[2023-06-30 23:03:02,459] [INFO] DFAST_QC pipeline started.
[2023-06-30 23:03:02,465] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 23:03:02,465] [INFO] DQC Reference Directory: /var/lib/cwl/stg376aee5a-9c41-40e2-a64b-192a3f261dfe/dqc_reference
[2023-06-30 23:03:04,379] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 23:03:04,381] [INFO] Task started: Prodigal
[2023-06-30 23:03:04,381] [INFO] Running command: gunzip -c /var/lib/cwl/stgdc2a05f6-8762-4648-9643-6dda16fb2141/GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna.gz | prodigal -d GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/cds.fna -a GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 23:03:26,064] [INFO] Task succeeded: Prodigal
[2023-06-30 23:03:26,064] [INFO] Task started: HMMsearch
[2023-06-30 23:03:26,065] [INFO] Running command: hmmsearch --tblout GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg376aee5a-9c41-40e2-a64b-192a3f261dfe/dqc_reference/reference_markers.hmm GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/protein.faa > /dev/null
[2023-06-30 23:03:26,314] [INFO] Task succeeded: HMMsearch
[2023-06-30 23:03:26,315] [INFO] Found 6/6 markers.
[2023-06-30 23:03:26,346] [INFO] Query marker FASTA was written to GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/markers.fasta
[2023-06-30 23:03:26,346] [INFO] Task started: Blastn
[2023-06-30 23:03:26,347] [INFO] Running command: blastn -query GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/markers.fasta -db /var/lib/cwl/stg376aee5a-9c41-40e2-a64b-192a3f261dfe/dqc_reference/reference_markers.fasta -out GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 23:03:26,935] [INFO] Task succeeded: Blastn
[2023-06-30 23:03:26,941] [INFO] Selected 20 target genomes.
[2023-06-30 23:03:26,941] [INFO] Target genome list was writen to GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/target_genomes.txt
[2023-06-30 23:03:26,945] [INFO] Task started: fastANI
[2023-06-30 23:03:26,945] [INFO] Running command: fastANI --query /var/lib/cwl/stgdc2a05f6-8762-4648-9643-6dda16fb2141/GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna.gz --refList GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/target_genomes.txt --output GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 23:03:40,250] [INFO] Task succeeded: fastANI
[2023-06-30 23:03:40,251] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg376aee5a-9c41-40e2-a64b-192a3f261dfe/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 23:03:40,252] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg376aee5a-9c41-40e2-a64b-192a3f261dfe/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 23:03:40,254] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 23:03:40,254] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-30 23:03:40,254] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-30 23:03:40,256] [INFO] DFAST Taxonomy check result was written to GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/tc_result.tsv
[2023-06-30 23:03:40,257] [INFO] ===== Taxonomy check completed =====
[2023-06-30 23:03:40,258] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 23:03:40,258] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg376aee5a-9c41-40e2-a64b-192a3f261dfe/dqc_reference/checkm_data
[2023-06-30 23:03:40,262] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 23:03:40,302] [INFO] Task started: CheckM
[2023-06-30 23:03:40,302] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/checkm_input GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/checkm_result
[2023-06-30 23:04:42,255] [INFO] Task succeeded: CheckM
[2023-06-30 23:04:42,257] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 0.46%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-30 23:04:42,280] [INFO] ===== Completeness check finished =====
[2023-06-30 23:04:42,280] [INFO] ===== Start GTDB Search =====
[2023-06-30 23:04:42,281] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/markers.fasta)
[2023-06-30 23:04:42,281] [INFO] Task started: Blastn
[2023-06-30 23:04:42,281] [INFO] Running command: blastn -query GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/markers.fasta -db /var/lib/cwl/stg376aee5a-9c41-40e2-a64b-192a3f261dfe/dqc_reference/reference_markers_gtdb.fasta -out GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 23:04:43,064] [INFO] Task succeeded: Blastn
[2023-06-30 23:04:43,069] [INFO] Selected 31 target genomes.
[2023-06-30 23:04:43,070] [INFO] Target genome list was writen to GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 23:04:43,107] [INFO] Task started: fastANI
[2023-06-30 23:04:43,107] [INFO] Running command: fastANI --query /var/lib/cwl/stgdc2a05f6-8762-4648-9643-6dda16fb2141/GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna.gz --refList GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/target_genomes_gtdb.txt --output GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 23:05:06,939] [INFO] Task succeeded: fastANI
[2023-06-30 23:05:06,945] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-30 23:05:06,945] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_903913865.1	s__UBA693 sp903913865	98.7024	550	586	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Pedosphaerales;f__UBA8199;g__UBA693	95.0	98.52	98.50	0.89	0.88	3	conclusive
--------------------------------------------------------------------------------
[2023-06-30 23:05:06,948] [INFO] GTDB search result was written to GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/result_gtdb.tsv
[2023-06-30 23:05:06,948] [INFO] ===== GTDB Search completed =====
[2023-06-30 23:05:06,952] [INFO] DFAST_QC result json was written to GCA_903822365.1_freshwater_MAG_---_LJ_bin-0026_genomic.fna/dqc_result.json
[2023-06-30 23:05:06,952] [INFO] DFAST_QC completed!
[2023-06-30 23:05:06,952] [INFO] Total running time: 0h2m4s