[2023-06-29 01:46:08,743] [INFO] DFAST_QC pipeline started.
[2023-06-29 01:46:08,745] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 01:46:08,745] [INFO] DQC Reference Directory: /var/lib/cwl/stg3a2269af-c75b-42b7-8b96-f6ed7f64c58c/dqc_reference
[2023-06-29 01:46:10,348] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 01:46:10,349] [INFO] Task started: Prodigal
[2023-06-29 01:46:10,349] [INFO] Running command: gunzip -c /var/lib/cwl/stgc4b31a83-6071-47c0-9c26-71ec3c8b5fca/GCA_027310065.1_ASM2731006v1_genomic.fna.gz | prodigal -d GCA_027310065.1_ASM2731006v1_genomic.fna/cds.fna -a GCA_027310065.1_ASM2731006v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 01:46:14,002] [INFO] Task succeeded: Prodigal
[2023-06-29 01:46:14,003] [INFO] Task started: HMMsearch
[2023-06-29 01:46:14,003] [INFO] Running command: hmmsearch --tblout GCA_027310065.1_ASM2731006v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3a2269af-c75b-42b7-8b96-f6ed7f64c58c/dqc_reference/reference_markers.hmm GCA_027310065.1_ASM2731006v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 01:46:14,188] [INFO] Task succeeded: HMMsearch
[2023-06-29 01:46:14,189] [WARNING] Found 2/6 markers. [/var/lib/cwl/stgc4b31a83-6071-47c0-9c26-71ec3c8b5fca/GCA_027310065.1_ASM2731006v1_genomic.fna.gz]
[2023-06-29 01:46:14,200] [INFO] Query marker FASTA was written to GCA_027310065.1_ASM2731006v1_genomic.fna/markers.fasta
[2023-06-29 01:46:14,201] [INFO] Task started: Blastn
[2023-06-29 01:46:14,201] [INFO] Running command: blastn -query GCA_027310065.1_ASM2731006v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3a2269af-c75b-42b7-8b96-f6ed7f64c58c/dqc_reference/reference_markers.fasta -out GCA_027310065.1_ASM2731006v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 01:46:14,738] [INFO] Task succeeded: Blastn
[2023-06-29 01:46:14,741] [INFO] Selected 0 target genomes.
[2023-06-29 01:46:14,742] [INFO] Target genome list was writen to GCA_027310065.1_ASM2731006v1_genomic.fna/target_genomes.txt
[2023-06-29 01:46:14,742] [ERROR] File is empty. [GCA_027310065.1_ASM2731006v1_genomic.fna/target_genomes.txt]
[2023-06-29 01:46:14,742] [ERROR] Task failed. No target genome found.
[2023-06-29 01:46:14,742] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 01:46:14,742] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3a2269af-c75b-42b7-8b96-f6ed7f64c58c/dqc_reference/checkm_data
[2023-06-29 01:46:14,745] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 01:46:14,755] [INFO] Task started: CheckM
[2023-06-29 01:46:14,755] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_027310065.1_ASM2731006v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_027310065.1_ASM2731006v1_genomic.fna/checkm_input GCA_027310065.1_ASM2731006v1_genomic.fna/checkm_result
[2023-06-29 01:46:33,166] [INFO] Task succeeded: CheckM
[2023-06-29 01:46:33,167] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 73.20%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 01:46:33,184] [INFO] ===== Completeness check finished =====
[2023-06-29 01:46:33,184] [INFO] ===== Start GTDB Search =====
[2023-06-29 01:46:33,185] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_027310065.1_ASM2731006v1_genomic.fna/markers.fasta)
[2023-06-29 01:46:33,185] [INFO] Task started: Blastn
[2023-06-29 01:46:33,186] [INFO] Running command: blastn -query GCA_027310065.1_ASM2731006v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3a2269af-c75b-42b7-8b96-f6ed7f64c58c/dqc_reference/reference_markers_gtdb.fasta -out GCA_027310065.1_ASM2731006v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 01:46:33,778] [INFO] Task succeeded: Blastn
[2023-06-29 01:46:33,781] [INFO] Selected 1 target genomes.
[2023-06-29 01:46:33,782] [INFO] Target genome list was writen to GCA_027310065.1_ASM2731006v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 01:46:33,783] [INFO] Task started: fastANI
[2023-06-29 01:46:33,783] [INFO] Running command: fastANI --query /var/lib/cwl/stgc4b31a83-6071-47c0-9c26-71ec3c8b5fca/GCA_027310065.1_ASM2731006v1_genomic.fna.gz --refList GCA_027310065.1_ASM2731006v1_genomic.fna/target_genomes_gtdb.txt --output GCA_027310065.1_ASM2731006v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 01:46:34,128] [INFO] Task succeeded: fastANI
[2023-06-29 01:46:34,130] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-29 01:46:34,131] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-06-29 01:46:34,132] [INFO] GTDB search result was written to GCA_027310065.1_ASM2731006v1_genomic.fna/result_gtdb.tsv
[2023-06-29 01:46:34,133] [INFO] ===== GTDB Search completed =====
[2023-06-29 01:46:34,136] [INFO] DFAST_QC result json was written to GCA_027310065.1_ASM2731006v1_genomic.fna/dqc_result.json
[2023-06-29 01:46:34,136] [INFO] DFAST_QC completed!
[2023-06-29 01:46:34,136] [INFO] Total running time: 0h0m25s