[2023-06-27 22:54:53,300] [INFO] DFAST_QC pipeline started.
[2023-06-27 22:54:53,302] [INFO] DFAST_QC version: 0.5.7
[2023-06-27 22:54:53,302] [INFO] DQC Reference Directory: /var/lib/cwl/stgde30dabc-c48f-4ac8-9441-797c2b25e64f/dqc_reference
[2023-06-27 22:54:54,621] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-27 22:54:54,622] [INFO] Task started: Prodigal
[2023-06-27 22:54:54,623] [INFO] Running command: gunzip -c /var/lib/cwl/stgd288a9e4-49fa-4902-8de2-3fb5495e1ba7/GCA_027004645.1_ASM2700464v1_genomic.fna.gz | prodigal -d GCA_027004645.1_ASM2700464v1_genomic.fna/cds.fna -a GCA_027004645.1_ASM2700464v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-27 22:54:56,683] [INFO] Task succeeded: Prodigal
[2023-06-27 22:54:56,683] [INFO] Task started: HMMsearch
[2023-06-27 22:54:56,683] [INFO] Running command: hmmsearch --tblout GCA_027004645.1_ASM2700464v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgde30dabc-c48f-4ac8-9441-797c2b25e64f/dqc_reference/reference_markers.hmm GCA_027004645.1_ASM2700464v1_genomic.fna/protein.faa > /dev/null
[2023-06-27 22:54:56,877] [INFO] Task succeeded: HMMsearch
[2023-06-27 22:54:56,878] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgd288a9e4-49fa-4902-8de2-3fb5495e1ba7/GCA_027004645.1_ASM2700464v1_genomic.fna.gz]
[2023-06-27 22:54:56,891] [INFO] Query marker FASTA was written to GCA_027004645.1_ASM2700464v1_genomic.fna/markers.fasta
[2023-06-27 22:54:56,892] [INFO] Task started: Blastn
[2023-06-27 22:54:56,892] [INFO] Running command: blastn -query GCA_027004645.1_ASM2700464v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgde30dabc-c48f-4ac8-9441-797c2b25e64f/dqc_reference/reference_markers.fasta -out GCA_027004645.1_ASM2700464v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-27 22:54:57,460] [INFO] Task succeeded: Blastn
[2023-06-27 22:54:57,465] [INFO] Selected 14 target genomes.
[2023-06-27 22:54:57,465] [INFO] Target genome list was writen to GCA_027004645.1_ASM2700464v1_genomic.fna/target_genomes.txt
[2023-06-27 22:54:57,474] [INFO] Task started: fastANI
[2023-06-27 22:54:57,474] [INFO] Running command: fastANI --query /var/lib/cwl/stgd288a9e4-49fa-4902-8de2-3fb5495e1ba7/GCA_027004645.1_ASM2700464v1_genomic.fna.gz --refList GCA_027004645.1_ASM2700464v1_genomic.fna/target_genomes.txt --output GCA_027004645.1_ASM2700464v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-27 22:55:04,301] [INFO] Task succeeded: fastANI
[2023-06-27 22:55:04,302] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgde30dabc-c48f-4ac8-9441-797c2b25e64f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-27 22:55:04,302] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgde30dabc-c48f-4ac8-9441-797c2b25e64f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-27 22:55:04,303] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-27 22:55:04,304] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-27 22:55:04,304] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-27 22:55:04,306] [INFO] DFAST Taxonomy check result was written to GCA_027004645.1_ASM2700464v1_genomic.fna/tc_result.tsv
[2023-06-27 22:55:04,306] [INFO] ===== Taxonomy check completed =====
[2023-06-27 22:55:04,306] [INFO] ===== Start completeness check using CheckM =====
[2023-06-27 22:55:04,307] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgde30dabc-c48f-4ac8-9441-797c2b25e64f/dqc_reference/checkm_data
[2023-06-27 22:55:04,309] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-27 22:55:04,323] [INFO] Task started: CheckM
[2023-06-27 22:55:04,323] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_027004645.1_ASM2700464v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_027004645.1_ASM2700464v1_genomic.fna/checkm_input GCA_027004645.1_ASM2700464v1_genomic.fna/checkm_result
[2023-06-27 22:55:18,870] [INFO] Task succeeded: CheckM
[2023-06-27 22:55:18,871] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 60.13%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-27 22:55:18,901] [INFO] ===== Completeness check finished =====
[2023-06-27 22:55:18,902] [INFO] ===== Start GTDB Search =====
[2023-06-27 22:55:18,902] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_027004645.1_ASM2700464v1_genomic.fna/markers.fasta)
[2023-06-27 22:55:18,902] [INFO] Task started: Blastn
[2023-06-27 22:55:18,902] [INFO] Running command: blastn -query GCA_027004645.1_ASM2700464v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgde30dabc-c48f-4ac8-9441-797c2b25e64f/dqc_reference/reference_markers_gtdb.fasta -out GCA_027004645.1_ASM2700464v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-27 22:55:19,670] [INFO] Task succeeded: Blastn
[2023-06-27 22:55:19,674] [INFO] Selected 14 target genomes.
[2023-06-27 22:55:19,674] [INFO] Target genome list was writen to GCA_027004645.1_ASM2700464v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-27 22:55:19,686] [INFO] Task started: fastANI
[2023-06-27 22:55:19,686] [INFO] Running command: fastANI --query /var/lib/cwl/stgd288a9e4-49fa-4902-8de2-3fb5495e1ba7/GCA_027004645.1_ASM2700464v1_genomic.fna.gz --refList GCA_027004645.1_ASM2700464v1_genomic.fna/target_genomes_gtdb.txt --output GCA_027004645.1_ASM2700464v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-27 22:55:25,333] [INFO] Task succeeded: fastANI
[2023-06-27 22:55:25,336] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-27 22:55:25,336] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_015486555.1	s__S014-63 sp015486555	99.0426	138	187	d__Bacteria;p__Patescibacteria;c__Microgenomatia;o__JAADGY01;f__JAADGY01;g__S014-63	95.0	98.91	98.91	0.79	0.79	2	conclusive
--------------------------------------------------------------------------------
[2023-06-27 22:55:25,339] [INFO] GTDB search result was written to GCA_027004645.1_ASM2700464v1_genomic.fna/result_gtdb.tsv
[2023-06-27 22:55:25,339] [INFO] ===== GTDB Search completed =====
[2023-06-27 22:55:25,342] [INFO] DFAST_QC result json was written to GCA_027004645.1_ASM2700464v1_genomic.fna/dqc_result.json
[2023-06-27 22:55:25,342] [INFO] DFAST_QC completed!
[2023-06-27 22:55:25,342] [INFO] Total running time: 0h0m32s