[2023-06-08 12:44:36,945] [INFO] DFAST_QC pipeline started.
[2023-06-08 12:44:36,949] [INFO] DFAST_QC version: 0.5.7
[2023-06-08 12:44:36,949] [INFO] DQC Reference Directory: /var/lib/cwl/stg8e7a4cb4-8b93-4f4a-ae79-6c4d6a4b7568/dqc_reference
[2023-06-08 12:44:38,371] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-08 12:44:38,372] [INFO] Task started: Prodigal
[2023-06-08 12:44:38,373] [INFO] Running command: gunzip -c /var/lib/cwl/stg95701be1-5ec7-437a-a08f-ed8e7e7b998b/GCA_902526045.1_AG-337-I17_genomic.fna.gz | prodigal -d GCA_902526045.1_AG-337-I17_genomic.fna/cds.fna -a GCA_902526045.1_AG-337-I17_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-08 12:44:40,743] [INFO] Task succeeded: Prodigal
[2023-06-08 12:44:40,744] [INFO] Task started: HMMsearch
[2023-06-08 12:44:40,744] [INFO] Running command: hmmsearch --tblout GCA_902526045.1_AG-337-I17_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8e7a4cb4-8b93-4f4a-ae79-6c4d6a4b7568/dqc_reference/reference_markers.hmm GCA_902526045.1_AG-337-I17_genomic.fna/protein.faa > /dev/null
[2023-06-08 12:44:41,005] [INFO] Task succeeded: HMMsearch
[2023-06-08 12:44:41,006] [INFO] Found 6/6 markers.
[2023-06-08 12:44:41,036] [INFO] Query marker FASTA was written to GCA_902526045.1_AG-337-I17_genomic.fna/markers.fasta
[2023-06-08 12:44:41,036] [INFO] Task started: Blastn
[2023-06-08 12:44:41,036] [INFO] Running command: blastn -query GCA_902526045.1_AG-337-I17_genomic.fna/markers.fasta -db /var/lib/cwl/stg8e7a4cb4-8b93-4f4a-ae79-6c4d6a4b7568/dqc_reference/reference_markers.fasta -out GCA_902526045.1_AG-337-I17_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-08 12:44:41,708] [INFO] Task succeeded: Blastn
[2023-06-08 12:44:41,712] [INFO] Selected 18 target genomes.
[2023-06-08 12:44:41,712] [INFO] Target genome list was writen to GCA_902526045.1_AG-337-I17_genomic.fna/target_genomes.txt
[2023-06-08 12:44:41,713] [INFO] Task started: fastANI
[2023-06-08 12:44:41,713] [INFO] Running command: fastANI --query /var/lib/cwl/stg95701be1-5ec7-437a-a08f-ed8e7e7b998b/GCA_902526045.1_AG-337-I17_genomic.fna.gz --refList GCA_902526045.1_AG-337-I17_genomic.fna/target_genomes.txt --output GCA_902526045.1_AG-337-I17_genomic.fna/fastani_result.tsv --threads 1
[2023-06-08 12:44:51,697] [INFO] Task succeeded: fastANI
[2023-06-08 12:44:51,698] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8e7a4cb4-8b93-4f4a-ae79-6c4d6a4b7568/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-08 12:44:51,698] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8e7a4cb4-8b93-4f4a-ae79-6c4d6a4b7568/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-08 12:44:51,700] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-08 12:44:51,700] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-08 12:44:51,700] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-08 12:44:51,702] [INFO] DFAST Taxonomy check result was written to GCA_902526045.1_AG-337-I17_genomic.fna/tc_result.tsv
[2023-06-08 12:44:51,703] [INFO] ===== Taxonomy check completed =====
[2023-06-08 12:44:51,703] [INFO] ===== Start completeness check using CheckM =====
[2023-06-08 12:44:51,703] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8e7a4cb4-8b93-4f4a-ae79-6c4d6a4b7568/dqc_reference/checkm_data
[2023-06-08 12:44:51,706] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-08 12:44:51,721] [INFO] Task started: CheckM
