[2023-06-30 07:54:55,772] [INFO] DFAST_QC pipeline started.
[2023-06-30 07:54:55,777] [INFO] DFAST_QC version: 0.5.7
[2023-06-30 07:54:55,777] [INFO] DQC Reference Directory: /var/lib/cwl/stg3b5756fe-f69e-43ff-a404-fa6eb63b1e72/dqc_reference
[2023-06-30 07:54:58,446] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-30 07:54:58,447] [INFO] Task started: Prodigal
[2023-06-30 07:54:58,448] [INFO] Running command: gunzip -c /var/lib/cwl/stg32cc977d-54f9-489f-933c-4f7417f6981c/GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna.gz | prodigal -d GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/cds.fna -a GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-30 07:55:05,763] [INFO] Task succeeded: Prodigal
[2023-06-30 07:55:05,764] [INFO] Task started: HMMsearch
[2023-06-30 07:55:05,764] [INFO] Running command: hmmsearch --tblout GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3b5756fe-f69e-43ff-a404-fa6eb63b1e72/dqc_reference/reference_markers.hmm GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/protein.faa > /dev/null
[2023-06-30 07:55:06,047] [INFO] Task succeeded: HMMsearch
[2023-06-30 07:55:06,049] [INFO] Found 6/6 markers.
[2023-06-30 07:55:06,082] [INFO] Query marker FASTA was written to GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/markers.fasta
[2023-06-30 07:55:06,082] [INFO] Task started: Blastn
[2023-06-30 07:55:06,082] [INFO] Running command: blastn -query GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/markers.fasta -db /var/lib/cwl/stg3b5756fe-f69e-43ff-a404-fa6eb63b1e72/dqc_reference/reference_markers.fasta -out GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 07:55:06,674] [INFO] Task succeeded: Blastn
[2023-06-30 07:55:06,679] [INFO] Selected 16 target genomes.
[2023-06-30 07:55:06,679] [INFO] Target genome list was writen to GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/target_genomes.txt
[2023-06-30 07:55:06,680] [INFO] Task started: fastANI
[2023-06-30 07:55:06,680] [INFO] Running command: fastANI --query /var/lib/cwl/stg32cc977d-54f9-489f-933c-4f7417f6981c/GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna.gz --refList GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/target_genomes.txt --output GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/fastani_result.tsv --threads 1
[2023-06-30 07:55:14,266] [INFO] Task succeeded: fastANI
[2023-06-30 07:55:14,267] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3b5756fe-f69e-43ff-a404-fa6eb63b1e72/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-30 07:55:14,267] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3b5756fe-f69e-43ff-a404-fa6eb63b1e72/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-30 07:55:14,278] [INFO] Found 4 fastANI hits (0 hits with ANI > threshold)
[2023-06-30 07:55:14,278] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-06-30 07:55:14,278] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Pelodictyon phaeoclathratiforme	strain=BU-1	GCA_000020645.1	34090	34090	type	True	79.1286	339	924	95	below_threshold
Chlorobium ferrooxidans	strain=DSM 13031	GCA_000168715.1	84205	84205	type	True	77.9804	250	924	95	below_threshold
Chlorobium phaeobacteroides	strain=DSM 266	GCA_000015125.1	1096	1096	type	True	77.5252	160	924	95	below_threshold
Chlorobium limicola	strain=DSM 245	GCA_000020465.1	1092	1092	type	True	76.8427	131	924	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-30 07:55:14,281] [INFO] DFAST Taxonomy check result was written to GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/tc_result.tsv
[2023-06-30 07:55:14,281] [INFO] ===== Taxonomy check completed =====
[2023-06-30 07:55:14,281] [INFO] ===== Start completeness check using CheckM =====
[2023-06-30 07:55:14,282] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3b5756fe-f69e-43ff-a404-fa6eb63b1e72/dqc_reference/checkm_data
[2023-06-30 07:55:14,283] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-30 07:55:14,320] [INFO] Task started: CheckM
[2023-06-30 07:55:14,321] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/checkm_input GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/checkm_result
[2023-06-30 07:55:43,251] [INFO] Task succeeded: CheckM
[2023-06-30 07:55:43,252] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.38%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-06-30 07:55:43,275] [INFO] ===== Completeness check finished =====
[2023-06-30 07:55:43,275] [INFO] ===== Start GTDB Search =====
[2023-06-30 07:55:43,276] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/markers.fasta)
[2023-06-30 07:55:43,276] [INFO] Task started: Blastn
[2023-06-30 07:55:43,276] [INFO] Running command: blastn -query GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/markers.fasta -db /var/lib/cwl/stg3b5756fe-f69e-43ff-a404-fa6eb63b1e72/dqc_reference/reference_markers_gtdb.fasta -out GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-30 07:55:44,089] [INFO] Task succeeded: Blastn
[2023-06-30 07:55:44,094] [INFO] Selected 9 target genomes.
[2023-06-30 07:55:44,094] [INFO] Target genome list was writen to GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/target_genomes_gtdb.txt
[2023-06-30 07:55:44,096] [INFO] Task started: fastANI
[2023-06-30 07:55:44,096] [INFO] Running command: fastANI --query /var/lib/cwl/stg32cc977d-54f9-489f-933c-4f7417f6981c/GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna.gz --refList GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/target_genomes_gtdb.txt --output GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-30 07:55:49,373] [INFO] Task succeeded: fastANI
[2023-06-30 07:55:49,384] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-30 07:55:49,384] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_903869365.1	s__Chlorobium sp903869365	99.8387	843	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	99.79	99.50	0.93	0.82	33	conclusive
GCA_012972825.1	s__Chlorobium sp012972825	85.9073	628	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_903940125.1	s__Chlorobium sp903940125	85.2386	609	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	98.50	97.06	0.88	0.72	14	-
GCA_903851385.1	s__Chlorobium sp903851385	82.4586	550	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	99.86	99.82	0.97	0.97	3	-
GCA_012972835.1	s__Chlorobium sp012972835	82.4417	593	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_005843825.1	s__Chlorobium sp005843825	82.3714	522	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	98.19	97.55	0.89	0.82	50	-
GCA_903920215.1	s__Chlorobium sp903920215	82.2425	537	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_903904495.1	s__Chlorobium sp903904495	82.0111	362	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	98.87	98.87	0.74	0.74	2	-
GCA_903895195.1	s__Chlorobium sp903895195	81.8343	525	924	d__Bacteria;p__Bacteroidota;c__Chlorobia;o__Chlorobiales;f__Chlorobiaceae;g__Chlorobium	95.0	99.16	98.65	0.92	0.86	65	-
--------------------------------------------------------------------------------
[2023-06-30 07:55:49,417] [INFO] GTDB search result was written to GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/result_gtdb.tsv
[2023-06-30 07:55:49,418] [INFO] ===== GTDB Search completed =====
[2023-06-30 07:55:49,422] [INFO] DFAST_QC result json was written to GCA_903893455.1_freshwater_MAG_---_AM-2014-D4_bin-427_genomic.fna/dqc_result.json
[2023-06-30 07:55:49,422] [INFO] DFAST_QC completed!
[2023-06-30 07:55:49,422] [INFO] Total running time: 0h0m54s