[2023-06-08 06:40:23,899] [INFO] DFAST_QC pipeline started.
[2023-06-08 06:40:23,901] [INFO] DFAST_QC version: 0.5.7
[2023-06-08 06:40:23,902] [INFO] DQC Reference Directory: /var/lib/cwl/stga16b4eb6-79da-4e57-b82d-c567dd235f41/dqc_reference
[2023-06-08 06:40:25,076] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-08 06:40:25,077] [INFO] Task started: Prodigal
[2023-06-08 06:40:25,077] [INFO] Running command: gunzip -c /var/lib/cwl/stg63155cea-8573-46bd-8d04-b3c02f401643/GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna.gz | prodigal -d GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/cds.fna -a GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-08 06:40:37,243] [INFO] Task succeeded: Prodigal
[2023-06-08 06:40:37,243] [INFO] Task started: HMMsearch
[2023-06-08 06:40:37,243] [INFO] Running command: hmmsearch --tblout GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga16b4eb6-79da-4e57-b82d-c567dd235f41/dqc_reference/reference_markers.hmm GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/protein.faa > /dev/null
[2023-06-08 06:40:37,454] [INFO] Task succeeded: HMMsearch
[2023-06-08 06:40:37,456] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg63155cea-8573-46bd-8d04-b3c02f401643/GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna.gz]
[2023-06-08 06:40:37,481] [INFO] Query marker FASTA was written to GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/markers.fasta
[2023-06-08 06:40:37,482] [INFO] Task started: Blastn
[2023-06-08 06:40:37,482] [INFO] Running command: blastn -query GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/markers.fasta -db /var/lib/cwl/stga16b4eb6-79da-4e57-b82d-c567dd235f41/dqc_reference/reference_markers.fasta -out GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-08 06:40:38,054] [INFO] Task succeeded: Blastn
[2023-06-08 06:40:38,059] [INFO] Selected 24 target genomes.
[2023-06-08 06:40:38,059] [INFO] Target genome list was writen to GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/target_genomes.txt
[2023-06-08 06:40:38,061] [INFO] Task started: fastANI
[2023-06-08 06:40:38,061] [INFO] Running command: fastANI --query /var/lib/cwl/stg63155cea-8573-46bd-8d04-b3c02f401643/GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna.gz --refList GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/target_genomes.txt --output GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/fastani_result.tsv --threads 1
[2023-06-08 06:40:49,665] [INFO] Task succeeded: fastANI
[2023-06-08 06:40:49,666] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga16b4eb6-79da-4e57-b82d-c567dd235f41/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-08 06:40:49,667] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga16b4eb6-79da-4e57-b82d-c567dd235f41/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-08 06:40:49,689] [INFO] Found 14 fastANI hits (0 hits with ANI > threshold)
[2023-06-08 06:40:49,690] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-06-08 06:40:49,690] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Prevotella stercorea	strain=DSM 18206	GCA_000235885.1	363265	363265	suspected-type	True	77.4532	101	567	95	below_threshold
Prevotella dentalis	strain=DSM 3688	GCA_000242335.3	52227	52227	type	True	77.0275	118	567	95	below_threshold
Prevotella dentalis	strain=DSM 3688	GCA_000220215.1	52227	52227	type	True	76.9877	120	567	95	below_threshold
Hallella seregens	strain=ATCC 51272	GCA_000518545.1	52229	52229	type	True	76.9625	109	567	95	below_threshold
Prevotella buccae	strain=ATCC 33574	GCA_000184945.1	28126	28126	type	True	76.7532	115	567	95	below_threshold
Prevotella baroniae	strain=JCM 13447	GCA_000613565.1	305719	305719	type	True	76.6872	84	567	95	below_threshold
Prevotella baroniae	strain=DSM 16972	GCA_000426585.1	305719	305719	type	True	76.6866	83	567	95	below_threshold
Prevotella pectinovora	strain=P4-76	GCA_000834015.1	1602169	1602169	type	True	76.6577	90	567	95	below_threshold
Prevotella multiformis	strain=DSM 16608	GCA_000191065.1	282402	282402	type	True	76.647	81	567	95	below_threshold
Hallella faecis	strain=CLA-AA-H145	GCA_018789675.1	2841596	2841596	type	True	76.6181	98	567	95	below_threshold
Prevotella lascolaii	strain=khD1	GCA_900079775.1	1776379	1776379	type	True	76.5905	81	567	95	below_threshold
Prevotella mizrahii	strain=LKV-178-WT-2A	GCA_009695775.1	2606637	2606637	type	True	76.3324	61	567	95	below_threshold
Prevotella denticola	strain=NCTC13067	GCA_900454835.1	28129	28129	type	True	76.2699	75	567	95	below_threshold
Prevotella denticola	strain=DSM 20614	GCA_000421205.1	28129	28129	type	True	76.2432	76	567	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-08 06:40:49,692] [INFO] DFAST Taxonomy check result was written to GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/tc_result.tsv
[2023-06-08 06:40:49,693] [INFO] ===== Taxonomy check completed =====
[2023-06-08 06:40:49,693] [INFO] ===== Start completeness check using CheckM =====
[2023-06-08 06:40:49,693] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga16b4eb6-79da-4e57-b82d-c567dd235f41/dqc_reference/checkm_data
[2023-06-08 06:40:49,694] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-08 06:40:49,722] [INFO] Task started: CheckM
[2023-06-08 06:40:49,722] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/checkm_input GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/checkm_result
[2023-06-08 06:41:27,064] [INFO] Task succeeded: CheckM
[2023-06-08 06:41:27,071] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 66.67%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-08 06:41:27,093] [INFO] ===== Completeness check finished =====
[2023-06-08 06:41:27,093] [INFO] ===== Start GTDB Search =====
[2023-06-08 06:41:27,094] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/markers.fasta)
[2023-06-08 06:41:27,094] [INFO] Task started: Blastn
[2023-06-08 06:41:27,094] [INFO] Running command: blastn -query GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/markers.fasta -db /var/lib/cwl/stga16b4eb6-79da-4e57-b82d-c567dd235f41/dqc_reference/reference_markers_gtdb.fasta -out GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-08 06:41:27,924] [INFO] Task succeeded: Blastn
[2023-06-08 06:41:27,929] [INFO] Selected 24 target genomes.
