[2023-06-29 11:44:45,780] [INFO] DFAST_QC pipeline started.
[2023-06-29 11:44:45,852] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 11:44:45,852] [INFO] DQC Reference Directory: /var/lib/cwl/stg470d6dd0-2afe-40eb-9b95-ca94b274c841/dqc_reference
[2023-06-29 11:44:46,940] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 11:44:46,940] [INFO] Task started: Prodigal
[2023-06-29 11:44:46,940] [INFO] Running command: gunzip -c /var/lib/cwl/stgaf72bd88-b801-433e-aefc-de18fe412904/GCA_027008765.1_ASM2700876v1_genomic.fna.gz | prodigal -d GCA_027008765.1_ASM2700876v1_genomic.fna/cds.fna -a GCA_027008765.1_ASM2700876v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 11:44:53,069] [INFO] Task succeeded: Prodigal
[2023-06-29 11:44:53,069] [INFO] Task started: HMMsearch
[2023-06-29 11:44:53,069] [INFO] Running command: hmmsearch --tblout GCA_027008765.1_ASM2700876v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg470d6dd0-2afe-40eb-9b95-ca94b274c841/dqc_reference/reference_markers.hmm GCA_027008765.1_ASM2700876v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 11:44:53,249] [INFO] Task succeeded: HMMsearch
[2023-06-29 11:44:53,250] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgaf72bd88-b801-433e-aefc-de18fe412904/GCA_027008765.1_ASM2700876v1_genomic.fna.gz]
[2023-06-29 11:44:53,311] [INFO] Query marker FASTA was written to GCA_027008765.1_ASM2700876v1_genomic.fna/markers.fasta
[2023-06-29 11:44:53,311] [INFO] Task started: Blastn
[2023-06-29 11:44:53,312] [INFO] Running command: blastn -query GCA_027008765.1_ASM2700876v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg470d6dd0-2afe-40eb-9b95-ca94b274c841/dqc_reference/reference_markers.fasta -out GCA_027008765.1_ASM2700876v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 11:44:53,789] [INFO] Task succeeded: Blastn
[2023-06-29 11:44:53,886] [INFO] Selected 7 target genomes.
[2023-06-29 11:44:53,886] [INFO] Target genome list was writen to GCA_027008765.1_ASM2700876v1_genomic.fna/target_genomes.txt
[2023-06-29 11:44:53,887] [INFO] Task started: fastANI
[2023-06-29 11:44:53,887] [INFO] Running command: fastANI --query /var/lib/cwl/stgaf72bd88-b801-433e-aefc-de18fe412904/GCA_027008765.1_ASM2700876v1_genomic.fna.gz --refList GCA_027008765.1_ASM2700876v1_genomic.fna/target_genomes.txt --output GCA_027008765.1_ASM2700876v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 11:44:56,430] [INFO] Task succeeded: fastANI
[2023-06-29 11:44:56,430] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg470d6dd0-2afe-40eb-9b95-ca94b274c841/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 11:44:56,430] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg470d6dd0-2afe-40eb-9b95-ca94b274c841/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 11:44:56,432] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 11:44:56,432] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 11:44:56,432] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 11:44:56,452] [INFO] DFAST Taxonomy check result was written to GCA_027008765.1_ASM2700876v1_genomic.fna/tc_result.tsv
[2023-06-29 11:44:56,453] [INFO] ===== Taxonomy check completed =====
[2023-06-29 11:44:56,453] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 11:44:56,453] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg470d6dd0-2afe-40eb-9b95-ca94b274c841/dqc_reference/checkm_data
[2023-06-29 11:44:56,455] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 11:44:56,474] [INFO] Task started: CheckM
[2023-06-29 11:44:56,474] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_027008765.1_ASM2700876v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_027008765.1_ASM2700876v1_genomic.fna/checkm_input GCA_027008765.1_ASM2700876v1_genomic.fna/checkm_result
[2023-06-29 11:45:19,651] [INFO] Task succeeded: CheckM
[2023-06-29 11:45:19,652] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 75.00%
Contamintation: 0.90%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-06-29 11:45:19,668] [INFO] ===== Completeness check finished =====
[2023-06-29 11:45:19,668] [INFO] ===== Start GTDB Search =====
[2023-06-29 11:45:19,669] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_027008765.1_ASM2700876v1_genomic.fna/markers.fasta)
[2023-06-29 11:45:19,669] [INFO] Task started: Blastn
[2023-06-29 11:45:19,669] [INFO] Running command: blastn -query GCA_027008765.1_ASM2700876v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg470d6dd0-2afe-40eb-9b95-ca94b274c841/dqc_reference/reference_markers_gtdb.fasta -out GCA_027008765.1_ASM2700876v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 11:45:20,294] [INFO] Task succeeded: Blastn
[2023-06-29 11:45:20,298] [INFO] Selected 18 target genomes.
[2023-06-29 11:45:20,298] [INFO] Target genome list was writen to GCA_027008765.1_ASM2700876v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 11:45:20,304] [INFO] Task started: fastANI
[2023-06-29 11:45:20,305] [INFO] Running command: fastANI --query /var/lib/cwl/stgaf72bd88-b801-433e-aefc-de18fe412904/GCA_027008765.1_ASM2700876v1_genomic.fna.gz --refList GCA_027008765.1_ASM2700876v1_genomic.fna/target_genomes_gtdb.txt --output GCA_027008765.1_ASM2700876v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 11:45:27,510] [INFO] Task succeeded: fastANI
[2023-06-29 11:45:27,513] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-29 11:45:27,513] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_015494735.1	s__S143-66 sp015494735	80.7772	141	321	d__Bacteria;p__WOR-3;c__Hydrothermia;o__LBFQ01;f__DSFT01;g__S143-66	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-29 11:45:27,515] [INFO] GTDB search result was written to GCA_027008765.1_ASM2700876v1_genomic.fna/result_gtdb.tsv
[2023-06-29 11:45:27,515] [INFO] ===== GTDB Search completed =====
[2023-06-29 11:45:27,517] [INFO] DFAST_QC result json was written to GCA_027008765.1_ASM2700876v1_genomic.fna/dqc_result.json
[2023-06-29 11:45:27,517] [INFO] DFAST_QC completed!
[2023-06-29 11:45:27,517] [INFO] Total running time: 0h0m42s