[2023-06-04 21:50:06,378] [INFO] DFAST_QC pipeline started.
[2023-06-04 21:50:06,380] [INFO] DFAST_QC version: 0.5.7
[2023-06-04 21:50:06,380] [INFO] DQC Reference Directory: /var/lib/cwl/stgfec2fd97-ed22-44f4-834e-59eff3dbc71c/dqc_reference
[2023-06-04 21:50:08,332] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-04 21:50:08,333] [INFO] Task started: Prodigal
[2023-06-04 21:50:08,333] [INFO] Running command: gunzip -c /var/lib/cwl/stga9e272c8-5226-4336-b9e1-1e0c0dd9f0a6/GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna.gz | prodigal -d GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/cds.fna -a GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-04 21:50:23,787] [INFO] Task succeeded: Prodigal
[2023-06-04 21:50:23,788] [INFO] Task started: HMMsearch
[2023-06-04 21:50:23,788] [INFO] Running command: hmmsearch --tblout GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfec2fd97-ed22-44f4-834e-59eff3dbc71c/dqc_reference/reference_markers.hmm GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/protein.faa > /dev/null
[2023-06-04 21:50:24,017] [INFO] Task succeeded: HMMsearch
[2023-06-04 21:50:24,019] [INFO] Found 6/6 markers.
[2023-06-04 21:50:24,047] [INFO] Query marker FASTA was written to GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/markers.fasta
[2023-06-04 21:50:24,048] [INFO] Task started: Blastn
[2023-06-04 21:50:24,048] [INFO] Running command: blastn -query GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/markers.fasta -db /var/lib/cwl/stgfec2fd97-ed22-44f4-834e-59eff3dbc71c/dqc_reference/reference_markers.fasta -out GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-04 21:50:24,648] [INFO] Task succeeded: Blastn
[2023-06-04 21:50:24,653] [INFO] Selected 34 target genomes.
[2023-06-04 21:50:24,654] [INFO] Target genome list was writen to GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/target_genomes.txt
[2023-06-04 21:50:24,692] [INFO] Task started: fastANI
[2023-06-04 21:50:24,693] [INFO] Running command: fastANI --query /var/lib/cwl/stga9e272c8-5226-4336-b9e1-1e0c0dd9f0a6/GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna.gz --refList GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/target_genomes.txt --output GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/fastani_result.tsv --threads 1
[2023-06-04 21:50:44,013] [INFO] Task succeeded: fastANI
[2023-06-04 21:50:44,014] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgfec2fd97-ed22-44f4-834e-59eff3dbc71c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-04 21:50:44,015] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgfec2fd97-ed22-44f4-834e-59eff3dbc71c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-04 21:50:44,017] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-04 21:50:44,017] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-04 21:50:44,017] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-04 21:50:44,021] [INFO] DFAST Taxonomy check result was written to GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/tc_result.tsv
[2023-06-04 21:50:44,021] [INFO] ===== Taxonomy check completed =====
[2023-06-04 21:50:44,022] [INFO] ===== Start completeness check using CheckM =====
[2023-06-04 21:50:44,022] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfec2fd97-ed22-44f4-834e-59eff3dbc71c/dqc_reference/checkm_data
[2023-06-04 21:50:44,026] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-04 21:50:44,060] [INFO] Task started: CheckM
[2023-06-04 21:50:44,060] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/checkm_input GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/checkm_result
[2023-06-04 21:51:28,694] [INFO] Task succeeded: CheckM
[2023-06-04 21:51:28,695] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-04 21:51:28,718] [INFO] ===== Completeness check finished =====
[2023-06-04 21:51:28,719] [INFO] ===== Start GTDB Search =====
[2023-06-04 21:51:28,719] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/markers.fasta)
[2023-06-04 21:51:28,719] [INFO] Task started: Blastn
[2023-06-04 21:51:28,720] [INFO] Running command: blastn -query GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/markers.fasta -db /var/lib/cwl/stgfec2fd97-ed22-44f4-834e-59eff3dbc71c/dqc_reference/reference_markers_gtdb.fasta -out GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-04 21:51:29,614] [INFO] Task succeeded: Blastn
[2023-06-04 21:51:29,619] [INFO] Selected 15 target genomes.
[2023-06-04 21:51:29,620] [INFO] Target genome list was writen to GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/target_genomes_gtdb.txt
[2023-06-04 21:51:29,628] [INFO] Task started: fastANI
[2023-06-04 21:51:29,629] [INFO] Running command: fastANI --query /var/lib/cwl/stga9e272c8-5226-4336-b9e1-1e0c0dd9f0a6/GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna.gz --refList GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/target_genomes_gtdb.txt --output GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-04 21:51:36,624] [INFO] Task succeeded: fastANI
[2023-06-04 21:51:36,642] [INFO] Found 14 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-04 21:51:36,642] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002354005.1	s__Cryptobacteroides sp002354005	96.4286	512	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	96.47	96.01	0.76	0.69	14	conclusive
GCA_900317845.1	s__Cryptobacteroides sp900317845	94.9846	649	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	97.57	95.18	0.88	0.74	22	-
GCA_002371115.1	s__Cryptobacteroides sp002371115	87.0	435	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	98.18	97.48	0.78	0.75	4	-
GCA_902788095.1	s__Cryptobacteroides sp902788095	85.3962	456	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	97.63	97.63	0.79	0.79	2	-
GCA_902794005.1	s__Cryptobacteroides sp902794005	81.5437	360	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	98.50	98.20	0.83	0.80	3	-
GCA_017447815.1	s__Cryptobacteroides sp017447815	81.5193	390	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900318445.1	s__Cryptobacteroides sp900318445	81.4989	366	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	96.95	95.41	0.86	0.76	6	-
GCA_902784435.1	s__Cryptobacteroides sp902784435	81.4579	409	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902794615.1	s__Cryptobacteroides sp902794615	81.4403	319	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	97.63	96.28	0.79	0.72	4	-
GCA_017480345.1	s__Cryptobacteroides sp017480345	81.4301	339	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	98.06	98.06	0.83	0.83	2	-
GCA_902774155.1	s__Cryptobacteroides sp902774155	81.3698	388	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	97.49	96.60	0.84	0.80	4	-
GCA_905234795.1	s__Cryptobacteroides sp905234795	80.804	401	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900321655.1	s__Cryptobacteroides sp900321655	80.3715	333	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	98.46	96.30	0.88	0.86	12	-
GCA_900319405.1	s__Cryptobacteroides sp900319405	80.3095	316	807	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__UBA932;g__Cryptobacteroides	95.0	100.00	100.00	0.99	0.99	2	-
--------------------------------------------------------------------------------
[2023-06-04 21:51:36,644] [INFO] GTDB search result was written to GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/result_gtdb.tsv
[2023-06-04 21:51:36,645] [INFO] ===== GTDB Search completed =====
[2023-06-04 21:51:36,648] [INFO] DFAST_QC result json was written to GCA_902781425.1_Rumen_uncultured_genome_RUG12672_genomic.fna/dqc_result.json
[2023-06-04 21:51:36,648] [INFO] DFAST_QC completed!
[2023-06-04 21:51:36,648] [INFO] Total running time: 0h1m30s