[2023-06-08 06:41:27,929] [INFO] Target genome list was writen to GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/target_genomes_gtdb.txt
[2023-06-08 06:41:27,932] [INFO] Task started: fastANI
[2023-06-08 06:41:27,932] [INFO] Running command: fastANI --query /var/lib/cwl/stg63155cea-8573-46bd-8d04-b3c02f401643/GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna.gz --refList GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/target_genomes_gtdb.txt --output GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-08 06:41:40,224] [INFO] Task succeeded: fastANI
[2023-06-08 06:41:40,247] [INFO] Found 24 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-08 06:41:40,247] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_900318625.1	s__Prevotella sp900318625	98.176	492	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.97	97.03	0.86	0.78	43	conclusive
GCA_002353555.1	s__Prevotella sp002353555	77.8283	133	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.61	97.57	0.79	0.77	3	-
GCA_902775475.1	s__Prevotella sp902775475	77.7514	165	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	98.69	98.51	0.89	0.87	7	-
GCA_902776685.1	s__Prevotella sp902776685	77.5553	144	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900316285.1	s__Prevotella sp900316285	77.5254	134	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	96.02	95.37	0.87	0.79	21	-
GCA_900320585.1	s__Prevotella sp900320585	77.4147	131	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	98.77	98.44	0.87	0.81	6	-
GCF_002251435.1	s__Prevotella sp002251435	77.3119	57	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_019052365.1	s__Prevotella sp000434975	77.307	106	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.22	96.59	0.82	0.74	11	-
GCA_902771785.1	s__Prevotella sp902771785	77.2928	145	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	98.01	98.01	0.87	0.87	2	-
GCA_905233825.1	s__Prevotella sp905233825	77.2531	94	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902760315.1	s__Prevotella sp902760315	77.1515	100	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002392835.1	s__Prevotella sp002392835	77.0496	116	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.57	97.57	0.74	0.74	2	-
GCA_900554695.1	s__Prevotella sp900554695	77.0403	75	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.93	97.88	0.84	0.83	3	-
GCA_900316295.1	s__Prevotella sp900316295	76.9425	96	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	98.23	97.08	0.85	0.70	10	-
GCA_017615355.1	s__Prevotella sp017615355	76.9356	109	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905233425.1	s__Prevotella sp905233425	76.915	153	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900317305.1	s__Prevotella sp900317305	76.8954	145	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	98.38	97.82	0.87	0.83	11	-
GCA_900544495.1	s__Prevotella sp900544495	76.7499	87	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	98.45	98.42	0.86	0.82	3	-
GCA_900549175.1	s__Prevotella sp900549175	76.7166	128	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	99.84	99.84	0.89	0.89	2	-
GCA_900546575.1	s__Prevotella sp900546575	76.7164	93	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	96.21	95.49	0.86	0.86	4	-
GCA_902792685.1	s__Prevotella sp902792685	76.7073	87	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	96.79	96.68	0.73	0.71	4	-
GCF_000426585.1	s__Prevotella baroniae	76.6569	82	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	98.79	98.01	0.95	0.92	4	-
GCA_900199655.1	s__Prevotella sp900199655	76.58	119	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	99.91	99.91	0.98	0.98	2	-
GCF_009695775.1	s__Prevotella mizrahii	76.3324	61	567	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.15	96.87	0.87	0.84	6	-
--------------------------------------------------------------------------------
[2023-06-08 06:41:40,249] [INFO] GTDB search result was written to GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/result_gtdb.tsv
[2023-06-08 06:41:40,249] [INFO] ===== GTDB Search completed =====
[2023-06-08 06:41:40,253] [INFO] DFAST_QC result json was written to GCA_902757985.1_Rumen_uncultured_genome_RUG10324_genomic.fna/dqc_result.json
[2023-06-08 06:41:40,254] [INFO] DFAST_QC completed!
[2023-06-08 06:41:40,254] [INFO] Total running time: 0h1m16s