[2023-06-05 13:29:57,691] [INFO] DFAST_QC pipeline started.
[2023-06-05 13:29:57,695] [INFO] DFAST_QC version: 0.5.7
[2023-06-05 13:29:57,695] [INFO] DQC Reference Directory: /var/lib/cwl/stgd80b40bb-1ce6-45c3-80a8-0527652150a0/dqc_reference
[2023-06-05 13:29:59,160] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-05 13:29:59,161] [INFO] Task started: Prodigal
[2023-06-05 13:29:59,161] [INFO] Running command: gunzip -c /var/lib/cwl/stg472b71a5-5269-485c-b525-b83aafa669e9/GCA_934868665.1_MTG244_bin.47.fa_genomic.fna.gz | prodigal -d GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/cds.fna -a GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-05 13:30:21,058] [INFO] Task succeeded: Prodigal
[2023-06-05 13:30:21,059] [INFO] Task started: HMMsearch
[2023-06-05 13:30:21,059] [INFO] Running command: hmmsearch --tblout GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd80b40bb-1ce6-45c3-80a8-0527652150a0/dqc_reference/reference_markers.hmm GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/protein.faa > /dev/null
[2023-06-05 13:30:21,307] [INFO] Task succeeded: HMMsearch
[2023-06-05 13:30:21,308] [INFO] Found 6/6 markers.
[2023-06-05 13:30:21,343] [INFO] Query marker FASTA was written to GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/markers.fasta
[2023-06-05 13:30:21,344] [INFO] Task started: Blastn
[2023-06-05 13:30:21,344] [INFO] Running command: blastn -query GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/markers.fasta -db /var/lib/cwl/stgd80b40bb-1ce6-45c3-80a8-0527652150a0/dqc_reference/reference_markers.fasta -out GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 13:30:21,917] [INFO] Task succeeded: Blastn
[2023-06-05 13:30:21,921] [INFO] Selected 11 target genomes.
[2023-06-05 13:30:21,922] [INFO] Target genome list was writen to GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/target_genomes.txt
[2023-06-05 13:30:21,923] [INFO] Task started: fastANI
[2023-06-05 13:30:21,923] [INFO] Running command: fastANI --query /var/lib/cwl/stg472b71a5-5269-485c-b525-b83aafa669e9/GCA_934868665.1_MTG244_bin.47.fa_genomic.fna.gz --refList GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/target_genomes.txt --output GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/fastani_result.tsv --threads 1
[2023-06-05 13:30:31,695] [INFO] Task succeeded: fastANI
[2023-06-05 13:30:31,695] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd80b40bb-1ce6-45c3-80a8-0527652150a0/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-05 13:30:31,695] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd80b40bb-1ce6-45c3-80a8-0527652150a0/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-05 13:30:31,705] [INFO] Found 10 fastANI hits (5 hits with ANI > threshold)
[2023-06-05 13:30:31,705] [INFO] The taxonomy check result is classified as 'inconclusive'.
[2023-06-05 13:30:31,706] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Bacteroides uniformis	strain=ATCC 8492	GCA_000154205.1	820	820	type	True	98.3123	1046	1223	95	inconclusive
Bacteroides uniformis	strain=ATCC 8492	GCA_025147485.1	820	820	type	True	98.3094	1054	1223	95	inconclusive
Bacteroides uniformis	strain=DSM 6597	GCA_900107315.1	820	820	type	True	98.248	1055	1223	95	inconclusive
Bacteroides uniformis	strain=FDAARGOS_901	GCA_016117815.1	820	820	type	True	98.2464	1067	1223	95	inconclusive
Bacteroides humanifaecis	strain=KGMB07931	GCA_017309675.2	2792859	2792859	type	True	95.9015	934	1223	95	inconclusive
Bacteroides rodentium	strain=JCM 16496	GCA_000614125.1	691816	691816	type	True	94.6707	881	1223	95	below_threshold
Bacteroides muris	strain=KH365_2	GCA_024704915.1	2937417	2937417	type	True	92.3234	811	1223	95	below_threshold
Bacteroides stercoris	strain=ATCC 43183	GCA_025147325.1	46506	46506	suspected-type	True	80.2402	480	1223	95	below_threshold
Bacteroides propionicigenes	strain=NSJ-90	GCA_018390535.1	2834112	2834112	type	True	79.9496	465	1223	95	below_threshold
Bacteroides stercorirosoris	strain=JCM 17103	GCA_000614165.1	871324	871324	type	True	79.2283	480	1223	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-05 13:30:31,708] [INFO] DFAST Taxonomy check result was written to GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/tc_result.tsv
