<?xml version="1.0" ?>
<BioSampleSet><BioSample access="public" publication_date="2021-09-16T00:00:00.000" last_update="2021-09-16T19:23:24.000" submission_date="2021-09-25T10:52:44.978" id="21734168" accession="SAMEA9694995">   <Ids>     <Id db="BioSample" is_primary="1">SAMEA9694995</Id>     <Id db="SRA">ERS7419240</Id>   </Ids>   <Description>     <Title>Metagenome-assembled genome: SRR3580187_bin.31_MetaBAT_v2.12.1_MAG</Title>     <Organism taxonomy_id="154336" taxonomy_name="uncultured Colwellia sp.">       <OrganismName>uncultured Colwellia sp.</OrganismName>     </Organism>     <Comment>       <Paragraph>This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3580187 of study SRP075617.</Paragraph>     </Comment>   </Description>   <Owner>     <Name>EBI</Name>   </Owner>   <Models>     <Model>Generic</Model>   </Models>   <Package display_name="Generic">Generic.1.0</Package>   <Attributes>     <Attribute attribute_name="ENA first public">2021-09-16</Attribute>     <Attribute attribute_name="ENA last update">2021-09-16</Attribute>     <Attribute attribute_name="ENA-CHECKLIST">ERC000047</Attribute>     <Attribute attribute_name="External Id">SAMEA9694995</Attribute>     <Attribute attribute_name="INSDC center alias">EMG</Attribute>     <Attribute attribute_name="INSDC center name">EMG</Attribute>     <Attribute attribute_name="INSDC first public">2021-09-16T16:25:22Z</Attribute>     <Attribute attribute_name="INSDC last update">2021-09-16T16:25:22Z</Attribute>     <Attribute attribute_name="INSDC status">public</Attribute>     <Attribute attribute_name="Submitter Id">SRR3580187_bin.31_MetaBAT_v2.12.1_MAG</Attribute>     <Attribute attribute_name="assembly quality">Many fragments with little to no review of assembly other than reporting of standard assembly statistics</Attribute>     <Attribute attribute_name="assembly software">metaspadesv3.11.1</Attribute>     <Attribute attribute_name="binning parameters">Default</Attribute>     <Attribute attribute_name="binning software">MetaBAT v2.12.1</Attribute>     <Attribute attribute_name="broker name">EMG broker account, EMBL-EBI</Attribute>     <Attribute attribute_name="collection date" harmonized_name="collection_date" display_name="collection date">2013-07-27</Attribute>     <Attribute attribute_name="completeness score">87.3</Attribute>     <Attribute attribute_name="completeness software">CheckM</Attribute>     <Attribute attribute_name="contamination score">4.34</Attribute>     <Attribute attribute_name="environment (biome)" harmonized_name="env_broad_scale" display_name="broad-scale environmental context">aquatic</Attribute>     <Attribute attribute_name="environment (feature)" harmonized_name="env_local_scale" display_name="local-scale environmental context">marine</Attribute>     <Attribute attribute_name="environment (material)" harmonized_name="env_medium" display_name="environmental medium">water</Attribute>     <Attribute attribute_name="geographic location (country and/or sea)" harmonized_name="geo_loc_name" display_name="geographic location">USA</Attribute>     <Attribute attribute_name="geographic location (latitude)">28.74</Attribute>     <Attribute attribute_name="geographic location (longitude)">-88.37</Attribute>     <Attribute attribute_name="investigation type" harmonized_name="investigation_type" display_name="investigation type">metagenome-assembled genome</Attribute>     <Attribute attribute_name="isolation source" harmonized_name="isolation_source" display_name="isolation source">marine metagenome</Attribute>     <Attribute attribute_name="metagenomic source">marine metagenome</Attribute>     <Attribute attribute_name="project name" harmonized_name="project_name" display_name="project name">In this study, we simulate the Deepwater Horizon oil spill in a laboratory system, with the indigenous seawater from the spill site and depth, Macondo oil, and dispersant (Corexit 9500) used during the spill. Successive samples were taken for chemical and metagenomic studies. From the assembled contigs, we isolated the dominant Oceanospirillaceae genome. This study showed that the succession of the microbial community coincide with the genomes’ use of hydrocarbon substrates. In general, n-alkane users will rise first, succeeded by mono-ring aromotics, then by multi-ring aromatics. The succession pattern was probably controlled by nutrient availability and the metabolic rates that the group of substrates can sustain. Multiple genomes of Colwellia, Cycloclasticus, members of Gamma-Proteobacteria, flavor bacteria and Rhodobacteria were also resolved, providing insights on potential cooperation or competition in the environment among closely related bacteria.</Attribute>     <Attribute attribute_name="sample derived from">SAMN04961124</Attribute>     <Attribute attribute_name="sample name" harmonized_name="sample_name" display_name="sample name">SRR3580187_bin.31_MetaBAT_v2.12.1_MAG</Attribute>     <Attribute attribute_name="sequencing method">Illumina HiSeq 2500</Attribute>     <Attribute attribute_name="taxonomic identity marker">multi-marker approach</Attribute>   </Attributes>   <Status status="live" when="2021-09-25T10:52:44.978"/> </BioSample> </BioSampleSet>
