[2023-06-19 00:41:04,150] [INFO] DFAST_QC pipeline started.
[2023-06-19 00:41:04,154] [INFO] DFAST_QC version: 0.5.7
[2023-06-19 00:41:04,154] [INFO] DQC Reference Directory: /var/lib/cwl/stg3f2200b2-21de-4d9e-8621-73950730f2f3/dqc_reference
[2023-06-19 00:41:06,273] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-19 00:41:06,274] [INFO] Task started: Prodigal
[2023-06-19 00:41:06,274] [INFO] Running command: gunzip -c /var/lib/cwl/stgc4e6009d-8920-4a83-ae70-b7ebcfbfb9c8/GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna.gz | prodigal -d GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/cds.fna -a GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-19 00:41:12,256] [INFO] Task succeeded: Prodigal
[2023-06-19 00:41:12,257] [INFO] Task started: HMMsearch
[2023-06-19 00:41:12,257] [INFO] Running command: hmmsearch --tblout GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3f2200b2-21de-4d9e-8621-73950730f2f3/dqc_reference/reference_markers.hmm GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/protein.faa > /dev/null
[2023-06-19 00:41:12,503] [INFO] Task succeeded: HMMsearch
[2023-06-19 00:41:12,504] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgc4e6009d-8920-4a83-ae70-b7ebcfbfb9c8/GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna.gz]
[2023-06-19 00:41:12,535] [INFO] Query marker FASTA was written to GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/markers.fasta
[2023-06-19 00:41:12,536] [INFO] Task started: Blastn
[2023-06-19 00:41:12,536] [INFO] Running command: blastn -query GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/markers.fasta -db /var/lib/cwl/stg3f2200b2-21de-4d9e-8621-73950730f2f3/dqc_reference/reference_markers.fasta -out GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 00:41:13,107] [INFO] Task succeeded: Blastn
[2023-06-19 00:41:13,114] [INFO] Selected 13 target genomes.
[2023-06-19 00:41:13,114] [INFO] Target genome list was writen to GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/target_genomes.txt
[2023-06-19 00:41:13,136] [INFO] Task started: fastANI
[2023-06-19 00:41:13,136] [INFO] Running command: fastANI --query /var/lib/cwl/stgc4e6009d-8920-4a83-ae70-b7ebcfbfb9c8/GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna.gz --refList GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/target_genomes.txt --output GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/fastani_result.tsv --threads 1
[2023-06-19 00:41:18,701] [INFO] Task succeeded: fastANI
[2023-06-19 00:41:18,702] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3f2200b2-21de-4d9e-8621-73950730f2f3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-19 00:41:18,702] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3f2200b2-21de-4d9e-8621-73950730f2f3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-19 00:41:18,704] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-19 00:41:18,704] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-19 00:41:18,705] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-19 00:41:18,706] [INFO] DFAST Taxonomy check result was written to GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/tc_result.tsv
[2023-06-19 00:41:18,707] [INFO] ===== Taxonomy check completed =====
[2023-06-19 00:41:18,707] [INFO] ===== Start completeness check using CheckM =====
[2023-06-19 00:41:18,707] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3f2200b2-21de-4d9e-8621-73950730f2f3/dqc_reference/checkm_data
[2023-06-19 00:41:18,710] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-19 00:41:18,739] [INFO] Task started: CheckM
[2023-06-19 00:41:18,739] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/checkm_input GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/checkm_result
[2023-06-19 00:41:44,360] [INFO] Task succeeded: CheckM
[2023-06-19 00:41:44,362] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 89.90%
Contamintation: 7.36%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-19 00:41:44,414] [INFO] ===== Completeness check finished =====
[2023-06-19 00:41:44,414] [INFO] ===== Start GTDB Search =====
[2023-06-19 00:41:44,415] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/markers.fasta)
[2023-06-19 00:41:44,415] [INFO] Task started: Blastn
[2023-06-19 00:41:44,415] [INFO] Running command: blastn -query GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/markers.fasta -db /var/lib/cwl/stg3f2200b2-21de-4d9e-8621-73950730f2f3/dqc_reference/reference_markers_gtdb.fasta -out GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-19 00:41:45,197] [INFO] Task succeeded: Blastn
[2023-06-19 00:41:45,204] [INFO] Selected 21 target genomes.
[2023-06-19 00:41:45,204] [INFO] Target genome list was writen to GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/target_genomes_gtdb.txt
[2023-06-19 00:41:45,213] [INFO] Task started: fastANI
[2023-06-19 00:41:45,213] [INFO] Running command: fastANI --query /var/lib/cwl/stgc4e6009d-8920-4a83-ae70-b7ebcfbfb9c8/GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna.gz --refList GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/target_genomes_gtdb.txt --output GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-19 00:41:50,001] [INFO] Task succeeded: fastANI
[2023-06-19 00:41:50,005] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-19 00:41:50,006] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_001786995.1	s__2-01-FULL-33-17 sp001786995	78.7314	67	493	d__Bacteria;p__Patescibacteria;c__Paceibacteria;o__UBA9983_A;f__UBA9973;g__2-01-FULL-33-17	95.0	99.73	99.73	0.84	0.84	2	-
--------------------------------------------------------------------------------
[2023-06-19 00:41:50,011] [INFO] GTDB search result was written to GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/result_gtdb.tsv
[2023-06-19 00:41:50,011] [INFO] ===== GTDB Search completed =====
[2023-06-19 00:41:50,015] [INFO] DFAST_QC result json was written to GCA_937895515.1_SRR6231146_bin.88_CONCOCT_v1.1_MAG_genomic.fna/dqc_result.json
[2023-06-19 00:41:50,015] [INFO] DFAST_QC completed!
[2023-06-19 00:41:50,015] [INFO] Total running time: 0h0m46s