[2023-06-30 19:16:05,275] [INFO] DFAST_QC pipeline started.
[2023-06-30 19:16:05,277] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 19:16:05,277] [INFO] DQC Reference Directory: /var/lib/cwl/stg20e1c64f-1bbd-4109-b86a-0d624691b23d/dqc_reference
[2023-06-30 19:16:06,546] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 19:16:06,547] [INFO] Task started: Prodigal
[2023-06-30 19:16:06,547] [INFO] Running command: gunzip -c /var/lib/cwl/stged6259c4-88f7-4542-bd00-5feecb0350b7/GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna.gz | prodigal -d GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/cds.fna -a GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 19:16:10,408] [INFO] Task succeeded: Prodigal
[2023-06-30 19:16:10,408] [INFO] Task started: HMMsearch
[2023-06-30 19:16:10,408] [INFO] Running command: hmmsearch --tblout GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg20e1c64f-1bbd-4109-b86a-0d624691b23d/dqc_reference/reference_markers.hmm GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/protein.faa > /dev/null
[2023-06-30 19:16:10,660] [INFO] Task succeeded: HMMsearch
[2023-06-30 19:16:10,661] [WARNING] Found 4/6 markers. [/var/lib/cwl/stged6259c4-88f7-4542-bd00-5feecb0350b7/GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna.gz]
[2023-06-30 19:16:10,673] [INFO] Query marker FASTA was written to GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/markers.fasta
[2023-06-30 19:16:10,673] [INFO] Task started: Blastn
[2023-06-30 19:16:10,674] [INFO] Running command: blastn -query GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/markers.fasta -db /var/lib/cwl/stg20e1c64f-1bbd-4109-b86a-0d624691b23d/dqc_reference/reference_markers.fasta -out GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 19:16:11,282] [INFO] Task succeeded: Blastn
[2023-06-30 19:16:11,292] [INFO] Selected 10 target genomes.
[2023-06-30 19:16:11,292] [INFO] Target genome list was writen to GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/target_genomes.txt
[2023-06-30 19:16:11,294] [INFO] Task started: fastANI
[2023-06-30 19:16:11,294] [INFO] Running command: fastANI --query /var/lib/cwl/stged6259c4-88f7-4542-bd00-5feecb0350b7/GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna.gz --refList GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/target_genomes.txt --output GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 19:16:17,933] [INFO] Task succeeded: fastANI
[2023-06-30 19:16:17,934] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg20e1c64f-1bbd-4109-b86a-0d624691b23d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 19:16:17,934] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg20e1c64f-1bbd-4109-b86a-0d624691b23d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 19:16:17,935] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 19:16:17,936] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-30 19:16:17,936] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-30 19:16:17,938] [INFO] DFAST Taxonomy check result was written to GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/tc_result.tsv
[2023-06-30 19:16:17,938] [INFO] ===== Taxonomy check completed =====
[2023-06-30 19:16:17,938] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 19:16:17,939] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg20e1c64f-1bbd-4109-b86a-0d624691b23d/dqc_reference/checkm_data
[2023-06-30 19:16:17,941] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 19:16:17,955] [INFO] Task started: CheckM
[2023-06-30 19:16:17,955] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/checkm_input GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/checkm_result
[2023-06-30 19:16:36,384] [INFO] Task succeeded: CheckM
[2023-06-30 19:16:36,385] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 74.42%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-30 19:16:36,401] [INFO] ===== Completeness check finished =====
[2023-06-30 19:16:36,401] [INFO] ===== Start GTDB Search =====
[2023-06-30 19:16:36,402] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/markers.fasta)
[2023-06-30 19:16:36,402] [INFO] Task started: Blastn
[2023-06-30 19:16:36,402] [INFO] Running command: blastn -query GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/markers.fasta -db /var/lib/cwl/stg20e1c64f-1bbd-4109-b86a-0d624691b23d/dqc_reference/reference_markers_gtdb.fasta -out GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 19:16:37,177] [INFO] Task succeeded: Blastn
[2023-06-30 19:16:37,181] [INFO] Selected 12 target genomes.
[2023-06-30 19:16:37,182] [INFO] Target genome list was writen to GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 19:16:37,188] [INFO] Task started: fastANI
[2023-06-30 19:16:37,189] [INFO] Running command: fastANI --query /var/lib/cwl/stged6259c4-88f7-4542-bd00-5feecb0350b7/GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna.gz --refList GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/target_genomes_gtdb.txt --output GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 19:16:39,035] [INFO] Task succeeded: fastANI
[2023-06-30 19:16:39,039] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-30 19:16:39,040] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_903854215.1	s__UBA10103 sp903854215	99.7135	112	116	d__Bacteria;p__Patescibacteria;c__Paceibacteria;o__UBA9983_A;f__UBA2100;g__UBA10103	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_003164215.1	s__UBA10103 sp003164215	79.592	54	116	d__Bacteria;p__Patescibacteria;c__Paceibacteria;o__UBA9983_A;f__UBA2100;g__UBA10103	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-30 19:16:39,042] [INFO] GTDB search result was written to GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/result_gtdb.tsv
[2023-06-30 19:16:39,042] [INFO] ===== GTDB Search completed =====
[2023-06-30 19:16:39,045] [INFO] DFAST_QC result json was written to GCA_903869065.1_freshwater_MAG_---_C4_bin-840_genomic.fna/dqc_result.json
[2023-06-30 19:16:39,045] [INFO] DFAST_QC completed!
[2023-06-30 19:16:39,045] [INFO] Total running time: 0h0m34s