna.gz --refList GCA_026415175.1_ASM2641517v1_genomic.fna/target_genomes_gtdb.txt --output GCA_026415175.1_ASM2641517v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-27 23:07:42,126] [INFO] Task succeeded: fastANI
[2023-06-27 23:07:42,130] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-27 23:07:42,130] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_015295805.1	s__DVEF01 sp015295805	99.8749	861	920	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Cyanobacteriales;f__Cyanobacteriaceae;g__DVEF01	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2023-06-27 23:07:42,132] [INFO] GTDB search result was written to GCA_026415175.1_ASM2641517v1_genomic.fna/result_gtdb.tsv
[2023-06-27 23:07:42,133] [INFO] ===== GTDB Search completed =====
[2023-06-27 23:07:42,135] [INFO] DFAST_QC result json was written to GCA_026415175.1_ASM2641517v1_genomic.fna/dqc_result.json
[2023-06-27 23:07:42,136] [INFO] DFAST_QC completed!
[2023-06-27 23:07:42,136] [INFO] Total running time: 0h1m23s