[2023-06-30 06:10:44,238] [INFO] DFAST_QC pipeline started.
[2023-06-30 06:10:44,240] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 06:10:44,240] [INFO] DQC Reference Directory: /var/lib/cwl/stgb3b75e1c-cf99-42e9-ae54-4c08acb81d7c/dqc_reference
[2023-06-30 06:10:45,593] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 06:10:45,601] [INFO] Task started: Prodigal
[2023-06-30 06:10:45,602] [INFO] Running command: gunzip -c /var/lib/cwl/stg9d7a4444-fde6-4654-8ca6-bb2d293f6276/GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna.gz | prodigal -d GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/cds.fna -a GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 06:10:47,827] [INFO] Task succeeded: Prodigal
[2023-06-30 06:10:47,827] [INFO] Task started: HMMsearch
[2023-06-30 06:10:47,828] [INFO] Running command: hmmsearch --tblout GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb3b75e1c-cf99-42e9-ae54-4c08acb81d7c/dqc_reference/reference_markers.hmm GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/protein.faa > /dev/null
[2023-06-30 06:10:47,992] [INFO] Task succeeded: HMMsearch
[2023-06-30 06:10:47,993] [INFO] Found 6/6 markers.
[2023-06-30 06:10:48,005] [INFO] Query marker FASTA was written to GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/markers.fasta
[2023-06-30 06:10:48,005] [INFO] Task started: Blastn
[2023-06-30 06:10:48,005] [INFO] Running command: blastn -query GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/markers.fasta -db /var/lib/cwl/stgb3b75e1c-cf99-42e9-ae54-4c08acb81d7c/dqc_reference/reference_markers.fasta -out GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 06:10:48,592] [INFO] Task succeeded: Blastn
[2023-06-30 06:10:48,596] [INFO] Selected 15 target genomes.
[2023-06-30 06:10:48,596] [INFO] Target genome list was writen to GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/target_genomes.txt
[2023-06-30 06:10:48,598] [INFO] Task started: fastANI
[2023-06-30 06:10:48,598] [INFO] Running command: fastANI --query /var/lib/cwl/stg9d7a4444-fde6-4654-8ca6-bb2d293f6276/GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna.gz --refList GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/target_genomes.txt --output GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 06:10:56,229] [INFO] Task succeeded: fastANI
[2023-06-30 06:10:56,230] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb3b75e1c-cf99-42e9-ae54-4c08acb81d7c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 06:10:56,230] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb3b75e1c-cf99-42e9-ae54-4c08acb81d7c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 06:10:56,235] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 06:10:56,235] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-30 06:10:56,235] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-30 06:10:56,238] [INFO] DFAST Taxonomy check result was written to GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/tc_result.tsv
[2023-06-30 06:10:56,239] [INFO] ===== Taxonomy check completed =====
[2023-06-30 06:10:56,239] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 06:10:56,240] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb3b75e1c-cf99-42e9-ae54-4c08acb81d7c/dqc_reference/checkm_data
[2023-06-30 06:10:56,244] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 06:10:56,257] [INFO] Task started: CheckM
[2023-06-30 06:10:56,257] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/checkm_input GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/checkm_result
[2023-06-30 06:11:11,825] [INFO] Task succeeded: CheckM
[2023-06-30 06:11:11,828] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 77.40%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-30 06:11:11,861] [INFO] ===== Completeness check finished =====
[2023-06-30 06:11:11,862] [INFO] ===== Start GTDB Search =====
[2023-06-30 06:11:11,862] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/markers.fasta)
[2023-06-30 06:11:11,862] [INFO] Task started: Blastn
[2023-06-30 06:11:11,862] [INFO] Running command: blastn -query GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/markers.fasta -db /var/lib/cwl/stgb3b75e1c-cf99-42e9-ae54-4c08acb81d7c/dqc_reference/reference_markers_gtdb.fasta -out GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 06:11:12,648] [INFO] Task succeeded: Blastn
[2023-06-30 06:11:12,652] [INFO] Selected 14 target genomes.
[2023-06-30 06:11:12,653] [INFO] Target genome list was writen to GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 06:11:12,689] [INFO] Task started: fastANI
[2023-06-30 06:11:12,689] [INFO] Running command: fastANI --query /var/lib/cwl/stg9d7a4444-fde6-4654-8ca6-bb2d293f6276/GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna.gz --refList GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/target_genomes_gtdb.txt --output GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 06:11:14,785] [INFO] Task succeeded: fastANI
[2023-06-30 06:11:14,796] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-30 06:11:14,796] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_903834855.1	s__UBA11704 sp903834855	100.0	182	187	d__Bacteria;p__Patescibacteria;c__Paceibacteria;o__UBA9983_A;f__UBA5272;g__UBA11704	95.0	99.88	99.88	0.85	0.85	2	conclusive
GCA_003518045.1	s__UBA11704 sp003518045	83.9832	146	187	d__Bacteria;p__Patescibacteria;c__Paceibacteria;o__UBA9983_A;f__UBA5272;g__UBA11704	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-30 06:11:14,799] [INFO] GTDB search result was written to GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/result_gtdb.tsv
[2023-06-30 06:11:14,799] [INFO] ===== GTDB Search completed =====
[2023-06-30 06:11:14,802] [INFO] DFAST_QC result json was written to GCA_903834855.1_freshwater_MAG_---_Ja_bin-4026_genomic.fna/dqc_result.json
[2023-06-30 06:11:14,803] [INFO] DFAST_QC completed!
[2023-06-30 06:11:14,803] [INFO] Total running time: 0h0m31s