[2023-06-17 15:46:52,747] [INFO] DFAST_QC pipeline started.
[2023-06-17 15:46:52,753] [INFO] DFAST_QC version: 0.5.7
[2023-06-17 15:46:52,754] [INFO] DQC Reference Directory: /var/lib/cwl/stgca0f6981-e445-4a68-a0a4-6db0d1c62e49/dqc_reference
[2023-06-17 15:46:55,476] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-17 15:46:55,478] [INFO] Task started: Prodigal
[2023-06-17 15:46:55,480] [INFO] Running command: gunzip -c /var/lib/cwl/stgc5512502-768f-495e-935e-aee172dae405/GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna.gz | prodigal -d GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/cds.fna -a GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-17 15:47:00,780] [INFO] Task succeeded: Prodigal
[2023-06-17 15:47:00,780] [INFO] Task started: HMMsearch
[2023-06-17 15:47:00,781] [INFO] Running command: hmmsearch --tblout GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgca0f6981-e445-4a68-a0a4-6db0d1c62e49/dqc_reference/reference_markers.hmm GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/protein.faa > /dev/null
[2023-06-17 15:47:01,042] [INFO] Task succeeded: HMMsearch
[2023-06-17 15:47:01,043] [WARNING] Found 4/6 markers. [/var/lib/cwl/stgc5512502-768f-495e-935e-aee172dae405/GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna.gz]
[2023-06-17 15:47:01,063] [INFO] Query marker FASTA was written to GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/markers.fasta
[2023-06-17 15:47:01,063] [INFO] Task started: Blastn
[2023-06-17 15:47:01,063] [INFO] Running command: blastn -query GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/markers.fasta -db /var/lib/cwl/stgca0f6981-e445-4a68-a0a4-6db0d1c62e49/dqc_reference/reference_markers.fasta -out GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-17 15:47:01,810] [INFO] Task succeeded: Blastn
[2023-06-17 15:47:01,815] [INFO] Selected 7 target genomes.
[2023-06-17 15:47:01,816] [INFO] Target genome list was writen to GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/target_genomes.txt
[2023-06-17 15:47:01,817] [INFO] Task started: fastANI
[2023-06-17 15:47:01,817] [INFO] Running command: fastANI --query /var/lib/cwl/stgc5512502-768f-495e-935e-aee172dae405/GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna.gz --refList GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/target_genomes.txt --output GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/fastani_result.tsv --threads 1
[2023-06-17 15:47:05,896] [INFO] Task succeeded: fastANI
[2023-06-17 15:47:05,896] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgca0f6981-e445-4a68-a0a4-6db0d1c62e49/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-17 15:47:05,897] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgca0f6981-e445-4a68-a0a4-6db0d1c62e49/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-17 15:47:05,906] [INFO] Found 7 fastANI hits (0 hits with ANI > threshold)
[2023-06-17 15:47:05,907] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-06-17 15:47:05,907] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Faecalibacterium prausnitzii	strain=ATCC 27768	GCA_003324185.1	853	853	suspected-type	True	85.0697	412	539	95	below_threshold
Faecalibacterium duncaniae	strain=JCM 31915	GCA_010509575.1	411483	411483	type	True	83.4279	378	539	95	below_threshold
Faecalibacterium duncaniae	strain=A2-165	GCA_000162015.1	411483	411483	type	True	83.3984	381	539	95	below_threshold
Faecalibacterium hattorii	strain=APC922/41-1	GCA_003287455.1	2935520	2935520	type	True	83.1912	376	539	95	below_threshold
Faecalibacterium gallinarum	strain=JCM 17207	GCA_022180365.1	2903556	2903556	type	True	80.0719	270	539	95	below_threshold
Subdoligranulum variabile	strain=DSM 15176	GCA_025152575.1	214851	214851	type	True	77.7206	119	539	95	below_threshold
Fournierella massiliensis	strain=DSM 100451	GCA_004345265.1	1650663	1650663	type	True	77.447	140	539	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-17 15:47:05,909] [INFO] DFAST Taxonomy check result was written to GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/tc_result.tsv
