SAMEA13486162 ERS11088201 uncultured Clostridia bacterium This sample represents an uncultured Clostridia bacterium MAG assembled and binned from ERR7739005. EBI Generic Generic.1.0 0 FALSE barrnap;0.9;--kingdom bac 0 0 60 2022-04-01 2022-04-01 ERC000011 SAMEA13486162 d__Bacteria;p__Firmicutes_A;c__Clostridia_A;o__Christensenellales;f__CAG-74;g__UMGS1633;s__UMGS1633 sp900553645 Stanford University School of Medicine stanford university school of medicine 2022-04-01T08:26:20Z 2022-04-01T08:26:20Z public 14.06 Reads (ERR7739005) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4567239) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15. REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Nepal_MoBio_Fiber-Hadza-Nepal_F_1_RAJ0018YZ.133 Many fragments with little to no review of assembly other than reporting of standard assembly statistics. metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz Reads from ERR7739005 were assembled into contigs (ERZ4567239). The following read sets from this study were the most similar to ERR7739005 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7739005,ERR7745858,ERR7745346,ERR7745755,ERR7738572,ERR7738575,ERR7738662,ERR7745760,ERR7738535,ERR7738529. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4567239. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin. MetaBAT2 v2.15; MAGpurify v2.1.0 2016 CheckM marker lineage: c__Clostridia (UID1118) 85.28 CheckM;1.1.2;lineage_wf 4.03 reads All reads in ERR7739005 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2). terrestrial biome human-associated habitat feces Nepal 1400 28.17 84.25 ERR7738587_bin.165 ERR7738587_bin.165 metagenome-assembled genome human feces human gut metagenome https://doi.org/10.1371/journal.pbio.2005396 41 Metagenome sequencing of Nepali gut microbiome No https://doi.org/10.1371/journal.pbio.2005396 https://doi.org/10.1371/journal.pbio.2005396 ERS2787375 REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Nepal_MoBio_Fiber-Hadza-Nepal_F_1_RAJ0018YZ.133 Illumina NovaSeq 6000 tRNAscan-SE;2.0.9;-B GTDB-TK v1.7.0, r202 database, default parameters multi-marker approach 17