[2023-06-27 21:45:18,830] [INFO] DFAST_QC pipeline started.
[2023-06-27 21:45:18,835] [INFO] DFAST_QC version: 0.5.7
[2023-06-27 21:45:18,835] [INFO] DQC Reference Directory: /var/lib/cwl/stg42f7be49-3e1c-456e-8cd3-010137ca0305/dqc_reference
[2023-06-27 21:45:20,053] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-27 21:45:20,054] [INFO] Task started: Prodigal
[2023-06-27 21:45:20,054] [INFO] Running command: gunzip -c /var/lib/cwl/stga4551cb7-ff31-4d29-b509-78f03139000e/GCA_026998115.1_ASM2699811v1_genomic.fna.gz | prodigal -d GCA_026998115.1_ASM2699811v1_genomic.fna/cds.fna -a GCA_026998115.1_ASM2699811v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-27 21:45:24,750] [INFO] Task succeeded: Prodigal
[2023-06-27 21:45:24,750] [INFO] Task started: HMMsearch
[2023-06-27 21:45:24,750] [INFO] Running command: hmmsearch --tblout GCA_026998115.1_ASM2699811v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg42f7be49-3e1c-456e-8cd3-010137ca0305/dqc_reference/reference_markers.hmm GCA_026998115.1_ASM2699811v1_genomic.fna/protein.faa > /dev/null
[2023-06-27 21:45:24,920] [INFO] Task succeeded: HMMsearch
[2023-06-27 21:45:24,921] [WARNING] Found 4/6 markers. [/var/lib/cwl/stga4551cb7-ff31-4d29-b509-78f03139000e/GCA_026998115.1_ASM2699811v1_genomic.fna.gz]
[2023-06-27 21:45:25,063] [INFO] Query marker FASTA was written to GCA_026998115.1_ASM2699811v1_genomic.fna/markers.fasta
[2023-06-27 21:45:25,063] [INFO] Task started: Blastn
[2023-06-27 21:45:25,064] [INFO] Running command: blastn -query GCA_026998115.1_ASM2699811v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg42f7be49-3e1c-456e-8cd3-010137ca0305/dqc_reference/reference_markers.fasta -out GCA_026998115.1_ASM2699811v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-27 21:45:25,600] [INFO] Task succeeded: Blastn
[2023-06-27 21:45:25,604] [INFO] Selected 6 target genomes.
[2023-06-27 21:45:25,605] [INFO] Target genome list was writen to GCA_026998115.1_ASM2699811v1_genomic.fna/target_genomes.txt
[2023-06-27 21:45:25,607] [INFO] Task started: fastANI
[2023-06-27 21:45:25,607] [INFO] Running command: fastANI --query /var/lib/cwl/stga4551cb7-ff31-4d29-b509-78f03139000e/GCA_026998115.1_ASM2699811v1_genomic.fna.gz --refList GCA_026998115.1_ASM2699811v1_genomic.fna/target_genomes.txt --output GCA_026998115.1_ASM2699811v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-27 21:45:29,743] [INFO] Task succeeded: fastANI
[2023-06-27 21:45:29,743] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg42f7be49-3e1c-456e-8cd3-010137ca0305/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-27 21:45:29,744] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg42f7be49-3e1c-456e-8cd3-010137ca0305/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-27 21:45:29,746] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-27 21:45:29,746] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-27 21:45:29,746] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-27 21:45:29,749] [INFO] DFAST Taxonomy check result was written to GCA_026998115.1_ASM2699811v1_genomic.fna/tc_result.tsv
[2023-06-27 21:45:29,749] [INFO] ===== Taxonomy check completed =====
[2023-06-27 21:45:29,750] [INFO] ===== Start completeness check using CheckM =====
[2023-06-27 21:45:29,750] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg42f7be49-3e1c-456e-8cd3-010137ca0305/dqc_reference/checkm_data
[2023-06-27 21:45:29,754] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-27 21:45:29,775] [INFO] Task started: CheckM
[2023-06-27 21:45:29,775] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_026998115.1_ASM2699811v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_026998115.1_ASM2699811v1_genomic.fna/checkm_input GCA_026998115.1_ASM2699811v1_genomic.fna/checkm_result
[2023-06-27 21:45:50,130] [INFO] Task succeeded: CheckM
[2023-06-27 21:45:50,131] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 72.94%
Contamintation: 2.06%
Strain heterogeneity: 83.33%
--------------------------------------------------------------------------------
[2023-06-27 21:45:50,156] [INFO] ===== Completeness check finished =====
[2023-06-27 21:45:50,156] [INFO] ===== Start GTDB Search =====
[2023-06-27 21:45:50,157] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_026998115.1_ASM2699811v1_genomic.fna/markers.fasta)
[2023-06-27 21:45:50,157] [INFO] Task started: Blastn
[2023-06-27 21:45:50,157] [INFO] Running command: blastn -query GCA_026998115.1_ASM2699811v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg42f7be49-3e1c-456e-8cd3-010137ca0305/dqc_reference/reference_markers_gtdb.fasta -out GCA_026998115.1_ASM2699811v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-27 21:45:50,850] [INFO] Task succeeded: Blastn
[2023-06-27 21:45:50,855] [INFO] Selected 9 target genomes.
[2023-06-27 21:45:50,855] [INFO] Target genome list was writen to GCA_026998115.1_ASM2699811v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-27 21:45:50,861] [INFO] Task started: fastANI
[2023-06-27 21:45:50,861] [INFO] Running command: fastANI --query /var/lib/cwl/stga4551cb7-ff31-4d29-b509-78f03139000e/GCA_026998115.1_ASM2699811v1_genomic.fna.gz --refList GCA_026998115.1_ASM2699811v1_genomic.fna/target_genomes_gtdb.txt --output GCA_026998115.1_ASM2699811v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-27 21:45:53,110] [INFO] Task succeeded: fastANI
[2023-06-27 21:45:53,116] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-27 21:45:53,116] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_015487835.1	s__Hydrogenivirga sp015487835	96.6773	72	82	d__Bacteria;p__Aquificota;c__Aquificae;o__Aquificales;f__Aquificaceae;g__Hydrogenivirga	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_015490115.1	s__Hydrogenivirga sp015490115	80.6579	59	82	d__Bacteria;p__Aquificota;c__Aquificae;o__Aquificales;f__Aquificaceae;g__Hydrogenivirga	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-27 21:45:53,119] [INFO] GTDB search result was written to GCA_026998115.1_ASM2699811v1_genomic.fna/result_gtdb.tsv
[2023-06-27 21:45:53,119] [INFO] ===== GTDB Search completed =====
[2023-06-27 21:45:53,121] [INFO] DFAST_QC result json was written to GCA_026998115.1_ASM2699811v1_genomic.fna/dqc_result.json
[2023-06-27 21:45:53,122] [INFO] DFAST_QC completed!
[2023-06-27 21:45:53,122] [INFO] Total running time: 0h0m34s