SAMEA13470975 ERS11073034 uncultured Alphaproteobacteria bacterium This sample represents an uncultured Alphaproteobacteria bacterium MAG assembled and binned from ERR7746365. EBI Generic Generic.1.0 0 FALSE barrnap;0.9;--kingdom bac 0 0 0 2022-04-01 2022-04-01 ERC000011 SAMEA13470975 d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__RF32;f__CAG-239;g__RUG410;s__ Stanford University School of Medicine stanford university school of medicine 2022-04-01T08:25:53Z 2022-04-01T08:25:53Z public 24.21 Reads (ERR7746365) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4568022) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15. REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_E_16_1654.17 Many fragments with little to no review of assembly other than reporting of standard assembly statistics. metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz Reads from ERR7746365 were assembled into contigs (ERZ4568022). The following read sets from this study were the most similar to ERR7746365 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7746365,ERR7738605,ERR7738574,ERR7738461,ERR7738631,ERR7746321,ERR7745815,ERR7738569,ERR7738606,ERR7738241. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4568022. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin. MetaBAT2 v2.15; MAGpurify v2.1.0 2014-04-05 CheckM marker lineage: k__Bacteria (UID2565) 92.35 CheckM;1.1.2;lineage_wf 0 reads All reads in ERR7746365 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2). terrestrial biome human-associated habitat feces Tanzania 1287 -3.55 -34.58 ERR7745577_bin.128 ERR7746365_bin.17 metagenome-assembled genome human feces human gut metagenome https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 39 Metagenome sequencing of Hadza gut microbiome No https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 ERS9106184 REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_PheChl_Fiber-Hadza-Nepal_E_16_1654.17 Illumina NovaSeq 6000 tRNAscan-SE;2.0.9;-B GTDB-TK v1.7.0, r202 database, default parameters multi-marker approach 19