[2023-06-30 16:08:38,895] [INFO] DFAST_QC pipeline started.
[2023-06-30 16:08:38,897] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 16:08:38,897] [INFO] DQC Reference Directory: /var/lib/cwl/stg76efd488-bc37-4d19-8dd8-18238fdc3e6a/dqc_reference
[2023-06-30 16:08:40,163] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 16:08:40,164] [INFO] Task started: Prodigal
[2023-06-30 16:08:40,164] [INFO] Running command: gunzip -c /var/lib/cwl/stgdbc05167-b630-4404-8436-10e52070407e/GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna.gz | prodigal -d GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/cds.fna -a GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 16:08:41,882] [INFO] Task succeeded: Prodigal
[2023-06-30 16:08:41,883] [INFO] Task started: HMMsearch
[2023-06-30 16:08:41,883] [INFO] Running command: hmmsearch --tblout GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg76efd488-bc37-4d19-8dd8-18238fdc3e6a/dqc_reference/reference_markers.hmm GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/protein.faa > /dev/null
[2023-06-30 16:08:42,028] [INFO] Task succeeded: HMMsearch
[2023-06-30 16:08:42,030] [WARNING] Found 4/6 markers. [/var/lib/cwl/stgdbc05167-b630-4404-8436-10e52070407e/GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna.gz]
[2023-06-30 16:08:42,042] [INFO] Query marker FASTA was written to GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/markers.fasta
[2023-06-30 16:08:42,043] [INFO] Task started: Blastn
[2023-06-30 16:08:42,043] [INFO] Running command: blastn -query GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/markers.fasta -db /var/lib/cwl/stg76efd488-bc37-4d19-8dd8-18238fdc3e6a/dqc_reference/reference_markers.fasta -out GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 16:08:42,594] [INFO] Task succeeded: Blastn
[2023-06-30 16:08:42,597] [INFO] Selected 15 target genomes.
[2023-06-30 16:08:42,598] [INFO] Target genome list was writen to GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/target_genomes.txt
[2023-06-30 16:08:42,599] [INFO] Task started: fastANI
[2023-06-30 16:08:42,599] [INFO] Running command: fastANI --query /var/lib/cwl/stgdbc05167-b630-4404-8436-10e52070407e/GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna.gz --refList GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/target_genomes.txt --output GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 16:08:51,899] [INFO] Task succeeded: fastANI
[2023-06-30 16:08:51,899] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg76efd488-bc37-4d19-8dd8-18238fdc3e6a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 16:08:51,899] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg76efd488-bc37-4d19-8dd8-18238fdc3e6a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 16:08:51,901] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 16:08:51,902] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-30 16:08:51,902] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-30 16:08:51,904] [INFO] DFAST Taxonomy check result was written to GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/tc_result.tsv
[2023-06-30 16:08:51,904] [INFO] ===== Taxonomy check completed =====
[2023-06-30 16:08:51,904] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 16:08:51,904] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg76efd488-bc37-4d19-8dd8-18238fdc3e6a/dqc_reference/checkm_data
[2023-06-30 16:08:51,907] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 16:08:51,919] [INFO] Task started: CheckM
[2023-06-30 16:08:51,920] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/checkm_input GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/checkm_result
[2023-06-30 16:09:04,779] [INFO] Task succeeded: CheckM
[2023-06-30 16:09:04,780] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 87.50%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-30 16:09:04,797] [INFO] ===== Completeness check finished =====
[2023-06-30 16:09:04,797] [INFO] ===== Start GTDB Search =====
[2023-06-30 16:09:04,797] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/markers.fasta)
[2023-06-30 16:09:04,798] [INFO] Task started: Blastn
[2023-06-30 16:09:04,798] [INFO] Running command: blastn -query GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/markers.fasta -db /var/lib/cwl/stg76efd488-bc37-4d19-8dd8-18238fdc3e6a/dqc_reference/reference_markers_gtdb.fasta -out GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 16:09:05,553] [INFO] Task succeeded: Blastn
[2023-06-30 16:09:05,556] [INFO] Selected 13 target genomes.
[2023-06-30 16:09:05,557] [INFO] Target genome list was writen to GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 16:09:05,562] [INFO] Task started: fastANI
[2023-06-30 16:09:05,563] [INFO] Running command: fastANI --query /var/lib/cwl/stgdbc05167-b630-4404-8436-10e52070407e/GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna.gz --refList GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/target_genomes_gtdb.txt --output GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 16:09:12,210] [INFO] Task succeeded: fastANI
[2023-06-30 16:09:12,212] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-30 16:09:12,212] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-06-30 16:09:12,214] [INFO] GTDB search result was written to GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/result_gtdb.tsv
[2023-06-30 16:09:12,215] [INFO] ===== GTDB Search completed =====
[2023-06-30 16:09:12,217] [INFO] DFAST_QC result json was written to GCA_903866265.1_freshwater_MAG_---_F14_bin-0088_genomic.fna/dqc_result.json
[2023-06-30 16:09:12,217] [INFO] DFAST_QC completed!
[2023-06-30 16:09:12,217] [INFO] Total running time: 0h0m33s