SAMEA13492891 ERS11094921 uncultured Sutterella sp. This sample represents an uncultured Sutterella sp. MAG assembled and binned from ERR7738583. EBI Generic Generic.1.0 0 FALSE barrnap;0.9;--kingdom bac 0 0 0 2022-04-01 2022-04-01 ERC000011 SAMEA13492891 d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Sutterella;s__Sutterella wadsworthensis Stanford University School of Medicine stanford university school of medicine 2022-04-01T08:25:12Z 2022-04-01T08:25:12Z public 46.38 Reads (ERR7738583) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567560) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15. REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Nepal_MoBio_Fiber-Hadza-Nepal_J_15_THA1075YZ.31 Many fragments with little to no review of assembly other than reporting of standard assembly statistics. metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz Reads from ERR7738583 were assembled into contigs (ERZ4567560). The following read sets from this study were the most similar to ERR7738583 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738583,ERR7745908,ERR7738662,ERR7738535,ERR7738513,ERR7745760,ERR7738645,ERR7738265,ERR7738451,ERR7738619. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567560. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin. MetaBAT2 v2.15; MAGpurify v2.1.0 2016 CheckM marker lineage: p__Proteobacteria (UID3887) 100 CheckM;1.1.2;lineage_wf 0 reads All reads in ERR7738583 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2). terrestrial biome human-associated habitat feces Nepal 1400 28.17 84.25 GUT_GENOME195227 ERR7738583_bin.31 metagenome-assembled genome human feces human gut metagenome https://doi.org/10.1371/journal.pbio.2005396 57 Metagenome sequencing of Nepali gut microbiome No https://doi.org/10.1371/journal.pbio.2005396 https://doi.org/10.1371/journal.pbio.2005396 ERS2787412 REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Nepal_MoBio_Fiber-Hadza-Nepal_J_15_THA1075YZ.31 Illumina NovaSeq 6000 tRNAscan-SE;2.0.9;-B GTDB-TK v1.7.0, r202 database, default parameters multi-marker approach 20