[2023-06-05 13:30:31,708] [INFO] ===== Taxonomy check completed =====
[2023-06-05 13:30:31,709] [INFO] ===== Start completeness check using CheckM =====
[2023-06-05 13:30:31,709] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd80b40bb-1ce6-45c3-80a8-0527652150a0/dqc_reference/checkm_data
[2023-06-05 13:30:31,710] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-05 13:30:31,747] [INFO] Task started: CheckM
[2023-06-05 13:30:31,748] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/checkm_input GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/checkm_result
[2023-06-05 13:31:29,358] [INFO] Task succeeded: CheckM
[2023-06-05 13:31:29,360] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 79.17%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-05 13:31:29,381] [INFO] ===== Completeness check finished =====
[2023-06-05 13:31:29,382] [INFO] ===== Start GTDB Search =====
[2023-06-05 13:31:29,383] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/markers.fasta)
[2023-06-05 13:31:29,383] [INFO] Task started: Blastn
[2023-06-05 13:31:29,383] [INFO] Running command: blastn -query GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/markers.fasta -db /var/lib/cwl/stgd80b40bb-1ce6-45c3-80a8-0527652150a0/dqc_reference/reference_markers_gtdb.fasta -out GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-05 13:31:30,182] [INFO] Task succeeded: Blastn
[2023-06-05 13:31:30,186] [INFO] Selected 13 target genomes.
[2023-06-05 13:31:30,186] [INFO] Target genome list was writen to GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/target_genomes_gtdb.txt
[2023-06-05 13:31:30,191] [INFO] Task started: fastANI
[2023-06-05 13:31:30,191] [INFO] Running command: fastANI --query /var/lib/cwl/stg472b71a5-5269-485c-b525-b83aafa669e9/GCA_934868665.1_MTG244_bin.47.fa_genomic.fna.gz --refList GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/target_genomes_gtdb.txt --output GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-05 13:31:40,814] [INFO] Task succeeded: fastANI
[2023-06-05 13:31:40,825] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-05 13:31:40,825] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000154205.1	s__Bacteroides uniformis	98.3123	1046	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	98.42	95.87	0.83	0.69	301	conclusive
GCF_000614125.1	s__Bacteroides rodentium	94.6707	881	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004793475.1	s__Bacteroides sp002491635	92.2614	819	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	99.51	98.46	0.94	0.83	13	-
GCA_905197435.1	s__Bacteroides sp905197435	87.8503	583	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902388495.1	s__Bacteroides sp902388495	81.6421	608	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	99.99	99.99	0.98	0.98	2	-
GCF_000195635.1	s__Bacteroides fluxus	81.6016	584	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	99.66	99.32	0.94	0.88	3	-
GCA_905203765.1	s__Bacteroides sp905203765	81.0271	471	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900129655.1	s__Bacteroides clarus	80.1214	474	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	98.75	98.47	0.87	0.82	18	-
GCF_003438615.1	s__Bacteroides sp003545565	79.9045	457	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	99.56	99.02	0.91	0.88	7	-
GCF_000155815.1	s__Bacteroides eggerthii	79.777	457	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	98.86	98.28	0.85	0.74	51	-
GCF_000186225.1	s__Bacteroides helcogenes	79.6946	490	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905198575.1	s__Phocaeicola sp905198575	77.5982	61	1223	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-05 13:31:40,827] [INFO] GTDB search result was written to GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/result_gtdb.tsv
[2023-06-05 13:31:40,828] [INFO] ===== GTDB Search completed =====
[2023-06-05 13:31:40,832] [INFO] DFAST_QC result json was written to GCA_934868665.1_MTG244_bin.47.fa_genomic.fna/dqc_result.json
[2023-06-05 13:31:40,832] [INFO] DFAST_QC completed!
[2023-06-05 13:31:40,832] [INFO] Total running time: 0h1m43s