[2023-06-05 13:58:14,749] [INFO] DFAST_QC pipeline started.
[2023-06-05 13:58:14,753] [INFO] DFAST_QC version: 0.5.7
[2023-06-05 13:58:14,753] [INFO] DQC Reference Directory: /var/lib/cwl/stgeeaf77ff-8597-4af4-829f-b56843973104/dqc_reference
[2023-06-05 13:58:16,324] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-05 13:58:16,325] [INFO] Task started: Prodigal
[2023-06-05 13:58:16,326] [INFO] Running command: gunzip -c /var/lib/cwl/stgc444c5a8-7bab-481b-8251-cb8d1649eeee/GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna.gz | prodigal -d GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/cds.fna -a GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-05 13:58:19,797] [INFO] Task succeeded: Prodigal
[2023-06-05 13:58:19,797] [INFO] Task started: HMMsearch
[2023-06-05 13:58:19,798] [INFO] Running command: hmmsearch --tblout GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgeeaf77ff-8597-4af4-829f-b56843973104/dqc_reference/reference_markers.hmm GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/protein.faa > /dev/null
[2023-06-05 13:58:19,964] [INFO] Task succeeded: HMMsearch
[2023-06-05 13:58:19,965] [INFO] Found 6/6 markers.
[2023-06-05 13:58:19,983] [INFO] Query marker FASTA was written to GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/markers.fasta
[2023-06-05 13:58:19,983] [INFO] Task started: Blastn
[2023-06-05 13:58:19,983] [INFO] Running command: blastn -query GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/markers.fasta -db /var/lib/cwl/stgeeaf77ff-8597-4af4-829f-b56843973104/dqc_reference/reference_markers.fasta -out GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 13:58:20,594] [INFO] Task succeeded: Blastn
[2023-06-05 13:58:20,597] [INFO] Selected 26 target genomes.
[2023-06-05 13:58:20,597] [INFO] Target genome list was writen to GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/target_genomes.txt
[2023-06-05 13:58:20,600] [INFO] Task started: fastANI
[2023-06-05 13:58:20,600] [INFO] Running command: fastANI --query /var/lib/cwl/stgc444c5a8-7bab-481b-8251-cb8d1649eeee/GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna.gz --refList GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/target_genomes.txt --output GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/fastani_result.tsv --threads 1
[2023-06-05 13:58:34,566] [INFO] Task succeeded: fastANI
[2023-06-05 13:58:34,566] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgeeaf77ff-8597-4af4-829f-b56843973104/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-05 13:58:34,566] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgeeaf77ff-8597-4af4-829f-b56843973104/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-05 13:58:34,568] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-05 13:58:34,568] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-05 13:58:34,568] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-05 13:58:34,569] [INFO] DFAST Taxonomy check result was written to GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/tc_result.tsv
[2023-06-05 13:58:34,570] [INFO] ===== Taxonomy check completed =====
[2023-06-05 13:58:34,570] [INFO] ===== Start completeness check using CheckM =====
[2023-06-05 13:58:34,570] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgeeaf77ff-8597-4af4-829f-b56843973104/dqc_reference/checkm_data
[2023-06-05 13:58:34,573] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-05 13:58:34,593] [INFO] Task started: CheckM
[2023-06-05 13:58:34,593] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/checkm_input GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/checkm_result
[2023-06-05 13:58:50,372] [INFO] Task succeeded: CheckM
[2023-06-05 13:58:50,373] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 61.81%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-05 13:58:50,387] [INFO] ===== Completeness check finished =====
[2023-06-05 13:58:50,387] [INFO] ===== Start GTDB Search =====
[2023-06-05 13:58:50,388] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/markers.fasta)
[2023-06-05 13:58:50,388] [INFO] Task started: Blastn
[2023-06-05 13:58:50,388] [INFO] Running command: blastn -query GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/markers.fasta -db /var/lib/cwl/stgeeaf77ff-8597-4af4-829f-b56843973104/dqc_reference/reference_markers_gtdb.fasta -out GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 13:58:51,474] [INFO] Task succeeded: Blastn
[2023-06-05 13:58:51,477] [INFO] Selected 13 target genomes.
[2023-06-05 13:58:51,477] [INFO] Target genome list was writen to GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/target_genomes_gtdb.txt
[2023-06-05 13:58:51,480] [INFO] Task started: fastANI
[2023-06-05 13:58:51,480] [INFO] Running command: fastANI --query /var/lib/cwl/stgc444c5a8-7bab-481b-8251-cb8d1649eeee/GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna.gz --refList GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/target_genomes_gtdb.txt --output GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-05 13:58:56,966] [INFO] Task succeeded: fastANI
[2023-06-05 13:58:56,975] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-05 13:58:56,975] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_900317315.1	s__Ruminococcus_E sp900317315	98.933	367	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	98.84	97.27	0.87	0.80	119	conclusive
GCA_902784855.1	s__Ruminococcus_E sp902784855	81.7614	277	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902799325.1	s__Ruminococcus_E sp902799325	81.6083	255	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900316815.1	s__Ruminococcus_E sp900316815	81.5799	233	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	99.08	98.49	0.85	0.78	7	-
GCA_902794395.1	s__Ruminococcus_E sp902794395	80.3144	254	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900317595.1	s__Ruminococcus_E sp900317595	80.0252	223	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	99.55	99.10	0.91	0.83	3	-
GCA_902797525.1	s__Ruminococcus_E sp902797525	80.0023	197	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902776375.1	s__Ruminococcus_E sp902776375	79.9538	254	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900100595.1	s__Ruminococcus_E sp900100595	79.5036	218	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	98.87	97.87	0.91	0.78	34	-
GCA_902761375.1	s__Ruminococcus_E sp902761375	79.361	165	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900320415.1	s__Ruminococcus_E sp900320415	78.9154	133	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	99.69	99.69	0.84	0.84	2	-
GCA_900315195.1	s__Ruminococcus_E sp900315195	78.5987	182	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	99.59	99.19	0.95	0.90	3	-
GCF_016680995.1	s__Ruminococcus_E sp016680995	78.5222	135	405	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__Ruminococcus_E	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-05 13:58:56,977] [INFO] GTDB search result was written to GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/result_gtdb.tsv
[2023-06-05 13:58:56,977] [INFO] ===== GTDB Search completed =====
[2023-06-05 13:58:56,980] [INFO] DFAST_QC result json was written to GCA_902769655.1_Rumen_uncultured_genome_RUG11491_genomic.fna/dqc_result.json
[2023-06-05 13:58:56,980] [INFO] DFAST_QC completed!
[2023-06-05 13:58:56,980] [INFO] Total running time: 0h0m42s