SAMEA13511457 ERS11113462 uncultured Bacilli bacterium This sample represents an uncultured Bacilli bacterium MAG assembled and binned from ERR7737966. EBI Generic Generic.1.0 1 TRUE barrnap;0.9;--kingdom bac 1 1 0 2022-04-01 2022-04-01 ERC000011 SAMEA13511457 d__Bacteria;p__Firmicutes;c__Bacilli;o__RF39;f__UBA660;g__CAG-582;s__CAG-582 sp900760845 Stanford University School of Medicine stanford university school of medicine 2022-04-01T08:23:59Z 2022-04-01T08:23:59Z public 25.69 Reads (ERR7737966) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4559741) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15. REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-E-H_P_24_2355.113 Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs. metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz Reads from ERR7737966 were assembled into contigs (ERZ4559741). The following read sets from this study were the most similar to ERR7737966 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7737966,ERR7745970,ERR7738568,ERR7737971,ERR7738150,ERR7738197,ERR7738151,ERR7738552,ERR7746779,ERR7738179. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4559741. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin. MetaBAT2 v2.15; MAGpurify v2.1.0 2014-08-14 CheckM marker lineage: k__Bacteria (UID2329) 92.7 CheckM;1.1.2;lineage_wf 1.69 reads All reads in ERR7737966 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2). terrestrial biome human-associated habitat feces Tanzania 1287 -3.55 -34.58 ERR7737966_bin.113 ERR7737966_bin.113 metagenome-assembled genome human feces human gut metagenome 0.3868232224396608 0.3389538885065382 0.6386554621848739 https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 34 Metagenome sequencing of Hadza gut microbiome No https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 ERS2586222 REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-E-H_P_24_2355.113 Illumina NovaSeq 6000 tRNAscan-SE;2.0.9;-B GTDB-TK v1.7.0, r202 database, default parameters multi-marker approach 20