[2023-06-08 12:44:51,721] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_902526045.1_AG-337-I17_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_902526045.1_AG-337-I17_genomic.fna/checkm_input GCA_902526045.1_AG-337-I17_genomic.fna/checkm_result
[2023-06-08 12:45:07,427] [INFO] Task succeeded: CheckM
[2023-06-08 12:45:07,431] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-08 12:45:07,458] [INFO] ===== Completeness check finished =====
[2023-06-08 12:45:07,458] [INFO] ===== Start GTDB Search =====
[2023-06-08 12:45:07,459] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_902526045.1_AG-337-I17_genomic.fna/markers.fasta)
[2023-06-08 12:45:07,459] [INFO] Task started: Blastn
[2023-06-08 12:45:07,459] [INFO] Running command: blastn -query GCA_902526045.1_AG-337-I17_genomic.fna/markers.fasta -db /var/lib/cwl/stg8e7a4cb4-8b93-4f4a-ae79-6c4d6a4b7568/dqc_reference/reference_markers_gtdb.fasta -out GCA_902526045.1_AG-337-I17_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-08 12:45:08,445] [INFO] Task succeeded: Blastn
[2023-06-08 12:45:08,449] [INFO] Selected 8 target genomes.
[2023-06-08 12:45:08,449] [INFO] Target genome list was writen to GCA_902526045.1_AG-337-I17_genomic.fna/target_genomes_gtdb.txt
[2023-06-08 12:45:08,454] [INFO] Task started: fastANI
[2023-06-08 12:45:08,455] [INFO] Running command: fastANI --query /var/lib/cwl/stg95701be1-5ec7-437a-a08f-ed8e7e7b998b/GCA_902526045.1_AG-337-I17_genomic.fna.gz --refList GCA_902526045.1_AG-337-I17_genomic.fna/target_genomes_gtdb.txt --output GCA_902526045.1_AG-337-I17_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-08 12:45:10,623] [INFO] Task succeeded: fastANI
[2023-06-08 12:45:10,631] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-08 12:45:10,631] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_902526045.1	s__CACNYO01 sp902526045	100.0	392	393	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__AG-339-G14;g__CACNYO01	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_902528485.1	s__CACNYO01 sp902528485	92.0803	221	393	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__AG-339-G14;g__CACNYO01	95.0	95.61	95.61	0.68	0.68	2	-
GCA_902589965.1	s__CACNYO01 sp902589965	87.4183	292	393	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__AG-339-G14;g__CACNYO01	95.0	95.54	95.01	0.78	0.76	10	-
GCA_902596665.1	s__CACNYO01 sp902596665	87.3493	319	393	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__AG-339-G14;g__CACNYO01	95.0	95.60	95.38	0.89	0.88	7	-
GCA_902570035.1	s__CACNYO01 sp902570035	87.2506	304	393	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__AG-339-G14;g__CACNYO01	95.0	96.39	95.41	0.82	0.78	11	-
GCA_902594855.1	s__CACNYO01 sp902594855	87.0158	303	393	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__AG-339-G14;g__CACNYO01	95.0	96.60	95.51	0.88	0.82	11	-
GCA_902578495.1	s__CACNYO01 sp902578495	86.9043	298	393	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__AG-339-G14;g__CACNYO01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902624765.1	s__CACNYO01 sp902624765	85.8229	221	393	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__AG-339-G14;g__CACNYO01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-08 12:45:10,633] [INFO] GTDB search result was written to GCA_902526045.1_AG-337-I17_genomic.fna/result_gtdb.tsv
[2023-06-08 12:45:10,634] [INFO] ===== GTDB Search completed =====
[2023-06-08 12:45:10,637] [INFO] DFAST_QC result json was written to GCA_902526045.1_AG-337-I17_genomic.fna/dqc_result.json
[2023-06-08 12:45:10,637] [INFO] DFAST_QC completed!
[2023-06-08 12:45:10,637] [INFO] Total running time: 0h0m34s