[2023-06-29 13:20:35,120] [INFO] DFAST_QC pipeline started.
[2023-06-29 13:20:35,122] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 13:20:35,123] [INFO] DQC Reference Directory: /var/lib/cwl/stge87c14bb-b8be-4653-921a-ed78d7a15127/dqc_reference
[2023-06-29 13:20:36,415] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 13:20:36,416] [INFO] Task started: Prodigal
[2023-06-29 13:20:36,416] [INFO] Running command: gunzip -c /var/lib/cwl/stgb6eca3b1-ccef-4cc7-89c5-78962704ecbe/GCA_027310025.1_ASM2731002v1_genomic.fna.gz | prodigal -d GCA_027310025.1_ASM2731002v1_genomic.fna/cds.fna -a GCA_027310025.1_ASM2731002v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 13:20:40,913] [INFO] Task succeeded: Prodigal
[2023-06-29 13:20:40,913] [INFO] Task started: HMMsearch
[2023-06-29 13:20:40,914] [INFO] Running command: hmmsearch --tblout GCA_027310025.1_ASM2731002v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge87c14bb-b8be-4653-921a-ed78d7a15127/dqc_reference/reference_markers.hmm GCA_027310025.1_ASM2731002v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 13:20:41,122] [INFO] Task succeeded: HMMsearch
[2023-06-29 13:20:41,124] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgb6eca3b1-ccef-4cc7-89c5-78962704ecbe/GCA_027310025.1_ASM2731002v1_genomic.fna.gz]
[2023-06-29 13:20:41,141] [INFO] Query marker FASTA was written to GCA_027310025.1_ASM2731002v1_genomic.fna/markers.fasta
[2023-06-29 13:20:41,141] [INFO] Task started: Blastn
[2023-06-29 13:20:41,141] [INFO] Running command: blastn -query GCA_027310025.1_ASM2731002v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge87c14bb-b8be-4653-921a-ed78d7a15127/dqc_reference/reference_markers.fasta -out GCA_027310025.1_ASM2731002v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 13:20:41,673] [INFO] Task succeeded: Blastn
[2023-06-29 13:20:41,677] [INFO] Selected 0 target genomes.
[2023-06-29 13:20:41,677] [INFO] Target genome list was writen to GCA_027310025.1_ASM2731002v1_genomic.fna/target_genomes.txt
[2023-06-29 13:20:41,678] [ERROR] File is empty. [GCA_027310025.1_ASM2731002v1_genomic.fna/target_genomes.txt]
[2023-06-29 13:20:41,678] [ERROR] Task failed. No target genome found.
[2023-06-29 13:20:41,678] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 13:20:41,678] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge87c14bb-b8be-4653-921a-ed78d7a15127/dqc_reference/checkm_data
[2023-06-29 13:20:41,681] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 13:20:41,698] [INFO] Task started: CheckM
[2023-06-29 13:20:41,698] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_027310025.1_ASM2731002v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_027310025.1_ASM2731002v1_genomic.fna/checkm_input GCA_027310025.1_ASM2731002v1_genomic.fna/checkm_result
[2023-06-29 13:21:01,671] [INFO] Task succeeded: CheckM
[2023-06-29 13:21:01,673] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.95%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 13:21:01,697] [INFO] ===== Completeness check finished =====
[2023-06-29 13:21:01,698] [INFO] ===== Start GTDB Search =====
[2023-06-29 13:21:01,698] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_027310025.1_ASM2731002v1_genomic.fna/markers.fasta)
[2023-06-29 13:21:01,699] [INFO] Task started: Blastn
[2023-06-29 13:21:01,699] [INFO] Running command: blastn -query GCA_027310025.1_ASM2731002v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge87c14bb-b8be-4653-921a-ed78d7a15127/dqc_reference/reference_markers_gtdb.fasta -out GCA_027310025.1_ASM2731002v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 13:21:02,417] [INFO] Task succeeded: Blastn
[2023-06-29 13:21:02,428] [INFO] Selected 6 target genomes.
[2023-06-29 13:21:02,429] [INFO] Target genome list was writen to GCA_027310025.1_ASM2731002v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 13:21:02,433] [INFO] Task started: fastANI
[2023-06-29 13:21:02,433] [INFO] Running command: fastANI --query /var/lib/cwl/stgb6eca3b1-ccef-4cc7-89c5-78962704ecbe/GCA_027310025.1_ASM2731002v1_genomic.fna.gz --refList GCA_027310025.1_ASM2731002v1_genomic.fna/target_genomes_gtdb.txt --output GCA_027310025.1_ASM2731002v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 13:21:05,456] [INFO] Task succeeded: fastANI
[2023-06-29 13:21:05,458] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-29 13:21:05,458] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-06-29 13:21:05,461] [INFO] GTDB search result was written to GCA_027310025.1_ASM2731002v1_genomic.fna/result_gtdb.tsv
[2023-06-29 13:21:05,462] [INFO] ===== GTDB Search completed =====
[2023-06-29 13:21:05,465] [INFO] DFAST_QC result json was written to GCA_027310025.1_ASM2731002v1_genomic.fna/dqc_result.json
[2023-06-29 13:21:05,465] [INFO] DFAST_QC completed!
[2023-06-29 13:21:05,465] [INFO] Total running time: 0h0m30s