<?xml version="1.0" ?>
<BioSampleSet><BioSample access="public" publication_date="2023-01-03T00:00:00.000" last_update="2023-04-12T11:38:35.000" submission_date="2023-01-04T08:29:41.516" id="32557727" accession="SAMEA14075813">   <Ids>     <Id db="BioSample" is_primary="1">SAMEA14075813</Id>     <Id db="SRA">ERS11679041</Id>   </Ids>   <Description>     <Title>Metagenome-assembled genome: SRR2240840_bin.53_CONCOCT_v1.1_MAG</Title>     <Organism taxonomy_id="851" taxonomy_name="Fusobacterium nucleatum">       <OrganismName>Fusobacterium nucleatum</OrganismName>     </Organism>     <Comment>       <Paragraph>This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR2240840 of study SRP056641.</Paragraph>     </Comment>   </Description>   <Owner>     <Name>EBI</Name>   </Owner>   <Models>     <Model>Generic</Model>   </Models>   <Package display_name="Generic">Generic.1.0</Package>   <Attributes>     <Attribute attribute_name="ENA-CHECKLIST">ERC000047</Attribute>     <Attribute attribute_name="ENA-FIRST-PUBLIC">2023-01-03</Attribute>     <Attribute attribute_name="ENA-LAST-UPDATE">2023-01-03</Attribute>     <Attribute attribute_name="External Id">SAMEA14075813</Attribute>     <Attribute attribute_name="INSDC center alias">EBI</Attribute>     <Attribute attribute_name="INSDC center name">European Bioinformatics Institute</Attribute>     <Attribute attribute_name="INSDC first public">2023-01-03T00:32:50Z</Attribute>     <Attribute attribute_name="INSDC last update">2023-01-03T00:32:50Z</Attribute>     <Attribute attribute_name="INSDC status">public</Attribute>     <Attribute attribute_name="Submitter Id">SRR2240840_bin.53_CONCOCT_v1.1_MAG</Attribute>     <Attribute attribute_name="assembly quality">Many fragments with little to no review of assembly other than reporting of standard assembly statistics</Attribute>     <Attribute attribute_name="assembly software">spadesv3.11.1</Attribute>     <Attribute attribute_name="binning parameters">Default</Attribute>     <Attribute attribute_name="binning software">CONCOCT v1.1</Attribute>     <Attribute attribute_name="broker name">EMG broker account, EMBL-EBI</Attribute>     <Attribute attribute_name="collection date" harmonized_name="collection_date" display_name="collection date">not provided</Attribute>     <Attribute attribute_name="completeness score">94.78</Attribute>     <Attribute attribute_name="completeness software">CheckM</Attribute>     <Attribute attribute_name="contamination score">0.0</Attribute>     <Attribute attribute_name="environment (biome)" harmonized_name="env_broad_scale" display_name="broad-scale environmental context">Mixed</Attribute>     <Attribute attribute_name="environment (feature)" harmonized_name="env_local_scale" display_name="local-scale environmental context">Mixed</Attribute>     <Attribute attribute_name="environment (material)" harmonized_name="env_medium" display_name="environmental medium">Mixed</Attribute>     <Attribute attribute_name="geographic location (country and/or sea)" harmonized_name="geo_loc_name" display_name="geographic location">not provided</Attribute>     <Attribute attribute_name="geographic location (latitude)">not provided</Attribute>     <Attribute attribute_name="geographic location (longitude)">not provided</Attribute>     <Attribute attribute_name="investigation type" harmonized_name="investigation_type" display_name="investigation type">metagenome-assembled genome</Attribute>     <Attribute attribute_name="isolation_source" harmonized_name="isolation_source" display_name="isolation source">metagenome</Attribute>     <Attribute attribute_name="metagenomic source">metagenome</Attribute>     <Attribute attribute_name="project name" harmonized_name="project_name" display_name="project name">The samples within this Bioproject represent the third phase (Phase III) of metagenomic sequencing (whole genome shotgun (WGS) sequencing) of microbial communities from specimens collected as a part of the NIH-supported Human Microbiome Project (HMP Consortium A framework for human microbiome research. Nature. 2012 Jun 13;486(7402):215-21. doi: 10.1038/nature11209). The sample collection represents a unique reference resource of microbiome samples from healthy adults largely residing in the USA at the time of sample collection. In the HMP Phase III component the goal has been to supplement previous WGS sequencing by generating additional sequence from as many of the specimens in the HMP collection as possible that had not been sequenced in the previous HMP Phase I and Phase II sequencing. In Phase III, a focus was placed on six of the 18 body sites: Stool (gut), Buccal mucosa (oral), Supragingival plaque (oral), Tongue dorsum (oral), Anterior nares (nasal), Posterior fornix (vaginal) and the goal was to further capture as many of the longitudinal samples (second and third visits by the same individual) as possible. From genomic DNA extracted from the collected specimens, WGS sequence was produced from libraries constructed from using a NexteraXT library construction protocol. All libraries were sequenced using a 100bp paired end approach using the Illumina HiSeq 2000 platform.</Attribute>     <Attribute attribute_name="sample derived from">SAMN03351558</Attribute>     <Attribute attribute_name="sample name" harmonized_name="sample_name" display_name="sample name">SRR2240840_bin.53_CONCOCT_v1.1_MAG</Attribute>     <Attribute attribute_name="scientific_name">Fusobacterium nucleatum</Attribute>     <Attribute attribute_name="sequencing method">Illumina HiSeq 2000</Attribute>     <Attribute attribute_name="taxonomic identity marker">multi-marker approach</Attribute>   </Attributes>   <Status status="live" when="2023-01-05T08:27:51.847"/> </BioSample> </BioSampleSet>
