[2023-07-01 07:47:18,433] [INFO] DFAST_QC pipeline started.
[2023-07-01 07:47:18,436] [INFO] DFAST_QC version: 0.5.7
[2023-07-01 07:47:18,436] [INFO] DQC Reference Directory: /var/lib/cwl/stgae66adfc-e41f-462f-afad-897197efe932/dqc_reference
[2023-07-01 07:47:19,645] [INFO] ===== Start taxonomy check using ANI =====
[2023-07-01 07:47:19,646] [INFO] Task started: Prodigal
[2023-07-01 07:47:19,646] [INFO] Running command: gunzip -c /var/lib/cwl/stg872beedd-52f3-44be-b59d-d7798a517dca/GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna.gz | prodigal -d GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/cds.fna -a GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-07-01 07:47:29,417] [INFO] Task succeeded: Prodigal
[2023-07-01 07:47:29,417] [INFO] Task started: HMMsearch
[2023-07-01 07:47:29,417] [INFO] Running command: hmmsearch --tblout GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgae66adfc-e41f-462f-afad-897197efe932/dqc_reference/reference_markers.hmm GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/protein.faa > /dev/null
[2023-07-01 07:47:29,737] [INFO] Task succeeded: HMMsearch
[2023-07-01 07:47:29,739] [INFO] Found 6/6 markers.
[2023-07-01 07:47:29,788] [INFO] Query marker FASTA was written to GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/markers.fasta
[2023-07-01 07:47:29,789] [INFO] Task started: Blastn
[2023-07-01 07:47:29,789] [INFO] Running command: blastn -query GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/markers.fasta -db /var/lib/cwl/stgae66adfc-e41f-462f-afad-897197efe932/dqc_reference/reference_markers.fasta -out GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-07-01 07:47:30,370] [INFO] Task succeeded: Blastn
[2023-07-01 07:47:30,387] [INFO] Selected 20 target genomes.
[2023-07-01 07:47:30,388] [INFO] Target genome list was writen to GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/target_genomes.txt
[2023-07-01 07:47:30,391] [INFO] Task started: fastANI
[2023-07-01 07:47:30,391] [INFO] Running command: fastANI --query /var/lib/cwl/stg872beedd-52f3-44be-b59d-d7798a517dca/GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna.gz --refList GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/target_genomes.txt --output GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/fastani_result.tsv --threads 1
[2023-07-01 07:47:46,386] [INFO] Task succeeded: fastANI
[2023-07-01 07:47:46,386] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgae66adfc-e41f-462f-afad-897197efe932/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-07-01 07:47:46,386] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgae66adfc-e41f-462f-afad-897197efe932/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-07-01 07:47:46,388] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-07-01 07:47:46,388] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-07-01 07:47:46,388] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-07-01 07:47:46,392] [INFO] DFAST Taxonomy check result was written to GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/tc_result.tsv
[2023-07-01 07:47:46,393] [INFO] ===== Taxonomy check completed =====
[2023-07-01 07:47:46,393] [INFO] ===== Start completeness check using CheckM =====
[2023-07-01 07:47:46,393] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgae66adfc-e41f-462f-afad-897197efe932/dqc_reference/checkm_data
[2023-07-01 07:47:46,396] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-07-01 07:47:46,456] [INFO] Task started: CheckM
[2023-07-01 07:47:46,456] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/checkm_input GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/checkm_result
[2023-07-01 07:48:20,716] [INFO] Task succeeded: CheckM
[2023-07-01 07:48:20,717] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 94.18%
Contamintation: 2.08%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-07-01 07:48:20,742] [INFO] ===== Completeness check finished =====
[2023-07-01 07:48:20,742] [INFO] ===== Start GTDB Search =====
[2023-07-01 07:48:20,743] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/markers.fasta)
[2023-07-01 07:48:20,743] [INFO] Task started: Blastn
[2023-07-01 07:48:20,743] [INFO] Running command: blastn -query GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/markers.fasta -db /var/lib/cwl/stgae66adfc-e41f-462f-afad-897197efe932/dqc_reference/reference_markers_gtdb.fasta -out GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-07-01 07:48:21,464] [INFO] Task succeeded: Blastn
[2023-07-01 07:48:21,482] [INFO] Selected 12 target genomes.
[2023-07-01 07:48:21,482] [INFO] Target genome list was writen to GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/target_genomes_gtdb.txt
[2023-07-01 07:48:21,486] [INFO] Task started: fastANI
[2023-07-01 07:48:21,486] [INFO] Running command: fastANI --query /var/lib/cwl/stg872beedd-52f3-44be-b59d-d7798a517dca/GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna.gz --refList GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/target_genomes_gtdb.txt --output GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-07-01 07:48:35,013] [INFO] Task succeeded: fastANI
[2023-07-01 07:48:35,030] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2023-07-01 07:48:35,030] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_903819605.1	s__Pseudanabaena sp903819605	97.1948	947	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	97.61	97.57	0.80	0.79	3	conclusive
GCA_003242085.1	s__Pseudanabaena frigida	84.4473	939	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002251945.1	s__Pseudanabaena sp002251945	81.4069	758	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002914585.1	s__Pseudanabaena sp002914585	81.3181	774	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003967015.1	s__Pseudanabaena sp003967015	81.1989	761	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014696925.1	s__Pseudanabaena sp014696925	81.1814	771	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016584665.1	s__Pseudanabaena sp016584665	80.9337	787	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCF_006937785.1	s__Pseudanabaena sp006937785	80.7795	759	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003249035.1	s__Pseudanabaena sp003249035	80.5461	660	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002325645.1	s__Pseudanabaena sp002325645	80.4274	634	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014696355.1	s__Pseudanabaena sp014696355	80.2639	602	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014696345.1	s__Pseudanabaena sp014696345	78.57	380	1378	d__Bacteria;p__Cyanobacteria;c__Cyanobacteriia;o__Pseudanabaenales;f__Pseudanabaenaceae;g__Pseudanabaena	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-07-01 07:48:35,048] [INFO] GTDB search result was written to GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/result_gtdb.tsv
[2023-07-01 07:48:35,048] [INFO] ===== GTDB Search completed =====
[2023-07-01 07:48:35,089] [INFO] DFAST_QC result json was written to GCA_903913405.1_freshwater_MAG_---_T7_bin-034_genomic.fna/dqc_result.json
[2023-07-01 07:48:35,089] [INFO] DFAST_QC completed!
[2023-07-01 07:48:35,089] [INFO] Total running time: 0h1m17s