[2023-06-17 15:47:05,910] [INFO] ===== Taxonomy check completed =====
[2023-06-17 15:47:05,910] [INFO] ===== Start completeness check using CheckM =====
[2023-06-17 15:47:05,910] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgca0f6981-e445-4a68-a0a4-6db0d1c62e49/dqc_reference/checkm_data
[2023-06-17 15:47:05,911] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-17 15:47:05,933] [INFO] Task started: CheckM
[2023-06-17 15:47:05,934] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/checkm_input GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/checkm_result
[2023-06-17 15:47:27,897] [INFO] Task succeeded: CheckM
[2023-06-17 15:47:27,902] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 35.42%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-17 15:47:27,921] [INFO] ===== Completeness check finished =====
[2023-06-17 15:47:27,921] [INFO] ===== Start GTDB Search =====
[2023-06-17 15:47:27,922] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/markers.fasta)
[2023-06-17 15:47:27,922] [INFO] Task started: Blastn
[2023-06-17 15:47:27,922] [INFO] Running command: blastn -query GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/markers.fasta -db /var/lib/cwl/stgca0f6981-e445-4a68-a0a4-6db0d1c62e49/dqc_reference/reference_markers_gtdb.fasta -out GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-17 15:47:29,042] [INFO] Task succeeded: Blastn
[2023-06-17 15:47:29,047] [INFO] Selected 10 target genomes.
[2023-06-17 15:47:29,047] [INFO] Target genome list was writen to GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/target_genomes_gtdb.txt
[2023-06-17 15:47:29,049] [INFO] Task started: fastANI
[2023-06-17 15:47:29,049] [INFO] Running command: fastANI --query /var/lib/cwl/stgc5512502-768f-495e-935e-aee172dae405/GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna.gz --refList GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/target_genomes_gtdb.txt --output GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-17 15:47:34,294] [INFO] Task succeeded: fastANI
[2023-06-17 15:47:34,307] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-17 15:47:34,307] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_900765105.1	s__Faecalibacterium sp900765105	98.2689	441	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0	98.27	98.27	0.82	0.82	2	conclusive
GCF_002549775.1	s__Faecalibacterium prausnitzii_F	87.1313	459	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0	97.99	97.71	0.95	0.94	3	-
GCA_900539945.1	s__Faecalibacterium sp900539945	86.872	409	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0	98.71	98.00	0.92	0.86	4	-
GCF_002549755.1	s__Faecalibacterium prausnitzii_D	86.684	437	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0	97.37	96.64	0.86	0.78	38	-
GCA_900539885.1	s__Faecalibacterium sp900539885	86.5375	423	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0	97.54	97.23	0.89	0.84	6	-
GCF_003324185.1	s__Faecalibacterium prausnitzii	85.0697	412	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0612	96.64	96.12	0.86	0.79	47	-
GCA_003449675.1	s__Faecalibacterium sp003449675	85.0099	303	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0	96.56	96.55	0.80	0.74	3	-
GCA_900772565.1	s__Faecalibacterium sp900772565	84.8933	390	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002550015.1	s__Faecalibacterium prausnitzii_A	84.8914	409	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0612	96.42	95.34	0.88	0.83	7	-
GCA_905215595.1	s__Faecalibacterium sp905215595	84.6255	347	539	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Ruminococcaceae;g__Faecalibacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-17 15:47:34,309] [INFO] GTDB search result was written to GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/result_gtdb.tsv
[2023-06-17 15:47:34,310] [INFO] ===== GTDB Search completed =====
[2023-06-17 15:47:34,327] [INFO] DFAST_QC result json was written to GCA_905206045.1_ERR1430504-mag-bin.13_genomic.fna/dqc_result.json
[2023-06-17 15:47:34,328] [INFO] DFAST_QC completed!
[2023-06-17 15:47:34,328] [INFO] Total running time: 0h0m42s