SAMEA14080624 ERS11683880 uncultured Sutterella sp. This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR318692 of study ERP003671. EBI Generic Generic.1.0 ERC000047 2023-01-03 2023-01-03 SAMEA14080624 EBI European Bioinformatics Institute 2023-01-03T00:33:00Z 2023-01-03T00:33:00Z public ERR318692_bin.131_CONCOCT_v1.1_MAG Many fragments with little to no review of assembly other than reporting of standard assembly statistics metaSPAdes v3.12.0 Default CONCOCT v1.1 EMG broker account, EMBL-EBI 2006-05-01 96.25 CheckM 1.25 Host-associated Mammals Digestive system USA 40.103878 -88.2252 metagenome-assembled genome gut metagenome gut metagenome Despite being obligate carnivores, cats are fed a wide range of dietary macronutrients within commercial diets. Little is known regarding the feline gastrointestinal microbiome and how it is impacted by diet. Using 16S RNA analysis, changes in diet (protein:carbohydrate ratio) do affect fecal microbiota of growing kittens. However, to investigate potential functional differences deep Illumina shotgun sequencing is required and a study of microbial structure and function in these animals is reported here. This is the largest and most comprehensive analysis of the feline gastrointestinal microbiome to date. It provides an overview of the taxa and gene functions present and how they are impacted by the dietary protein: carbohydrate ratio. The more diverse structure of the high-protein diet might reflect a more specialized microbiome. As anticipated, our permutation approach reduced the number of pathways to a more manageable set. The reduced data set identified six pathways readily interpretable to the study design. SAMEA2154425 ERR318692_bin.131_CONCOCT_v1.1_MAG uncultured Sutterella sp. Illumina HiSeq 2000 multi-marker approach