SAMEA13483969 ERS11086010 uncultured Clostridium sp. This sample represents an uncultured Clostridium sp. MAG assembled and binned from ERR7746768. EBI Generic Generic.1.0 0 FALSE barrnap;0.9;--kingdom bac 0 3 0 2022-04-01 2022-04-01 ERC000011 SAMEA13483969 d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_P;s__Clostridium_P perfringens Stanford University School of Medicine stanford university school of medicine 2022-04-01T08:24:03Z 2022-04-01T08:24:03Z public 19 Reads (ERR7746768) were merged using BBMerge (rem k=62 extend2=50 ecct vstrict) and then mapped to assembled contigs >=1500bp (ERZ4561218) using Bowtie2 v2.3.4 (--very-sensitive -X 1000). Coverage was calculated using jgi_summarize_bam_contig_depths (default parameters) from MetaBAT2 v2.15. REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-I-L_L_5_1268.38 Many fragments with little to no review of assembly other than reporting of standard assembly statistics. metaSPAdes;3.13.1;-k 21,33,55,77 --merged BBMerge.fq.gz -1 R1.fq.gz -2 R2.fq.gz Reads from ERR7746768 were assembled into contigs (ERZ4561218). The following read sets from this study were the most similar to ERR7746768 as determined by MASH (-r -m 2 -s 100000 -k 32): ERR7746768,ERR7745621,ERR7738153,ERR7745876,ERR7745561,ERR7746794,ERR7738155,ERR7745539,ERR7737955,ERR7738236. Following processing using BBMerge (rem k=62 extend2=50 ecct vstrict), reads from each of these 10 samples were mapped using bowtie2 v2.3.4 (--very-sensitive -X 1000) onto contigs >=1500bp from ERZ4561218. Depth information for all 10 mapped samples was utilized by MetaBAT2 2.15 for genome binning. Contigs flagged by any module in MAGpurify 2.1.0 (--weighted mode for gc_content, tetra_freq, and coverage modules; conspecific database augmented by high-quality MAGs from this study) were removed from the bin. MetaBAT2 v2.15; MAGpurify v2.1.0 2013-10-25 CheckM marker lineage: o__Clostridiales (UID1375) 100 CheckM;1.1.2;lineage_wf 0 reads All reads in ERR7746768 failed to map to hg19 (with custom masking) using BBMap v38.86 (minid=0.95 maxindel=3 bwr=0.16 bw=12 minhits=2). terrestrial biome human-associated habitat feces Tanzania 1287 -3.55 -34.58 GUT_GENOME142391 ERR7738532_bin.124 metagenome-assembled genome human feces human gut metagenome 0.8151260504201681 https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 57 Metagenome sequencing of Hadza gut microbiome No https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 https://doi.org/10.1126/science.aan4834; https://doi.org/10.1080/19490976.2018.1494103 ERS2585686 REFINED_METABAT215_TOP10_CONTIGS_1500_ASSEMBLY_K77_MERGED__Hadza_MoBio_hadza-I-L_L_5_1268.38 Illumina NovaSeq 6000 tRNAscan-SE;2.0.9;-B GTDB-TK v1.7.0, r202 database, default parameters multi-marker approach 18