SAMEA13487752 ERS11089788 uncultured Lachnospiraceae bacterium This sample represents an uncultured Lachnospiraceae bacterium MAG assembled and binned from ERR7738562. EBI Generic Generic.1.0 0 FALSE barrnap;0.9;--kingdom bac 0 0 33.33 2022-04-01 2022-04-01 ERC000011 SAMEA13487752 d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__AM51-8;s__AM51-8 sp900761925 Stanford University School of Medicine stanford university school of medicine 2022-04-01T08:26:27Z 2022-04-01T08:26:27Z public 15.34 Reads (ERR7738562) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4558868) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15. REFINED_METABAT215_SUBJECT_CONTIGS_1500_ASSEMBLY_K77_MERGED__Pilot_MoBio_Fiber_G_23_1002.147 Many fragments with little to no review of assembly other than reporting of standard assembly statistics. metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz Reads from ERR7738562 were assembled into contigs (ERZ4558868). The following read sets from this study were the most similar to ERR7738562 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7738562,ERR7738597. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 2 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4558868. Depth information for all 2 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin. MetaBAT2 v2.15; MAGpurify v2.1.0 2017 CheckM marker lineage: f__Lachnospiraceae (UID1286) 90.26 CheckM;1.1.2;lineage_wf 0.97 reads All reads in ERR7738562 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2). terrestrial biome human-associated habitat feces USA 29 37.4275 -122.1697 GUT_GENOME255913 ERR7738562_bin.147 metagenome-assembled genome human feces human gut metagenome https://doi.org/10.1016/j.cell.2021.06.019 38 Metagenome sequencing of Californian gut microbiome No https://doi.org/10.1016/j.cell.2021.06.019 https://doi.org/10.1016/j.cell.2021.06.019 SRS9384124 REFINED_METABAT215_SUBJECT_CONTIGS_1500_ASSEMBLY_K77_MERGED__Pilot_MoBio_Fiber_G_23_1002.147 Illumina NovaSeq 6000 tRNAscan-SE;2.0.9;-B GTDB-TK v1.7.0, r202 database, default parameters multi-marker approach 17