[2023-06-28 22:24:34,196] [INFO] DFAST_QC pipeline started.
[2023-06-28 22:24:34,198] [INFO] DFAST_QC version: 0.5.7
[2023-06-28 22:24:34,199] [INFO] DQC Reference Directory: /var/lib/cwl/stg923eec53-d2d4-4650-bedb-b3f181314910/dqc_reference
[2023-06-28 22:24:35,726] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-28 22:24:35,727] [INFO] Task started: Prodigal
[2023-06-28 22:24:35,727] [INFO] Running command: gunzip -c /var/lib/cwl/stgef07278f-4be1-4bd0-9551-386924c40394/GCA_027400255.1_ASM2740025v1_genomic.fna.gz | prodigal -d GCA_027400255.1_ASM2740025v1_genomic.fna/cds.fna -a GCA_027400255.1_ASM2740025v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-28 22:24:41,874] [INFO] Task succeeded: Prodigal
[2023-06-28 22:24:41,874] [INFO] Task started: HMMsearch
[2023-06-28 22:24:41,874] [INFO] Running command: hmmsearch --tblout GCA_027400255.1_ASM2740025v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg923eec53-d2d4-4650-bedb-b3f181314910/dqc_reference/reference_markers.hmm GCA_027400255.1_ASM2740025v1_genomic.fna/protein.faa > /dev/null
[2023-06-28 22:24:42,119] [INFO] Task succeeded: HMMsearch
[2023-06-28 22:24:42,121] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgef07278f-4be1-4bd0-9551-386924c40394/GCA_027400255.1_ASM2740025v1_genomic.fna.gz]
[2023-06-28 22:24:42,146] [INFO] Query marker FASTA was written to GCA_027400255.1_ASM2740025v1_genomic.fna/markers.fasta
[2023-06-28 22:24:42,146] [INFO] Task started: Blastn
[2023-06-28 22:24:42,146] [INFO] Running command: blastn -query GCA_027400255.1_ASM2740025v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg923eec53-d2d4-4650-bedb-b3f181314910/dqc_reference/reference_markers.fasta -out GCA_027400255.1_ASM2740025v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 22:24:42,717] [INFO] Task succeeded: Blastn
[2023-06-28 22:24:42,721] [INFO] Selected 14 target genomes.
[2023-06-28 22:24:42,722] [INFO] Target genome list was writen to GCA_027400255.1_ASM2740025v1_genomic.fna/target_genomes.txt
[2023-06-28 22:24:42,724] [INFO] Task started: fastANI
[2023-06-28 22:24:42,724] [INFO] Running command: fastANI --query /var/lib/cwl/stgef07278f-4be1-4bd0-9551-386924c40394/GCA_027400255.1_ASM2740025v1_genomic.fna.gz --refList GCA_027400255.1_ASM2740025v1_genomic.fna/target_genomes.txt --output GCA_027400255.1_ASM2740025v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-28 22:24:50,078] [INFO] Task succeeded: fastANI
[2023-06-28 22:24:50,079] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg923eec53-d2d4-4650-bedb-b3f181314910/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-28 22:24:50,079] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg923eec53-d2d4-4650-bedb-b3f181314910/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-28 22:24:50,081] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-28 22:24:50,081] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-28 22:24:50,082] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-28 22:24:50,084] [INFO] DFAST Taxonomy check result was written to GCA_027400255.1_ASM2740025v1_genomic.fna/tc_result.tsv
[2023-06-28 22:24:50,085] [INFO] ===== Taxonomy check completed =====
[2023-06-28 22:24:50,085] [INFO] ===== Start completeness check using CheckM =====
[2023-06-28 22:24:50,085] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg923eec53-d2d4-4650-bedb-b3f181314910/dqc_reference/checkm_data
[2023-06-28 22:24:50,089] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-28 22:24:50,114] [INFO] Task started: CheckM
[2023-06-28 22:24:50,114] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_027400255.1_ASM2740025v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_027400255.1_ASM2740025v1_genomic.fna/checkm_input GCA_027400255.1_ASM2740025v1_genomic.fna/checkm_result
[2023-06-28 22:25:14,646] [INFO] Task succeeded: CheckM
[2023-06-28 22:25:14,647] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-28 22:25:14,668] [INFO] ===== Completeness check finished =====
[2023-06-28 22:25:14,669] [INFO] ===== Start GTDB Search =====
[2023-06-28 22:25:14,669] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_027400255.1_ASM2740025v1_genomic.fna/markers.fasta)
[2023-06-28 22:25:14,669] [INFO] Task started: Blastn
[2023-06-28 22:25:14,670] [INFO] Running command: blastn -query GCA_027400255.1_ASM2740025v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg923eec53-d2d4-4650-bedb-b3f181314910/dqc_reference/reference_markers_gtdb.fasta -out GCA_027400255.1_ASM2740025v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 22:25:15,453] [INFO] Task succeeded: Blastn
[2023-06-28 22:25:15,459] [INFO] Selected 22 target genomes.
[2023-06-28 22:25:15,459] [INFO] Target genome list was writen to GCA_027400255.1_ASM2740025v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-28 22:25:15,470] [INFO] Task started: fastANI
[2023-06-28 22:25:15,470] [INFO] Running command: fastANI --query /var/lib/cwl/stgef07278f-4be1-4bd0-9551-386924c40394/GCA_027400255.1_ASM2740025v1_genomic.fna.gz --refList GCA_027400255.1_ASM2740025v1_genomic.fna/target_genomes_gtdb.txt --output GCA_027400255.1_ASM2740025v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-28 22:25:28,146] [INFO] Task succeeded: fastANI
[2023-06-28 22:25:28,150] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-28 22:25:28,150] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_001796355.1	s__Bellilinea sp001796355	77.8928	184	733	d__Bacteria;p__Chloroflexota;c__Anaerolineae;o__Anaerolineales;f__Anaerolineaceae;g__Bellilinea	95.0	99.55	99.40	0.87	0.85	3	-
GCA_004347695.1	s__Bellilinea sp004347695	77.6354	199	733	d__Bacteria;p__Chloroflexota;c__Anaerolineae;o__Anaerolineales;f__Anaerolineaceae;g__Bellilinea	95.0	98.75	98.75	0.96	0.96	2	-
--------------------------------------------------------------------------------
[2023-06-28 22:25:28,152] [INFO] GTDB search result was written to GCA_027400255.1_ASM2740025v1_genomic.fna/result_gtdb.tsv
[2023-06-28 22:25:28,153] [INFO] ===== GTDB Search completed =====
[2023-06-28 22:25:28,155] [INFO] DFAST_QC result json was written to GCA_027400255.1_ASM2740025v1_genomic.fna/dqc_result.json
[2023-06-28 22:25:28,155] [INFO] DFAST_QC completed!
[2023-06-28 22:25:28,156] [INFO] Total running time: 0h0m54s