[2023-06-17 03:13:54,590] [INFO] DFAST_QC pipeline started.
[2023-06-17 03:13:54,604] [INFO] DFAST_QC version: 0.5.7
[2023-06-17 03:13:54,604] [INFO] DQC Reference Directory: /var/lib/cwl/stg92c13316-9931-4513-b744-4dbed4852ead/dqc_reference
[2023-06-17 03:13:55,938] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-17 03:13:55,938] [INFO] Task started: Prodigal
[2023-06-17 03:13:55,939] [INFO] Running command: gunzip -c /var/lib/cwl/stg599ca012-4082-44bd-ab85-f97e3230fc7f/GCA_028708725.1_ASM2870872v1_genomic.fna.gz | prodigal -d GCA_028708725.1_ASM2870872v1_genomic.fna/cds.fna -a GCA_028708725.1_ASM2870872v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-17 03:13:59,474] [INFO] Task succeeded: Prodigal
[2023-06-17 03:13:59,475] [INFO] Task started: HMMsearch
[2023-06-17 03:13:59,475] [INFO] Running command: hmmsearch --tblout GCA_028708725.1_ASM2870872v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg92c13316-9931-4513-b744-4dbed4852ead/dqc_reference/reference_markers.hmm GCA_028708725.1_ASM2870872v1_genomic.fna/protein.faa > /dev/null
[2023-06-17 03:13:59,727] [INFO] Task succeeded: HMMsearch
[2023-06-17 03:13:59,729] [INFO] Found 6/6 markers.
[2023-06-17 03:13:59,747] [INFO] Query marker FASTA was written to GCA_028708725.1_ASM2870872v1_genomic.fna/markers.fasta
[2023-06-17 03:13:59,747] [INFO] Task started: Blastn
[2023-06-17 03:13:59,747] [INFO] Running command: blastn -query GCA_028708725.1_ASM2870872v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg92c13316-9931-4513-b744-4dbed4852ead/dqc_reference/reference_markers.fasta -out GCA_028708725.1_ASM2870872v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-17 03:14:00,327] [INFO] Task succeeded: Blastn
[2023-06-17 03:14:00,332] [INFO] Selected 20 target genomes.
[2023-06-17 03:14:00,332] [INFO] Target genome list was writen to GCA_028708725.1_ASM2870872v1_genomic.fna/target_genomes.txt
[2023-06-17 03:14:00,347] [INFO] Task started: fastANI
[2023-06-17 03:14:00,347] [INFO] Running command: fastANI --query /var/lib/cwl/stg599ca012-4082-44bd-ab85-f97e3230fc7f/GCA_028708725.1_ASM2870872v1_genomic.fna.gz --refList GCA_028708725.1_ASM2870872v1_genomic.fna/target_genomes.txt --output GCA_028708725.1_ASM2870872v1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-17 03:14:07,556] [INFO] Task succeeded: fastANI
[2023-06-17 03:14:07,556] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg92c13316-9931-4513-b744-4dbed4852ead/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-17 03:14:07,557] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg92c13316-9931-4513-b744-4dbed4852ead/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-17 03:14:07,559] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-17 03:14:07,559] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-17 03:14:07,559] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-17 03:14:07,561] [INFO] DFAST Taxonomy check result was written to GCA_028708725.1_ASM2870872v1_genomic.fna/tc_result.tsv
[2023-06-17 03:14:07,562] [INFO] ===== Taxonomy check completed =====
[2023-06-17 03:14:07,562] [INFO] ===== Start completeness check using CheckM =====
[2023-06-17 03:14:07,563] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg92c13316-9931-4513-b744-4dbed4852ead/dqc_reference/checkm_data
[2023-06-17 03:14:07,566] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-17 03:14:07,588] [INFO] Task started: CheckM
[2023-06-17 03:14:07,588] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_028708725.1_ASM2870872v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_028708725.1_ASM2870872v1_genomic.fna/checkm_input GCA_028708725.1_ASM2870872v1_genomic.fna/checkm_result
[2023-06-17 03:14:26,983] [INFO] Task succeeded: CheckM
[2023-06-17 03:14:26,984] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-17 03:14:27,008] [INFO] ===== Completeness check finished =====
[2023-06-17 03:14:27,008] [INFO] ===== Start GTDB Search =====
[2023-06-17 03:14:27,009] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_028708725.1_ASM2870872v1_genomic.fna/markers.fasta)
[2023-06-17 03:14:27,009] [INFO] Task started: Blastn
[2023-06-17 03:14:27,009] [INFO] Running command: blastn -query GCA_028708725.1_ASM2870872v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg92c13316-9931-4513-b744-4dbed4852ead/dqc_reference/reference_markers_gtdb.fasta -out GCA_028708725.1_ASM2870872v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-17 03:14:27,829] [INFO] Task succeeded: Blastn
[2023-06-17 03:14:27,834] [INFO] Selected 20 target genomes.
[2023-06-17 03:14:27,835] [INFO] Target genome list was writen to GCA_028708725.1_ASM2870872v1_genomic.fna/target_genomes_gtdb.txt
[2023-06-17 03:14:27,859] [INFO] Task started: fastANI
[2023-06-17 03:14:27,860] [INFO] Running command: fastANI --query /var/lib/cwl/stg599ca012-4082-44bd-ab85-f97e3230fc7f/GCA_028708725.1_ASM2870872v1_genomic.fna.gz --refList GCA_028708725.1_ASM2870872v1_genomic.fna/target_genomes_gtdb.txt --output GCA_028708725.1_ASM2870872v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-17 03:14:32,432] [INFO] Task succeeded: fastANI
[2023-06-17 03:14:32,436] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-17 03:14:32,436] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_019248485.1	s__S5-K13 sp019248485	77.9672	66	353	d__Bacteria;p__Patescibacteria;c__ABY1;o__UBA1558;f__S5-K13;g__S5-K13	95.0	99.71	99.48	0.86	0.78	3	-
--------------------------------------------------------------------------------
[2023-06-17 03:14:32,438] [INFO] GTDB search result was written to GCA_028708725.1_ASM2870872v1_genomic.fna/result_gtdb.tsv
[2023-06-17 03:14:32,439] [INFO] ===== GTDB Search completed =====
[2023-06-17 03:14:32,442] [INFO] DFAST_QC result json was written to GCA_028708725.1_ASM2870872v1_genomic.fna/dqc_result.json
[2023-06-17 03:14:32,443] [INFO] DFAST_QC completed!
[2023-06-17 03:14:32,443] [INFO] Total running time: 0h0m38s