[2023-06-29 09:20:16,039] [INFO] DFAST_QC pipeline started.
[2023-06-29 09:20:16,043] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 09:20:16,043] [INFO] DQC Reference Directory: /var/lib/cwl/stgbbf93c40-e01b-4557-9ad0-5941642f1a53/dqc_reference
[2023-06-29 09:20:17,558] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 09:20:17,559] [INFO] Task started: Prodigal
[2023-06-29 09:20:17,560] [INFO] Running command: gunzip -c /var/lib/cwl/stg167cfeb4-cd27-41ed-ad6b-cebf8ad096dd/GCA_027415455.1_ASM2741545v1_genomic.fna.gz | prodigal -d GCA_027415455.1_ASM2741545v1_genomic.fna/cds.fna -a GCA_027415455.1_ASM2741545v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 09:20:23,135] [INFO] Task succeeded: Prodigal
[2023-06-29 09:20:23,136] [INFO] Task started: HMMsearch
[2023-06-29 09:20:23,136] [INFO] Running command: hmmsearch --tblout GCA_027415455.1_ASM2741545v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgbbf93c40-e01b-4557-9ad0-5941642f1a53/dqc_reference/reference_markers.hmm GCA_027415455.1_ASM2741545v1_genomic.fna/protein.faa > /dev/null
[2023-06-29 09:20:23,381] [INFO] Task succeeded: HMMsearch
[2023-06-29 09:20:23,383] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg167cfeb4-cd27-41ed-ad6b-cebf8ad096dd/GCA_027415455.1_ASM2741545v1_genomic.fna.gz]
[2023-06-29 09:20:23,410] [INFO] Query marker FASTA was written to GCA_027415455.1_ASM2741545v1_genomic.fna/markers.fasta
[2023-06-29 09:20:23,410] [INFO] Task started: Blastn
[2023-06-29 09:20:23,411] [INFO] Running command: blastn -query GCA_027415455.1_ASM2741545v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbbf93c40-e01b-4557-9ad0-5941642f1a53/dqc_reference/reference_markers.fasta -out GCA_027415455.1_ASM2741545v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 09:20:24,006] [INFO] Task succeeded: Blastn
[2023-06-29 09:20:24,010] [INFO] Selected 10 target genomes.
[2023-06-29 09:20:24,010] [INFO] Target genome list was writen to GCA_027415455.1_ASM2741545v1_genomic.fna/target_genomes.txt
[2023-06-29 09:20:24,015] [INFO] Task started: fastANI
[2023-06-29 09:20:24,015] [INFO] Running command: fastANI --query /var/lib/cwl/stg167cfeb4-cd27-41ed-ad6b-cebf8ad096dd/GCA_027415455.1_ASM2741545v1_genomic.fna.gz --refList GCA_027415455.1_ASM2741545v1_genomic.fna/target_genomes.txt --output GCA_027415455.1_ASM2741545v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 09:20:30,702] [INFO] Task succeeded: fastANI
[2023-06-29 09:20:30,703] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgbbf93c40-e01b-4557-9ad0-5941642f1a53/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 09:20:30,703] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgbbf93c40-e01b-4557-9ad0-5941642f1a53/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 09:20:30,706] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 09:20:30,706] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 09:20:30,706] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 09:20:30,709] [INFO] DFAST Taxonomy check result was written to GCA_027415455.1_ASM2741545v1_genomic.fna/tc_result.tsv
[2023-06-29 09:20:30,710] [INFO] ===== Taxonomy check completed =====
[2023-06-29 09:20:30,710] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 09:20:30,710] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgbbf93c40-e01b-4557-9ad0-5941642f1a53/dqc_reference/checkm_data
[2023-06-29 09:20:30,716] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 09:20:30,750] [INFO] Task started: CheckM
[2023-06-29 09:20:30,751] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_027415455.1_ASM2741545v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_027415455.1_ASM2741545v1_genomic.fna/checkm_input GCA_027415455.1_ASM2741545v1_genomic.fna/checkm_result
[2023-06-29 09:20:53,982] [INFO] Task succeeded: CheckM
[2023-06-29 09:20:53,983] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 58.33%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 09:20:54,006] [INFO] ===== Completeness check finished =====
[2023-06-29 09:20:54,007] [INFO] ===== Start GTDB Search =====
[2023-06-29 09:20:54,007] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_027415455.1_ASM2741545v1_genomic.fna/markers.fasta)
[2023-06-29 09:20:54,008] [INFO] Task started: Blastn
[2023-06-29 09:20:54,008] [INFO] Running command: blastn -query GCA_027415455.1_ASM2741545v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbbf93c40-e01b-4557-9ad0-5941642f1a53/dqc_reference/reference_markers_gtdb.fasta -out GCA_027415455.1_ASM2741545v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 09:20:54,799] [INFO] Task succeeded: Blastn
[2023-06-29 09:20:54,813] [INFO] Selected 15 target genomes.
[2023-06-29 09:20:54,814] [INFO] Target genome list was writen to GCA_027415455.1_ASM2741545v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 09:20:54,824] [INFO] Task started: fastANI
[2023-06-29 09:20:54,824] [INFO] Running command: fastANI --query /var/lib/cwl/stg167cfeb4-cd27-41ed-ad6b-cebf8ad096dd/GCA_027415455.1_ASM2741545v1_genomic.fna.gz --refList GCA_027415455.1_ASM2741545v1_genomic.fna/target_genomes_gtdb.txt --output GCA_027415455.1_ASM2741545v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 09:21:01,759] [INFO] Task succeeded: fastANI
[2023-06-29 09:21:01,764] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-29 09:21:01,764] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002387725.1	s__UBA4572 sp002387725	78.4554	127	561	d__Bacteria;p__Nitrospirota_A;c__Leptospirillia;o__Leptospirillales;f__Leptospirillaceae;g__UBA4572	95.0	99.80	99.72	0.88	0.85	7	-
--------------------------------------------------------------------------------
[2023-06-29 09:21:01,767] [INFO] GTDB search result was written to GCA_027415455.1_ASM2741545v1_genomic.fna/result_gtdb.tsv
[2023-06-29 09:21:01,768] [INFO] ===== GTDB Search completed =====
[2023-06-29 09:21:01,770] [INFO] DFAST_QC result json was written to GCA_027415455.1_ASM2741545v1_genomic.fna/dqc_result.json
[2023-06-29 09:21:01,770] [INFO] DFAST_QC completed!
[2023-06-29 09:21:01,770] [INFO] Total running time: 0h0m46s