[2023-06-29 22:57:27,535] [INFO] DFAST_QC pipeline started.
[2023-06-29 22:57:27,537] [INFO] DFAST_QC version: 0.5.7
[2023-06-29 22:57:27,537] [INFO] DQC Reference Directory: /var/lib/cwl/stg7a98a1ab-5368-47ec-bf0f-edd2f30b79d2/dqc_reference
[2023-06-29 22:57:28,850] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-29 22:57:28,851] [INFO] Task started: Prodigal
[2023-06-29 22:57:28,852] [INFO] Running command: gunzip -c /var/lib/cwl/stg7c1a348d-2757-47db-87d4-8ea69f322e18/GCA_900768155.1_SRS476076_133_genomic.fna.gz | prodigal -d GCA_900768155.1_SRS476076_133_genomic.fna/cds.fna -a GCA_900768155.1_SRS476076_133_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-29 22:57:33,963] [INFO] Task succeeded: Prodigal
[2023-06-29 22:57:33,963] [INFO] Task started: HMMsearch
[2023-06-29 22:57:33,964] [INFO] Running command: hmmsearch --tblout GCA_900768155.1_SRS476076_133_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg7a98a1ab-5368-47ec-bf0f-edd2f30b79d2/dqc_reference/reference_markers.hmm GCA_900768155.1_SRS476076_133_genomic.fna/protein.faa > /dev/null
[2023-06-29 22:57:34,193] [INFO] Task succeeded: HMMsearch
[2023-06-29 22:57:34,194] [INFO] Found 6/6 markers.
[2023-06-29 22:57:34,221] [INFO] Query marker FASTA was written to GCA_900768155.1_SRS476076_133_genomic.fna/markers.fasta
[2023-06-29 22:57:34,221] [INFO] Task started: Blastn
[2023-06-29 22:57:34,221] [INFO] Running command: blastn -query GCA_900768155.1_SRS476076_133_genomic.fna/markers.fasta -db /var/lib/cwl/stg7a98a1ab-5368-47ec-bf0f-edd2f30b79d2/dqc_reference/reference_markers.fasta -out GCA_900768155.1_SRS476076_133_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 22:57:34,799] [INFO] Task succeeded: Blastn
[2023-06-29 22:57:34,804] [INFO] Selected 23 target genomes.
[2023-06-29 22:57:34,805] [INFO] Target genome list was writen to GCA_900768155.1_SRS476076_133_genomic.fna/target_genomes.txt
[2023-06-29 22:57:34,812] [INFO] Task started: fastANI
[2023-06-29 22:57:34,812] [INFO] Running command: fastANI --query /var/lib/cwl/stg7c1a348d-2757-47db-87d4-8ea69f322e18/GCA_900768155.1_SRS476076_133_genomic.fna.gz --refList GCA_900768155.1_SRS476076_133_genomic.fna/target_genomes.txt --output GCA_900768155.1_SRS476076_133_genomic.fna/fastani_result.tsv --threads 1
[2023-06-29 22:57:50,262] [INFO] Task succeeded: fastANI
[2023-06-29 22:57:50,263] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg7a98a1ab-5368-47ec-bf0f-edd2f30b79d2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-29 22:57:50,263] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg7a98a1ab-5368-47ec-bf0f-edd2f30b79d2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-29 22:57:50,265] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-29 22:57:50,265] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-29 22:57:50,265] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-29 22:57:50,268] [INFO] DFAST Taxonomy check result was written to GCA_900768155.1_SRS476076_133_genomic.fna/tc_result.tsv
[2023-06-29 22:57:50,269] [INFO] ===== Taxonomy check completed =====
[2023-06-29 22:57:50,269] [INFO] ===== Start completeness check using CheckM =====
[2023-06-29 22:57:50,269] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg7a98a1ab-5368-47ec-bf0f-edd2f30b79d2/dqc_reference/checkm_data
[2023-06-29 22:57:50,273] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-29 22:57:50,304] [INFO] Task started: CheckM
[2023-06-29 22:57:50,304] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_900768155.1_SRS476076_133_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_900768155.1_SRS476076_133_genomic.fna/checkm_input GCA_900768155.1_SRS476076_133_genomic.fna/checkm_result
[2023-06-29 22:58:12,416] [INFO] Task succeeded: CheckM
[2023-06-29 22:58:12,418] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-29 22:58:12,439] [INFO] ===== Completeness check finished =====
[2023-06-29 22:58:12,439] [INFO] ===== Start GTDB Search =====
[2023-06-29 22:58:12,439] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_900768155.1_SRS476076_133_genomic.fna/markers.fasta)
[2023-06-29 22:58:12,440] [INFO] Task started: Blastn
[2023-06-29 22:58:12,440] [INFO] Running command: blastn -query GCA_900768155.1_SRS476076_133_genomic.fna/markers.fasta -db /var/lib/cwl/stg7a98a1ab-5368-47ec-bf0f-edd2f30b79d2/dqc_reference/reference_markers_gtdb.fasta -out GCA_900768155.1_SRS476076_133_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-29 22:58:13,193] [INFO] Task succeeded: Blastn
[2023-06-29 22:58:13,201] [INFO] Selected 20 target genomes.
[2023-06-29 22:58:13,201] [INFO] Target genome list was writen to GCA_900768155.1_SRS476076_133_genomic.fna/target_genomes_gtdb.txt
[2023-06-29 22:58:13,221] [INFO] Task started: fastANI
[2023-06-29 22:58:13,221] [INFO] Running command: fastANI --query /var/lib/cwl/stg7c1a348d-2757-47db-87d4-8ea69f322e18/GCA_900768155.1_SRS476076_133_genomic.fna.gz --refList GCA_900768155.1_SRS476076_133_genomic.fna/target_genomes_gtdb.txt --output GCA_900768155.1_SRS476076_133_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-29 22:58:19,380] [INFO] Task succeeded: fastANI
[2023-06-29 22:58:19,385] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-29 22:58:19,386] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_900544545.1	s__UMGS692 sp900544545	99.2275	515	759	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Christensenellales;f__CAG-314;g__UMGS692	95.0	99.05	98.86	0.89	0.85	3	conclusive
--------------------------------------------------------------------------------
[2023-06-29 22:58:19,388] [INFO] GTDB search result was written to GCA_900768155.1_SRS476076_133_genomic.fna/result_gtdb.tsv
[2023-06-29 22:58:19,389] [INFO] ===== GTDB Search completed =====
[2023-06-29 22:58:19,392] [INFO] DFAST_QC result json was written to GCA_900768155.1_SRS476076_133_genomic.fna/dqc_result.json
[2023-06-29 22:58:19,392] [INFO] DFAST_QC completed!
[2023-06-29 22:58:19,392] [INFO] Total running time: 0h0m52s