[2023-06-30 19:52:47,824] [INFO] DFAST_QC pipeline started.
[2023-06-30 19:52:47,827] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 19:52:47,827] [INFO] DQC Reference Directory: /var/lib/cwl/stgb1a20016-28d2-4b15-8877-15bc8239891e/dqc_reference
[2023-06-30 19:52:49,234] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 19:52:49,235] [INFO] Task started: Prodigal
[2023-06-30 19:52:49,235] [INFO] Running command: gunzip -c /var/lib/cwl/stgceff87d4-aca2-45e5-949d-b30a7e1d0e3b/GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna.gz | prodigal -d GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/cds.fna -a GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 19:52:55,787] [INFO] Task succeeded: Prodigal
[2023-06-30 19:52:55,787] [INFO] Task started: HMMsearch
[2023-06-30 19:52:55,787] [INFO] Running command: hmmsearch --tblout GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb1a20016-28d2-4b15-8877-15bc8239891e/dqc_reference/reference_markers.hmm GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/protein.faa > /dev/null
[2023-06-30 19:52:55,996] [INFO] Task succeeded: HMMsearch
[2023-06-30 19:52:55,997] [WARNING] Found 4/6 markers. [/var/lib/cwl/stgceff87d4-aca2-45e5-949d-b30a7e1d0e3b/GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna.gz]
[2023-06-30 19:52:56,016] [INFO] Query marker FASTA was written to GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/markers.fasta
[2023-06-30 19:52:56,017] [INFO] Task started: Blastn
[2023-06-30 19:52:56,017] [INFO] Running command: blastn -query GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/markers.fasta -db /var/lib/cwl/stgb1a20016-28d2-4b15-8877-15bc8239891e/dqc_reference/reference_markers.fasta -out GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 19:52:56,608] [INFO] Task succeeded: Blastn
[2023-06-30 19:52:56,614] [INFO] Selected 15 target genomes.
[2023-06-30 19:52:56,615] [INFO] Target genome list was writen to GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/target_genomes.txt
[2023-06-30 19:52:56,620] [INFO] Task started: fastANI
[2023-06-30 19:52:56,620] [INFO] Running command: fastANI --query /var/lib/cwl/stgceff87d4-aca2-45e5-949d-b30a7e1d0e3b/GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna.gz --refList GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/target_genomes.txt --output GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 19:53:04,570] [INFO] Task succeeded: fastANI
[2023-06-30 19:53:04,570] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb1a20016-28d2-4b15-8877-15bc8239891e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 19:53:04,571] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb1a20016-28d2-4b15-8877-15bc8239891e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 19:53:04,572] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 19:53:04,572] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-30 19:53:04,572] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-30 19:53:04,574] [INFO] DFAST Taxonomy check result was written to GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/tc_result.tsv
[2023-06-30 19:53:04,575] [INFO] ===== Taxonomy check completed =====
[2023-06-30 19:53:04,575] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 19:53:04,575] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb1a20016-28d2-4b15-8877-15bc8239891e/dqc_reference/checkm_data
[2023-06-30 19:53:04,578] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 19:53:04,595] [INFO] Task started: CheckM
[2023-06-30 19:53:04,595] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/checkm_input GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/checkm_result
[2023-06-30 19:53:29,923] [INFO] Task succeeded: CheckM
[2023-06-30 19:53:29,925] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.94%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-30 19:53:29,950] [INFO] ===== Completeness check finished =====
[2023-06-30 19:53:29,951] [INFO] ===== Start GTDB Search =====
[2023-06-30 19:53:29,951] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/markers.fasta)
[2023-06-30 19:53:29,951] [INFO] Task started: Blastn
[2023-06-30 19:53:29,952] [INFO] Running command: blastn -query GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/markers.fasta -db /var/lib/cwl/stgb1a20016-28d2-4b15-8877-15bc8239891e/dqc_reference/reference_markers_gtdb.fasta -out GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 19:53:30,681] [INFO] Task succeeded: Blastn
[2023-06-30 19:53:30,685] [INFO] Selected 13 target genomes.
[2023-06-30 19:53:30,685] [INFO] Target genome list was writen to GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 19:53:30,728] [INFO] Task started: fastANI
[2023-06-30 19:53:30,728] [INFO] Running command: fastANI --query /var/lib/cwl/stgceff87d4-aca2-45e5-949d-b30a7e1d0e3b/GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna.gz --refList GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/target_genomes_gtdb.txt --output GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 19:53:34,221] [INFO] Task succeeded: fastANI
[2023-06-30 19:53:34,226] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-30 19:53:34,226] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_903847095.1	s__CAINCQ01 sp903847095	99.8945	404	422	d__Bacteria;p__Patescibacteria;c__Paceibacteria;o__Paceibacterales;f__UBA5738;g__CAINCQ01	95.0	99.92	99.87	0.96	0.94	10	conclusive
--------------------------------------------------------------------------------
[2023-06-30 19:53:34,229] [INFO] GTDB search result was written to GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/result_gtdb.tsv
[2023-06-30 19:53:34,229] [INFO] ===== GTDB Search completed =====
[2023-06-30 19:53:34,247] [INFO] DFAST_QC result json was written to GCA_903862575.1_freshwater_MAG_---_AlinenSedimentsCore2_bin-1270_genomic.fna/dqc_result.json
[2023-06-30 19:53:34,248] [INFO] DFAST_QC completed!
[2023-06-30 19:53:34,248] [INFO] Total running time: 0h0m46s