SAMEA14036485 ERS11639767 uncultured Microbacterium sp. This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR3519527 of study ERP117308. EBI Generic Generic.1.0 ERC000047 2023-01-03 2023-01-03 SAMEA14036485 EBI European Bioinformatics Institute 2023-01-03T00:32:01Z 2023-01-03T00:32:01Z public ERR3519527_bin.43_CONCOCT_v1.1_MAG Many fragments with little to no review of assembly other than reporting of standard assembly statistics metaSPAdes v3.13.1 Default CONCOCT v1.1 EMG broker account, EMBL-EBI 2017-01-01 94.76 CheckM 1.43 Engineered Wastewater Wastewater United Kingdom not provided not provided metagenome-assembled genome wastewater metagenome wastewater metagenome Hospitals are nodal points for antimicrobial consumption and identification of resistant pathogens and therefore hospital wastewater is a potential major source of AMR. This study uses metagenomics to study how the abundances of resistance genes in hospital wastewater are related to clinical activity within different buildings of the hospital. Sewage was collected over a 24-hour period from multiple wastewater collection points representing different specialties within a tertiary hospital site and simultaneously from community sewage works. High throughput shotgun sequencing was performed using Illumina HiSeq4000. Sequence reads were assigned taxonomically and to the AMR genes in the ResFinder database. The measured AMR gene abundances were then correlated to hospital antimicrobial usage (AMU), other data on clinical activity such as patient/ward characteristics and resistance prevalence in clinical isolates. SAMEA5954000 ERR3519527_bin.43_CONCOCT_v1.1_MAG uncultured Microbacterium sp. Illumina HiSeq 4000 multi-marker approach