SAMEA13471815 ERS11073873 uncultured Eubacteriales bacterium This sample represents an uncultured Eubacteriales bacterium MAG assembled and binned from ERR7745397. EBI Generic Generic.1.0 0 FALSE barrnap;0.9;--kingdom bac 0 2 0 2022-04-01 2022-04-01 ERC000011 SAMEA13471815 d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Oscillospirales;f__Acutalibacteraceae;g__UBA1227;s__UBA1227 sp900545655 Stanford University School of Medicine stanford university school of medicine 2022-04-01T08:26:25Z 2022-04-01T08:26:25Z public 21.23 Reads (ERR7745397) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4561073) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15. REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_D_23_1576.107 Many fragments with little to no review of assembly other than reporting of standard assembly statistics. metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz Reads from ERR7745397 were assembled into contigs (ERZ4561073). The following read sets from this study were the most similar to ERR7745397 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7745397,ERR7738213,ERR7737953,ERR7738176,ERR7738531,ERR7745937,ERR7745648,ERR7738561,ERR7746785,ERR7738606. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4561073. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin. MetaBAT2 v2.15; MAGpurify v2.1.0 2014-01-30 CheckM marker lineage: o__Clostridiales (UID1212) 96.64 CheckM;1.1.2;lineage_wf 0.67 reads All reads in ERR7745397 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2). terrestrial biome human-associated habitat feces Tanzania 1287 -3.55 -34.58 GUT_GENOME222400 ERR7745397_bin.107 metagenome-assembled genome human feces human gut metagenome 0.9411764705882353 https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 42 Metagenome sequencing of Hadza gut microbiome No https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 ERS2585825 REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-A-D_D_23_1576.107 Illumina NovaSeq 6000 tRNAscan-SE;2.0.9;-B GTDB-TK v1.7.0, r202 database, default parameters multi-marker approach 19