SAMEA13517129 ERS11119126 uncultured Bacilli bacterium This sample represents an uncultured Bacilli bacterium MAG assembled and binned from ERR7738560. EBI Generic Generic.1.0 1 TRUE barrnap;0.9;--kingdom bac 1 1 0 2022-04-01 2022-04-01 ERC000011 SAMEA13517129 d__Bacteria;p__Firmicutes;c__Bacilli;o__RF39;f__UBA660;g__CAG-710;s__CAG-710 sp000432595 Stanford University School of Medicine stanford university school of medicine 2022-04-01T08:24:39Z 2022-04-01T08:24:39Z public 128.89 Reads (ERR7738560) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567601) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15. REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Nepal_MoBio_Fiber-Hadza-Nepal_H_9_THA1076JZ.193 Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs. metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz Reads from ERR7738560 were assembled into contigs (ERZ4567601). The following read sets from this study were the most similar to ERR7738560 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738560,ERR7738572,ERR7745908,ERR7745755,ERR7738938,ERR7738451,ERR7738529,ERR7738621,ERR7745346,ERR7738558. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567601. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin. MetaBAT2 v2.15; MAGpurify v2.1.0 2016 CheckM marker lineage: k__Bacteria (UID2329) 94.94 CheckM;1.1.2;lineage_wf 0 reads All reads in ERR7738560 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2). terrestrial biome human-associated habitat feces Nepal 1400 28.17 84.25 ERR7738560_bin.193 ERR7738560_bin.193 metagenome-assembled genome human feces human gut metagenome 0.38095238095238093 1 0.6386554621848739 https://doi.org/10.1371/journal.pbio.2005396 33 Metagenome sequencing of Nepali gut microbiome No https://doi.org/10.1371/journal.pbio.2005396 https://doi.org/10.1371/journal.pbio.2005396 ERS2787413 REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Nepal_MoBio_Fiber-Hadza-Nepal_H_9_THA1076JZ.193 Illumina NovaSeq 6000 tRNAscan-SE;2.0.9;-B GTDB-TK v1.7.0, r202 database, default parameters multi-marker approach 20